3-Oxoacyl-[Acyl-Carrier-Protein] Synthase 2 (fabF) Escherichia Coli (Strain K12) Recombinant Protein

Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Has a preference for short chain acid substrates and may function to supply the octanoic substrates for lipoic acid biosynthesis.

The fabJ-encoded beta-ketoacyl- synthase IV from Escherichia coli is sensitive to cerulenin and specific for short-chain substrates.Siggaard-Andersen M., Wissenbach M., Chuck J.-A., Svendsen I., Olsen J.G., von Wettstein-Knowles P.V.Proc. Natl. Acad. Sci. U.S.A. 91:11027-11031(1994) The putative fabJ gene of Escherichia coli fatty acid synthesis is the fabF gene.Magnuson K., Carey M.R., Cronan J.E. Jr.J. Bacteriol. 177:3593-3595(1995) A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.DNA Res. 3:137-155(1996) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Altered molecular form of acyl carrier protein associated with beta-ketoacyl-acyl carrier protein synthase II (fabF) mutants.Jackowski S., Rock C.O.J. Bacteriol. 169:1469-1473(1987) Beta-ketoacyl-acyl carrier protein synthase II of Escherichia coli. Evidence for function in the thermal regulation of fatty acid synthesis.Garwin J.L., Klages A.L., Cronan J.E. Jr.J. Biol. Chem. 255:3263-3265(1980) Cloning of the fabF gene in an expression vector and in vitro characterization of recombinant fabF and fabB encoded enzymes from Escherichia coli.Edwards P., Nelsen J.S., Metz J.G., Dehesh K.FEBS Lett. 402:62-66(1997) Crystal structure of beta-ketoacyl-acyl carrier protein synthase II from E Coli reveals the molecular architecture of condensing enzymes.Huang W., Jia J., Edwards P., Dehesh K., Schneider G., Lindqvist Y.EMBO J. 17:1183-1191(1998) Structure of the complex between the antibiotic cerulenin and its target, beta-ketoacyl-acyl carrier protein synthase.Moche M., Schneider G., Edwards P., Dehesh K., Lindqvist Y.J. Biol. Chem. 274:6031-6034(1999)

Small volumes of fabF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1166991 is a recombinant protein targeting fabF. Also known as Recombinant Escherichia coli (strain K12) 3-oxoacyl-[acyl-carrier-protein] synthase 2. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.