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GTF2IRD1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS120021

The protein encoded by this gene contains five GTF2I-like repeats and each repeat possesses a potential helix-loop-helix (HLH) motif. It may have the ability to interact with other HLH-proteins and function as a transcription factor or as a positive… More details

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Product Overview

Online Datasheet in PDF
Product Name
GTF2IRD1, Monoclonal Antibody
Full Product Name
Mouse monoclonal antibody Anti-Human GTF2IRD1
Product Gene Name
anti-GTF2IRD1 antibody
Product Synonym Names
GTF2I repeat domain containing 1 (GTF2IRD1); GTF3; RBAP2; CREAM1; MUSTRD1; WBSCR11; WBSCR12; hMusTRD1alpha1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
GTF5I102
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Molecular Weight
107,979 Da
OMIM
604318
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Applications Tested/Suitable for anti-GTF2IRD1 antibody
ELISA (EIA), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB)

Quality Control of anti-GTF2IRD1 antibody

Western blot analysis of immunized recombinant protein, using anti-GTF2IRD1 monoclonal antibody.
Mouse Anti-GTF2IRD1 Monoclonal Antibody – Cat# MBS120021

Quality Control #2 of anti-GTF2IRD1 antibody

Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Mouse Anti-GTF2IRD1 Monoclonal Antibody, Quality Control #2 – Cat# MBS120021

Western Blot (WB) of anti-GTF2IRD1 antibody

Detection of human GTF2IRD1 by Western blot.

Sample: Whole cell lysate was obtained from HeLa cells transiently transfected with vector (lanes 1, 3) or myc-tagged human GTF2IRD1 (lanes 2, 4). Primary antibodies: anti-myc tag (1 ug/ml) (lanes 1, 2) or ant

Predicted molecular weight: 106 kDa
Mouse Anti-GTF2IRD1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS120021

Immunoprecipitation (IP) of anti-GTF2IRD1 antibody

Immunoprecipitation: RIPA lysate of HeLa cells transfected with vector (lanes 1, 3) or myc-tagged GTF2IRD1 (lanes 2, 4) was incubated with anti-GTF2IRD1 (BMR00190) conjugated with protein G sepharose. Immunocomplex was analyzed by Western blot with anti-m

Predicted molecular weight: 106 kDa
Mouse Anti-GTF2IRD1 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS120021

Immunocytochemistry (ICC) of anti-GTF2IRD1 antibody

Localization of human GTF2IRD1 in HeLa cells.

HeLa cells transiently transfected with the myc-tagged GTF2IRD1 expression vector were fixed and stained with anti- GTF2IRD1 (BMR00190) and Hoechst 33342. Human GTF2IRD1 fluorescence signals were observed exc
Mouse Anti-GTF2IRD1 Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS120021

Other Notes

Small volumes of anti-GTF2IRD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GTF2IRD1 purchase

MBS120021 is an antibody targeting GTF2IRD1. Also known as Mouse monoclonal antibody Anti-Human GTF2IRD1. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GTF2IRD1. It may not necessarily be applicable to this product.

NCBI GI #
312836809
NCBI GeneID
9569
NCBI Accession #
NP_057412.1
UniProt Secondary Accession #
O95444; Q6DSU6; Q75MX7; Q86UM3; Q8WVC4; Q9UHK8; Q9UI91
UniProt Related Accession #
Q9UHL9
GenBank Nucleotide #
NM_016328
NCBI Official Full Name
Homo sapiens GTF2I repeat domain containing 1 (GTF2IRD1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
GTF2I repeat domain containing 1
NCBI Official Symbol
GTF2IRD1
NCBI Official Synonym Symbols
BEN; WBS; GTF3; RBAP2; CREAM1; MUSTRD1; WBSCR11; WBSCR12; hMusTRD1alpha1
UniProt Protein Name
General transcription factor II-I repeat domain-containing protein 1
UniProt Synonym Protein Names
General transcription factor III; MusTRD1/BEN; Muscle TFII-I repeat domain-containing protein 1; Slow-muscle-fiber enhancer-binding protein; USE B1-binding protein; Williams-Beuren syndrome chromosomal region 11 protein; Williams-Beuren syndrome chromosomal region 12 protein
Protein Family
General transcription factor II-I repeat domain-containing protein
UniProt Gene Name
GTF2IRD1
UniProt Entry Name
GT2D1_HUMAN
NCBI Protein Information
general transcription factor II-I repeat domain-containing protein 1; USE B1-binding protein; Williams-Beuren syndrome chromosome region 11; binding factor for early enhancer; general transcription factor 3; general transcription factor III; muscle TFII-I repeat domain-containing protein 1 alpha 1; slow-muscle-fiber enhancer-binding protein; williams-Beuren syndrome chromosomal region 12 protein

