Lantibiotic Lacticin 3147 A1 (Ltna1) (ORF00035) Lactococcus Lactis Subsp. Lactis Recombinant Protein

Function: Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores. When present individually lacticin 3147 A1 exhibits strong activity towards L.lactis strain AM2, weak activity towards L.lactis strain HP and no activity towards L.lactis strain IFPL359, but when combined with lacticin 3147 A2 it displays strong activity towards all three strains. Ref.2 Ref.4

Subcellular location: Secreted Ref.2 Ref.4.

Post-translational modification: Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor. Ref.3It is not established whether the 2,3-didehydrobutyrines are the E- or Z-isomers (Ref.3 and Ref.4). In the NMR model they were assumed to be the Z-isomer.

Mass spectrometry: Molecular mass is 3322 Da from positions 30 - 59. Determined by PD. Ref.2Molecular mass is 3306.69 Da from positions 30 - 59. Determined by MALDI. Ref.3Molecular mass is 3322.34 Da from positions 30 - 59. Determined by ESI. Ref.4