E3 Ubiquitin-Protein Ligase RNF144B (RNF144B) Bovine Recombinant Protein

Function: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se

By similarity.

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Interacts with UBE2L3, UBE2L6 and LCMT2 as well as with BAX

By similarity.

Subcellular location: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note: Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation

By similarity.

Domain: The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. The transmembrane domain is essential for translocation to the mitochondria upon induction of apoptosis

By similarity.

Post-translational modification: Auto-ubiquitinated

By similarity.

Sequence similarities: Belongs to the RBR family. RNF144 subfamily.Contains 1 IBR-type zinc finger.Contains 2 RING-type zinc fingers.

Caution: Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.