Drosophila Simulans DsimGD12816 Recombinant Protein – Cat# MBS1165262

Protein Wntless (Wls) (DsimGD12816) Drosophila Simulans Recombinant Protein

Name: DsimGD12816 Recombinant Protein; Drosophila Simulans; MyBioSource
Brand: MyBioSource
SKU: MBS1165262
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1165262

MBS1165262 is a recombinant protein targeting DsimGD12816. Also known as Recombinant Drosophila simulans Protein wntless (wls). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Protein wntless (wls) (DsimGD12816), Recombinant Protein

Full Product Name

Recombinant Drosophila simulans Protein wntless (wls)

Product Gene Name

DsimGD12816 recombinant protein

Product Synonym Gene Name

wls; GD12816

Product Synonym Names

Recombinant Protein wntless (wls); Protein wntless

Sequence

APLPAGHVTV LGSLCREDHA RQNDTSFLLY SRGAGACIPV TREEVEQDST KMANELVHVF QMPLPRDLRD LDYSRWQQNL IGVLQVEFGY DSSSELREPP RELQLTIDMR LAYRNKGDPD NGWKLYAHGV EHRYLDCVTS HVGPTETLYS CDMIPLFELG ALHHSFYLLN LRFPLDTPSQ MNLQFGHMHD LTLTAIHQNG GFTQIWLLLK TMLFPFVVGI MIWFWRRVHL LQRSPALLEY MLIYLGAALT FLNLPLEYLS LVYEMPYMLL LSDIRQGIFY AMLLTFWLVF AGEHMLIQDA PNKSTIRSRY WKHLSAVVVG CISLFVFDIC ERGVQLRNPF YSIWTTPLGA KVAMTFIVLA GVSAAIYFLF LCYMIWKVFR NIGDKRTSLP SMSQARRLHY EGLIYRFKFL MLATLVCAAL TVAGFIMGQM AEGQWDWNDN VAIQPTSAFL TGVYGMWNIY IFALLILYAP SHKQWPTMHH SDETTQSNEN IVASAASEEI EFSHLPSDSN PSEISSLTSF TRKVAFD

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

Molecular Weight

64,088 Da

Sequence Positions

36-562

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

Chromosome Location

Chromosome: 3L; NT_167068.1 (10555985..10558237, complement). Location: chromosome: 3L

3D Structure

ModBase 3D Structure for B4QPR1

Species

Drosophila simulans (Fruit fly)

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of DsimGD12816 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DsimGD12816. It may not necessarily be applicable to this product.

NCBI GI #

195589455

NCBI GeneID

6737635

NCBI Accession #

XP_002084467.1

UniProt Primary Accession #

B4QPR1

GenBank Nucleotide #

XM_002084431.1

NCBI Official Full Name

GD12816

NCBI Official Symbol

DsimGD12816

NCBI Official Synonym Symbols

dsim_GLEANR_12875; GD12816

UniProt Protein Name

Protein wntless

UniProt Gene Name

wls

UniProt Entry Name

WLS_DROSI

NCBI Protein Information

GD12816 gene product from transcript GD12816-RA; DsimGD12816-PA

UniProt Comments for DsimGD12816

Function: A segment polarity gene required for wingless (wg)-dependent patterning processes, acting in both wg-sending cells and wg-target cells. In non-neuronal cells wls directs wg secretion. The wls traffic loop encompasses the Golgi, the cell surface, an endocytic compartment and a retrograde route leading back to the Golgi, and involves clathrin-mediated endocytosis and the retromer complex (a conserved protein complex consisting of Vps35 and Vps26). In neuronal cells (the larval motorneuron NMJ), the wg signal moves across the synapse via the release of wls-containing exosome-like vesicles. Postsynaptic wls is required for the trafficking of fz2 through the fz2-interacting protein Grip

By similarity. UniProtKB Q95ST2

Subunit structure: Interacts with wg; in the Golgi. Interacts with Vps35, a component of the retromer complex; wls stability is regulated by Vps35

By similarity. UniProtKB Q95ST2

Subcellular location: Cell junction › synapse › presynaptic cell membrane; Multi-pass membrane protein

By similarity. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein

By similarity. Cell membrane; Multi-pass membrane protein

By similarity. Endoplasmic reticulum membrane; Multi-pass membrane protein

By similarity. Endosome membrane; Multi-pass membrane protein

By similarity. Golgi apparatus membrane; Multi-pass membrane protein

By similarity. Note: In non-neuronal cells, wls binds to wg in the Golgi and accompanies it to the plasma membrane where the two proteins dissociate. Wg is secreted and wls is then internalized and returns to the Golgi apparatus in a retromer-dependent manner. Wls and wg colocalize in the Golgi apparatus in wg-producing cells, and reduced expression is seen in non-producing cells. Endoplasmic recticulum expression is unchanged in wg-producing versus non-producing cells. In neuronal cells, wls is localized both pre- and postsynaptically and is transferred trans-synaptically from the pre- to the postsynaptic compartment

By similarity. UniProtKB Q95ST2

Sequence similarities: Belongs to the wntless family.

Sequence caution: The sequence EDX10052.1 differs from that shown. Reason: Erroneous gene model prediction.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.