RNA-Splicing Ligase Rtcb (rtcB) Escherichia Coli Recombinant Protein

RNA ligase that mediates the joining of broken tRNA-like st-loop structures in case of tRNA damage. Probably participates to tRNA restriction-repair by ligating broken tRNA-like st-loop structures with 2',3'-cyclic phosphate and 5'-OH ends to form a splice junction with a 2'-OH, 3',5'-phosphodiester, a step that requires GTP. Also acts as a DNA ligase in case of DNA damage by splicing 'dirty' DNA breaks, characterized by 3'-PO4 (or cyclic-PO4) and 5'-OH ends that cannot be sealed by classical DNA ligases.

The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Characterization of the Escherichia coli RNA 3'-terminal phosphate cyclase and its sigma54-regulated operon.Genschik P., Drabikowski K., Filipowicz W.J. Biol. Chem. 273:25516-25526(1998) RtcB is the RNA ligase component of an Escherichia coli RNA repair operon.Tanaka N., Shuman S.J. Biol. Chem. 286:7727-7731(2011) RtcB, a novel RNA ligase, can catalyze tRNA splicing and HAC1 mRNA splicing in vivo.Tanaka N., Meineke B., Shuman S.J. Biol. Chem. 286:30253-30257(2011) Novel mechanism of RNA repair by RtcB via sequential 2',3'-cyclic phosphodiesterase and 3'-Phosphate/5'-hydroxyl ligation reactions.Tanaka N., Chakravarty A.K., Maughan B., Shuman S.J. Biol. Chem. 286:43134-43143(2011) The sequential 2',3'-cyclic phosphodiesterase and 3'-phosphate/5'-OH ligation steps of the RtcB RNA splicing pathway are GTP-dependent.Chakravarty A.K., Shuman S.Nucleic Acids Res. 40:8558-8567(2012) RNA ligase RtcB splices 3'-phosphate and 5'-OH ends via covalent RtcB-(histidinyl) -GMP and polynucleotide-(3') pp(5') G intermediates.Chakravarty A.K., Subbotin R., Chait B.T., Shuman S.Proc. Natl. Acad. Sci. U.S.A. 109:6072-6077(2012) Rewriting the rules for end joining via enzymatic splicing of DNA 3'-PO4 and 5'-OH ends.Das U., Chakravarty A.K., Remus B.S., Shuman S.Proc. Natl. Acad. Sci. U.S.A. 110:20437-20442(2013)

Small volumes of rtcB recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1175065 is a recombinant protein targeting rtcB. Also known as Recombinant Escherichia coli RNA-splicing ligase RtcB. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.