NCBI Summary for GTF2IRD1

The protein encoded by this gene contains five GTF2I-like repeats and each repeat possesses a potential helix-loop-helix (HLH) motif. It may have the ability to interact with other HLH-proteins and function as a transcription factor or as a positive transcriptional regulator under the control of Retinoblastoma protein. This gene plays a role in craniofacial and cognitive development and mutations have been associated with Williams-Beuren syndrome, a multisystem developmental disorder caused by deletion of multiple genes at 7q11.23. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]
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UniProt Comments for GTF2IRD1

GTF2IRD1: May be a transcription regulator involved in cell-cycle progression and skeletal muscle differentiation. May repress GTF2I transcriptional functions, by preventing its nuclear residency, or by inhibiting its transcriptional activation. May contribute to slow-twitch fiber type specificity during myogenesis and in regenerating muscles. Binds troponin I slow-muscle fiber enhancer (USE B1). Binds specifically and with high affinity to the EFG sequences derived from the early enhancer of HOXC8. GTF2IRD1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2IRD1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. Belongs to the TFII-I family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; multicellular organismal development; transition between slow and fast fiber

Disease: Williams-beuren Syndrome
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Research Articles on GTF2IRD1

1. GTF2IRD1 is SUMOylated by the SUMO E2 ligase UBC9 and the level of SUMOylation is enhanced by PIASxbeta.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GTF2IRD1 antibody

 Reference Product  PubMed Publications
 SMAD3 antibody  >1 publications with GTF2IRD1 and SMAD3
 PIAS2 antibody  >1 publications with GTF2IRD1 and PIAS2

Pathways associated with anti-GTF2IRD1 antibody

 Products by Pathway  Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 CGMP-PKG Signaling Pathway antibodies  CGMP-PKG Signaling Pathway Diagram
 CGMP-PKG Signaling Pathway antibodies  CGMP-PKG Signaling Pathway Diagram

Diseases associated with anti-GTF2IRD1 antibody

 Disease Name  Pubmed Publications
 Williams Syndrome Antibodies  >48 publications with GTF2IRD1 and Williams Syndrome
 Heart Diseases Antibodies  >34 publications with GTF2IRD1 and Heart Diseases
 Neurobehavioral Manifestations Antibodies  >33 publications with GTF2IRD1 and Neurobehavioral Manifestations
 Craniofacial Abnormalities Antibodies  >12 publications with GTF2IRD1 and Craniofacial Abnormalities
 Mental Disorders Antibodies  >6 publications with GTF2IRD1 and Mental Disorders
 Neural Tube Defects Antibodies  >1 publications with GTF2IRD1 and Neural Tube Defects
 Heart Defects, Congenital Antibodies  >1 publications with GTF2IRD1 and Heart Defects, Congenital
 Hyperplasia Antibodies  >1 publications with GTF2IRD1 and Hyperplasia

Organs/Tissues associated with anti-GTF2IRD1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >43 publications with GTF2IRD1 and Muscle
 Brain Antibodies  >17 publications with GTF2IRD1 and Brain
 Embryonic Tissue Antibodies  >7 publications with GTF2IRD1 and Embryonic Tissue
 Vascular Antibodies  >3 publications with GTF2IRD1 and Vascular
 Eye Antibodies  >3 publications with GTF2IRD1 and Eye
 Connective Tissue Antibodies  >2 publications with GTF2IRD1 and Connective Tissue
 Mouth Antibodies  >1 publications with GTF2IRD1 and Mouth
 Testis Antibodies  >1 publications with GTF2IRD1 and Testis
 Liver Antibodies  >1 publications with GTF2IRD1 and Liver
 Bone Antibodies  >1 publications with GTF2IRD1 and Bone
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