Transketolase 1 (tktA) Escherichia Coli (Strain K12) Recombinant Protein

Catalyzes the reversible transfer of a two-carbon ketol group from sedoheptulose-7-phosphate to glyceraldehyde-3-phosphate, producing xylulose-5-phosphate and ribose-5-phosphate. Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.

Nucleotide sequence of the Escherichia coli K-12 transketolase (tkt) gene.Sprenger G.A.Biochim. Biophys. Acta 1216:307-310(1993) Sprenger G.A.The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Escherichia coli K-12 a cooperatively developed annotation snapshot -- 2005.Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L.Nucleic Acids Res. 34:1-9(2006) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Analysis and sequence of the speB gene encoding agmatine ureohydrolase, a putrescine biosynthetic enzyme in Escherichia coli.Szumanski M.B.W., Boyle S.M.J. Bacteriol. 172:538-547(1990) Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y.Mol. Cell. Proteomics 8:215-225(2009) Transketolase A of Escherichia coli K12. Purification and properties of the enzyme from recombinant strains.Sprenger G.A., Schorken U., Sprenger G., Sahm H.Eur. J. Biochem. 230:525-532(1995) Crystal structure of Escherichia coli transketolase.Isupov M.N., Rupprecht M.P., Wilson K.S., Dauter Z., Littlechild J.A.Submitted (APR-1999) to the PDB data bankStrain and near attack conformers in enzymic thiamin catalysis X-ray crystallographic snapshots of bacterial transketolase in covalent complex with donor ketoses xylulose 5-phosphate and fructose 6-phosphate, and in noncovalent complex with acceptor aldose ribose 5-phosphate.Asztalos P., Parthier C., Golbik R., Kleinschmidt M., Hubner G., Weiss M.S., Friedemann R., Wille G., Tittmann K.Biochemistry 46:12037-12052(2007)

Small volumes of tktA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1185327 is a recombinant protein targeting tktA. Also known as Recombinant Escherichia coli (strain K12) Transketolase 1. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.