Carbon Storage Regulator (csrA) Escherichia Coli Recombinant Protein

CsrA translationally represses the GGDEF diguanylate cyclase enzymes YdeH and YcdT, which are needed for normal motility (Jonas, 2008). [More information is available at EcoGene: EG11447]. CsrA is a regulator of carbohydrate metabolism that affects glycogen biosynthesis , glycogen degradation , gluconeogenesis , and glycolysis . [More information is available at EcoCyc: EG11447].

A key translational regulator that binds mRNA to regulate translation initiation and/or mRNA stability, initially identified for its effects on central carbon metabolism (PubMed:8393005). Mediates global changes in gene expression, shifting from rapid growth to stress survival by linking envelope stress, the stringent response and the catabolite repression systems (PubMed:21488981, PubMed:28924029). Binds to the 5'-UTR of mRNA to repress or activate translation; 2 binding sites on the homodimer can bridge 2 sites within target RNA (). Exerts reciprocal effects on enzymes of gluconeogenesis and glycogen biosynthesis versus those of glycolysis (PubMed:7493933, PubMed:16923806). Negatively effects glycogen biosynthesis, gluconeogenesis, alters cell size and surface properties (PubMed:8393005, PubMed:7751274, PubMed:7493933). Activates regulates expression of glycolysis genes (PubMed:7493933). Represses biofilm formation (PubMed:11741870). Regulates glycogen synthesis under both aerobic and anaerobic conditions; overexpression strongly inhibits glycogen accumulation (PubMed:8393005). Binds to 4 sites in its own promoter, including the Shine-Dalgarno sequence, repressing its own translation; mutating the binding-sites decreases repression (PubMed:21696456). Indirectly activates transcription from 1 of its 5 promoters, which is responsible for increased expression during stationary phase (PubMed:21696456). Binds to at least 720 transcripts in strain K12 / CF7789, many of which are also part of the stringent response, including relA, spoT and dksA; slightly represses RelA and slightly activates DskA translation (PubMed:21488981). Binds to and represses the ECF sigma factor rpoE promoter (PubMed:28924029). Accelerates the degradation of glgC gene transcripts; overexpression further decreases glgC transcripts (PubMed:7751274). Binds 2 sites in the glgC mRNA leader, 1 of which overlaps the Shine-Dalgarno sequence, preventing ribosome-binding and thus destabilizing the mRNA (PubMed:12067347). Acts to inhibit interaction between the CcdB (also known as LetD) protein and the A subunit of DNA gyrase (PubMed:8604133). Required to activate motility and flagellum biosynthesis through the post-transcriptional activation of flhDC expression by binding to and stabilizing the flhDC message (PubMed:11298291). Represses translation of iraD mRNA via translational coupling to an upstream open reading frame (PubMed:28851853). Binds to mRNA and reduces levels of probable diguanylate cyclases dgcT and dgcZ (PubMed:18713317).