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UDP-N-Acetylmuramate:l-Alanyl-Gamma-D-Glutamyl-Meso-Diaminopimelate Ligase (mpl) Escherichia Coli Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1155330

MBS1155330 is a recombinant protein targeting mpl. Also known as Recombinant Escherichia coli UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (mpl). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$1230 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (mpl), Recombinant Protein
Full Product Name
Recombinant Escherichia coli UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (mpl)
Product Gene Name
Sequence
MRIHILGICG TFMGGLAMLA RQLGHEVTGS DANVYPPMST LLEKQGIELI QGYDASQLEP QPDLVIIGNA MTRGNPCVEA VLEKNIPYMS GPQWLHDFVL RDRWVLAVAG THGKTTTAGM ATWILEQCGY KPGFVIGGVP GNFEVSAHLG ESDFFVIEAD EYDCAFFDKR SKFVHYCPRT LILNNLEFDH ADIFDDLKAI QKQFHHLVRI VPGQGRIIWP ENDINLKQTM AMGCWSEQEL VGEQGHWQAK KLTTDASEWE VLLDGEKVGE VKWSLVGEHN MHNGLMAIAA ARHVGVAPAD AANALGSFIN ARRRLELRGE ANGVTVYDDF AHHPTAILAT LAALRGKVGG TARIIAVLEP RSNTMKMGIC KDDLAPSLGR ADEVFLLQPA HIPWQVAEVA EACVQPAHWS GDVDTLADMV VKTAQPGDHI LVMSNGGFGG IHQKLLDGLA KKAEAAQ
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
49,874 Da
Sequence Positions
1-457, full length protein
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for P37773
Species
Escherichia coli (strain K12)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of mpl recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for mpl. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase
NCBI Official Symbol
mpl
NCBI Official Synonym Symbols
ECK4228; JW4192; tpl; yjfG
UniProt Protein Name
UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptandioate ligase
UniProt Synonym Protein Names
Murein peptide ligase
UniProt Gene Name
mpl
NCBI Protein Information
UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase

NCBI Summary for mpl

RpoS regulon. [More information is available at EcoGene: EG12440]. The mpl gene encodes UDP-N-acetylmuramate:L-alanyl--D-glutamyl-meso-diaminopimelate ligase, which acts in murein recycling by allowing ligation of the tripeptide L-Ala-D-Glu-mesoA2pm with UDP-MurNAc and reincorporation into peptidoglycan. [More information is available at EcoCyc: EG12440].
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UniProt Comments for mpl

Reutilizes the intact tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramate. The enzyme can also use the tetrapeptide L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioyl-D-alanine or the pentapeptide L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptandioyl-D-alanyl-D-alanine in vivo and in vitro.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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