Mouse FHIT Recombinant Protein – Cat# MBS1190465

Bis(5'-Adenosyl)-Triphosphatase (FHIT) Mouse Recombinant Protein

Name: FHIT Recombinant Protein; Bis(5'-Adenosyl)-Triphosphatase; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS1190465
Price: 335 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1190465

Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extrely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to… More details

$335 / 0.02 mg (E-Coli) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Bis(5'-adenosyl)-triphosphatase (Fhit), Recombinant Protein

Full Product Name

Recombinant Mouse Bis(5'-adenosyl)-triphosphatase

Product Gene Name

Fhit recombinant protein

Product Synonym Names

AP3A hydrolase; AP3; Aase; Diadenosine 5',5'''-P1,P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein

Sequence

SFRFGQHLIK PSVVFLKTEL SFALVNRKPV VPGHVLVCPL RPVERFRDLH PDEVADLFQV TQRVGTVVEK HFQGTSITFS MQDGPEAGQT VKHVHVHVLP RKAGDFPRND NIYDELQKHD REEEDSPAFW RSEKEMAAEA EALRVYFQA

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

Molecular Weight

19.1 kDa

Sequence Positions

2-150aa; Full Length

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

3D Structure

ModBase 3D Structure for O89106

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Product References and Citations for Fhit recombinant protein

The murine Fhit locus isolation, characterization, and expression in normal and tumor cells.Pekarsky Y., Druck T., Cotticelli M.G., Ohta M., Shou J., Mendrola J., Montgomery J.C., Buchberg A.M., Siracusa L.D., Manenti G., Fong L.Y., Dragani T.A., Croce C.M., Huebner K.Cancer Res. 58:3401-3408(1998) Nitrilase and Fhit homologs are encoded as fusion proteins in Drosophila melanogaster and Caenorhabditis elegans.Pekarsky Y., Campiglio M., Siprashvili Z., Druck T., Sedkov Y., Tillib S., Draganescu A., Wermuth P., Rothman J.H., Huebner K., Buchberg A.M., Mazo A., Brenner C., Croce C.M.Proc. Natl. Acad. Sci. U.S.A. 95:8744-8749(1998) Muir-Torre-like syndrome in Fhit-deficient mice.Fong L.Y., Fidanza V., Zanesi N., Lock L.F., Siracusa L.D., Mancini R., Siprashvili Z., Ottey M., Martin S.E., Druck T., McCue P.A., Croce C.M., Huebner K.Proc. Natl. Acad. Sci. U.S.A. 97:4742-4747(2000) The tumor spectrum in FHIT-deficient mice.Zanesi N., Fidanza V., Fong L.Y., Mancini R., Druck T., Valtieri M., Rudiger T., McCue P.A., Croce C.M., Huebner K.Proc. Natl. Acad. Sci. U.S.A. 98:10250-10255(2001)

Other Notes

Small volumes of Fhit recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Fhit purchase

MBS1190465 is a recombinant protein targeting Fhit. Also known as Recombinant Mouse Bis(5'-adenosyl)-triphosphatase. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for Fhit. It may not necessarily be applicable to this product.

NCBI GI #

815930058

NCBI GeneID

14198

NCBI Accession #

NP_001295215.1

UniProt Primary Accession #

O89106

UniProt Secondary Accession #

Q6URW5; Q91VL1

UniProt Related Accession #

O89106

GenBank Nucleotide #

NM_001308286.1

NCBI Official Full Name

bis(5'-adenosyl)-triphosphatase isoform 2

NCBI Official Synonym Full Names

fragile histidine triad gene

NCBI Official Symbol

Fhit

NCBI Official Synonym Symbols

Fra14A2; AW045638

UniProt Protein Name

Bis(5'-adenosyl)-triphosphatase

UniProt Synonym Protein Names

AP3A hydrolase; AP3Aase; Diadenosine 5',5'''-P1,P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein

