Oxygen-Insensitive NADPH Nitroreductase (nfsA) Escherichia Coli (Strain K12) Recombinant Protein

Reduction of nitroaromatic compounds using NADH. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major component of the oxygen-insensitive nitroreductase activity in E Coli.

A general genetic approach in Escherichia coli for determining the mechanism(s) of action of tumoricidal agents application to DMP 840, a tumoricidal agent.Chatterjee P.K., Sternberg N.L.Proc. Natl. Acad. Sci. U.S.A. 92:8950-8954(1995) Biochemical characterization of NfsA, the Escherichia coli major nitroreductase exhibiting a high amino acid sequence homology to Frp, a Vibrio harveyi flavin oxidoreductase.Zenno S., Koike H., Kumar A.N., Jayaraman R., Tanokura M., Saigo K.J. Bacteriol. 178:4508-4514(1996) A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.DNA Res. 3:137-155(1996) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Characterization of the gene rimK responsible for the addition of glutamic acid residues to the C-terminus of ribosomal protein S6 in Escherichia coli K12.Kang W.-K., Icho T., Isono S., Kitakawa M., Isono K.Mol. Gen. Genet. 217:281-288(1989) Conversion of NfsA, the major Escherichia coli nitroreductase, to a flavin reductase with an activity similar to that of Frp, a flavin reductase in Vibrio harveyi, by a single amino acid substitution.Zenno S., Kobori T., Tanokura M., Saigo K.J. Bacteriol. 180:422-425(1998) Structure and site-directed mutagenesis of a flavoprotein from Escherichia coli that reduces nitrocompounds alteration of pyridine nucleotide binding by a single amino acid substitution.Kobori T., Sasaki H., Lee W.C., Zenno S., Saigo K., Murphy M.E., Tanokura M.J. Biol. Chem. 276:2816-2823(2001)

Small volumes of nfsA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1159801 is a recombinant protein targeting nfsA. Also known as Recombinant Escherichia coli (strain K12) Oxygen-insensitive NADPH nitroreductase. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.