Ba(e Coli-K12) DNA Ligase (ligA) Recombinant Protein

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli.Ishino Y., Shinagawa H., Makino K., Tsunasawa S., Sakiyama F., Nakata A.Mol. Gen. Genet. 204:1-7(1986) O'Connor M.J., Ally A., Ally D., Zhang X., Robichaud M., Backman K.Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.DNA Res. 4:91-113(1997) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Direct binding of FtsZ to ZipA, an essential component of the septal ring structure that mediates cell division in E. coli.Hale C.A., de Boer P.A.J.Cell 88:175-185(1997) Conserved residues in domain Ia are required for the reaction of Escherichia coli DNA ligase with NAD+.Sriskanda V., Shuman S.J. Biol. Chem. 277:9695-9700(2002) Structure-guided mutational analysis of the nucleotidyltransferase domain of Escherichia coli NAD+-dependent DNA ligase (LigA) .Zhu H., Shuman S.J. Biol. Chem. 280:12137-12144(2005) Structure-guided mutational analysis of the OB, HhH, and BRCT domains of Escherichia coli DNA ligase.Wang L.K., Nair P.A., Shuman S.J. Biol. Chem. 283:23343-23352(2008) Last stop on the road to repair structure of E. coli DNA ligase bound to nicked DNA-adenylate.Nandakumar J., Nair P.A., Shuman S.Mol. Cell 26:257-271(2007)

Small volumes of ligA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1184965 is a recombinant protein targeting ligA. Also known as Recombinant Ba(E Coli-k12) DNA ligase. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.