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Putative Type II Secretion System Protein F (gspF) Escherichia Coli Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1164348

MBS1164348 is a recombinant protein targeting gspF. Also known as Recombinant Escherichia coli Putative type II secretion system protein F (gspF). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Putative type II secretion system protein F (gspF), Recombinant Protein
Full Product Name
Recombinant Escherichia coli Putative type II secretion system protein F (gspF)
Product Gene Name
Product Synonym Gene Name
gspF; hofF, hopF
Product Synonym Names
Recombinant Putative type II secretion system protein F (gspF); Putative type II secretion system protein F; T2SS protein F; General secretion pathway protein F Protein transport protein HofF
Sequence
MNYRYRAMTQ DGQKLQGIID ANDERQARLR LREEGLFLLD IRPQKSSGVK TRRPRISHSE LTLFTRQLAT LSAAALPLEE SLAVIGQQSS NKRLGDVLNQ VRSAILEGHP LSDALQHFPT LFDSLYRTLV KAGEKSGLLA PVLEKLADYN ENRQKIRSKL IQSLIYPCML TTVAIGVVII LLTAVVPKIT EQFVHMKQQL PLSTRILLGL SDTLQRTGPT LLATVFIVAV GFWLWLKRGN NRHRFHAMLL RVALIGPLIC AINSARYLRT LSILQSSGVP LLDGMNLSTE SLNNLEIRQR LANAAENVRQ GNSIHLSLEQ TAIFPPMMLY MVASGEKSGQ LGTLMVRAAD NQETLQQNRI ALTLSIFEPA LIITMALIVL FIVVSVLQPL LQLNSMIN
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C. For extended storage, store at -20 or -80 degree C.
Molecular Weight
44,339 Da
Sequence Positions
1-398
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Chromosome Location
NC_000913.2 (3457839..3459035)
3D Structure
ModBase 3D Structure for P41441
Species
Escherichia coli (strain K12)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of gspF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for gspF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
general secretory pathway component, cryptic
NCBI Official Symbol
gspF
NCBI Official Synonym Symbols
ECK3314; hofF; hopF; JW3289
UniProt Protein Name
Putative type II secretion system protein F
UniProt Synonym Protein Names
General secretion pathway protein F; Protein transport protein HofF
UniProt Gene Name
gspF
UniProt Synonym Gene Names
hofF; hopF; T2SS protein F
UniProt Entry Name
GSPF_ECOLI
NCBI Protein Information
general secretory pathway component, cryptic

NCBI Summary for gspF

A divergon is a regulon comprised of two adjacent divergent operons or genes. [More information is available at EcoGene: EG12885]. In Escherichia coli, gspF is a member of an operon of genes (gspC-O) which are not normally expressed but are homologous to those encoding the secreton, or type II secretion machinery in Klebsiella oxytoca and Aeromonase hydrophila, among others . [More information is available at EcoCyc: G7705].
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UniProt Comments for gspF

Function: Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins

Potential.

Subcellular location: Cell inner membrane; Multi-pass membrane protein.

Induction: Silenced by the DNA-binding protein H-NS under standard growth conditions. Ref.5

Miscellaneous: Part of a cryptic operon that encodes proteins involved in type II secretion machinery in other organisms, but is not expressed in strain K12.

Sequence similarities: Belongs to the GSP F family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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