Genome Polyprotein (NS1) Dengue Virus Type 2 Recombinant Protein

Function: Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes

By similarity.Non-structural protein 1 is involved in virus replication and regulation of the innate immune response. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome

By similarity.Non-structural protein 2A may be involved viral RNA replication and capsid assembly

Potential.Non-structural protein 2B is a required cofactor for the serine protease function of NS3

By similarity.Serine protease NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, performs its autocleavage and cleaves the polyprotein at dibasic sites in the cytoplasm: C-prM, NS2A-NS2B, NS2B-NS3, NS3-NS4A, NS4A-2K and NS4B-NS5. NS3 RNA helicase binds RNA and unwinds dsRNA in the 3' to 5' direction

By similarity.

Catalytic activity: Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.NTP + H2O = NDP + phosphate.ATP + H2O = ADP + phosphate.

Subunit structure: In immature particles, there are 60 icosaedrally organized trimeric spikes on the surface. Each spike consists of three heterodimers of envelope protein M precursor (prM) and envelope protein E. NS1 forms homodimers as well as homohexamers when secreted. NS1 may interact with NS4A. NS3 and NS2B form a heterodimer. NS3 is the catalytic subunit, whereas NS2B strongly stimulates the latter, acting as a cofactor. In the absence of the NS2B, NS3 protease is unfolded and inactive

By similarity.

Subcellular location: Envelope protein E: Virion membrane; Multi-pass membrane protein

By similarity. Host endoplasmic reticulum membrane; Multi-pass membrane protein

By similarity. Non-structural protein 1: Secreted. Host endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side

By similarity. Non-structural protein 2A-alpha: Host endoplasmic reticulum membrane; Multi-pass membrane protein

Potential. Non-structural protein 2A: Host endoplasmic reticulum membrane; Multi-pass membrane protein

Potential. Serine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side

By similarity. Note: Remains non-covalently associated to NS3 protease

By similarity.

Post-translational modification: Specific enzymatic cleavages in vivo yield mature proteins

By similarity.

Sequence similarities: Contains 1 helicase ATP-binding domain.Contains 1 helicase C-terminal domain.Contains 1 peptidase S7 domain.