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CBFA2T2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS120008

In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CBFA2T2, Monoclonal Antibody
Full Product Name
Mouse monoclonal antibody Anti-Human CBFA2T2
Product Gene Name
anti-CBFA2T2 antibody
Product Synonym Names
core-binding factor,alpha subunit 2; MTGR1; EHT; ZMYND3
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CBF5I134
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Molecular Weight
65,878 Da
OMIM
603672
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Applications Tested/Suitable for anti-CBFA2T2 antibody
Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS)

Quality Control of anti-CBFA2T2 antibody

Western blot analysis of immunized recombinant protein, using anti-CBFA2T2 monoclonal antibody.
Mouse Anti-CBFA2T2 Monoclonal Antibody – Cat# MBS120008

Quality Control #2 of anti-CBFA2T2 antibody

Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Mouse Anti-CBFA2T2 Monoclonal Antibody, Quality Control #2 – Cat# MBS120008

Western Blot (WB) of anti-CBFA2T2 antibody

Detection of human CBFA2T2 by Western blot.

Samples: Whole cell lysate (25 ug) from HeLa cells. [Lot No. CBF5I134-2]

Predicted molecular weight: 67 kDa
Mouse Anti-CBFA2T2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS120008

Flow Cytometry (FC/FACS) of anti-CBFA2T2 antibody

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-CBFA2T2 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. CBF5I134-2]
Mouse Anti-CBFA2T2 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS120008

Immunocytochemistry (ICC) of anti-CBFA2T2 antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti- CBFA2T2 mAb. [Lot No. CBF5I134-2]
Mouse Anti-CBFA2T2 Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS120008

Other Notes

Small volumes of anti-CBFA2T2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CBFA2T2 purchase

MBS120008 is an antibody targeting CBFA2T2. Also known as Mouse monoclonal antibody Anti-Human CBFA2T2. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CBFA2T2. It may not necessarily be applicable to this product.

NCBI GI #
34193058
NCBI GeneID
9139
NCBI Accession #
BC040344
UniProt Secondary Accession #
Q5TGE4; Q5TGE5; Q5TGE6; Q5TGE7; Q8IWF3; Q96B06; Q96L00; Q9H436; Q9UJP8; B2RAE6; F8W6D7
UniProt Related Accession #
O43439
NCBI Official Full Name
Homo sapiens core-binding factor, runt domain, alpha subunit 2; translocated to, 2, mRNA
NCBI Official Synonym Full Names
core-binding factor, runt domain, alpha subunit 2; translocated to, 2
NCBI Official Symbol
CBFA2T2
NCBI Official Synonym Symbols
EHT; p85; MTGR1; ZMYND3
UniProt Protein Name
Protein CBFA2T2
UniProt Synonym Protein Names
ETO homologous on chromosome 20; MTG8-like protein; MTG8-related protein 1; Myeloid translocation-related protein 1; p85
Protein Family
Protein
UniProt Gene Name
CBFA2T2
UniProt Entry Name
MTG8R_HUMAN
NCBI Protein Information
protein CBFA2T2; ETO homolog on chromosome 20; ETO homologous on chromosome 20; MTG8-like protein; MTG8-related protein 1; myeloid translocation gene-related protein 1; myeloid translocation-related protein 1

UniProt Comments for CBFA2T2

CBFA2T2: May function as a complex with the chimeric protein RUNX1/AML1-CBFA2T1/MTG8 which is produced in acute myeloid leukemia with the chromosomal translocation t(8;21). May thus be involved in the repression of AML1-dependent transcription and the induction of G-CSF/CSF3-dependent cell growth. May be a tumor suppressor gene candidate involved in myeloid tumors with the deletion of the 20q11 region. Belongs to the CBFA2T family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 20q11

Cellular Component: nucleus

Molecular Function: protein binding; metal ion binding; transcription corepressor activity; transcription factor activity

Biological Process: epithelial cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
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Research Articles on CBFA2T2

1. The MTGR1 gene depends on a GC-box-rich sequence for transcriptional regulation and possible ubiquitous expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-CBFA2T2 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >2 publications with CBFA2T2 and Disease Models, Animal
 Immune System Diseases Antibodies  >1 publications with CBFA2T2 and Immune System Diseases
 Inflammation Antibodies  >1 publications with CBFA2T2 and Inflammation

Organs/Tissues associated with anti-CBFA2T2 antibody

 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >5 publications with CBFA2T2 and Intestine
 Embryonic Tissue Antibodies  >4 publications with CBFA2T2 and Embryonic Tissue
 Bone Antibodies  >3 publications with CBFA2T2 and Bone
 Nerve Antibodies  >3 publications with CBFA2T2 and Nerve
 Blood Antibodies  >2 publications with CBFA2T2 and Blood
 Thymus Antibodies  >1 publications with CBFA2T2 and Thymus
 Kidney Antibodies  >1 publications with CBFA2T2 and Kidney
 Placenta Antibodies  >1 publications with CBFA2T2 and Placenta
 Uncharacterized Tissue Antibodies  >1 publications with CBFA2T2 and Uncharacterized Tissue
 Vascular Antibodies  >1 publications with CBFA2T2 and Vascular
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