Protein Family

Bifunctional bis(5'-adenosyl)-triphosphatase/adenylylsulfatase

UniProt Gene Name

Fhit

UniProt Synonym Gene Names

AP3Aase

UniProt Entry Name

FHIT_MOUSE

NCBI Protein Information

bis(5'-adenosyl)-triphosphatase

NCBI Summary for Fhit

This gene encodes a member of the HIT family of proteins that are characterized by the presence of a histidine triad sequence. The encoded protein is a diadenosine triphosphate hydrolase enzyme that cleaves the P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. This locus is very fragile and has been found to be altered in different types of cancers. Mice lacking the encoded protein display increased susceptibility to spontaneous and induced tumors. Ectopic expression of the encoded protein in such knockout mice inhibits tumor development. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

UniProt Comments for Fhit

FHIT: a member of the histidine triad gene family. A diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. Its gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. A possible tumor suppressor in specific tissues. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines. Aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas.

Protein type: Motility/polarity/chemotaxis; Tumor suppressor; Hydrolase; DNA replication; EC 3.6.1.29; Nucleotide Metabolism - purine

Cellular Component: cytoplasm; cytosol; intracellular; nucleus; plasma membrane

Molecular Function: bis(5'-adenosyl)-triphosphatase activity; catalytic activity; hydrolase activity; identical protein binding; nickel ion binding; nucleotide binding; ubiquitin protein ligase binding

Biological Process: apoptosis; diadenosine triphosphate catabolic process; DNA replication; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; nucleotide metabolic process; purine nucleotide metabolic process; regulation of transcription, DNA-dependent; transcription, DNA-dependent

Research Articles on Fhit

1. Fhit deficiency-induced global genome instability promotes mutation and clonal expansion

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Fhit recombinant protein

Reference Product	PubMed Publications
Jun recombinant protein	>109 publications with Fhit and Jun
Rassf1 recombinant protein	>36 publications with Fhit and Rassf1
Src recombinant protein	>9 publications with Fhit and Src
Cdkn1a recombinant protein	>6 publications with Fhit and Cdkn1a
Dnmt3b recombinant protein	>5 publications with Fhit and Dnmt3b
Akt1 recombinant protein	>2 publications with Fhit and Akt1

Pathways associated with Fhit recombinant protein

Products by Pathway	Pathway Diagram
Non-small Cell Lung Cancer Pathway antibodies	Non-small Cell Lung Cancer Pathway Diagram
Non-small Cell Lung Cancer Pathway antibodies	Non-small Cell Lung Cancer Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram
Small Cell Lung Cancer Pathway antibodies	Small Cell Lung Cancer Pathway Diagram
Small Cell Lung Cancer Pathway antibodies	Small Cell Lung Cancer Pathway Diagram

Diseases associated with Fhit recombinant protein

Disease Name	Pubmed Publications
Carcinoma Antibodies	>548 publications with Fhit and Carcinoma
Lung Neoplasms Antibodies	>196 publications with Fhit and Lung Neoplasms
Carcinoma, Squamous Cell Antibodies	>180 publications with Fhit and Carcinoma, Squamous Cell
Lung Diseases Antibodies	>165 publications with Fhit and Lung Diseases
Carcinoma, Non-Small-Cell Lung Antibodies	>100 publications with Fhit and Carcinoma, Non-Small-Cell Lung
Breast Neoplasms Antibodies	>97 publications with Fhit and Breast Neoplasms
Neoplasm Metastasis Antibodies	>92 publications with Fhit and Neoplasm Metastasis
Stomach Neoplasms Antibodies	>70 publications with Fhit and Stomach Neoplasms
Uterine Cervical Neoplasms Antibodies	>70 publications with Fhit and Uterine Cervical Neoplasms
Neoplasms, Experimental Antibodies	>70 publications with Fhit and Neoplasms, Experimental

Organs/Tissues associated with Fhit recombinant protein

Organ/Tissue Name	Pubmed Publications
Mixed Antibodies	>12 publications with Fhit and Mixed