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NARS Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS120033

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NARS, Monoclonal Antibody
Full Product Name
Mouse monoclonal antibody Anti-Human NARS
Product Gene Name
anti-NARS antibody
Product Synonym Names
asparaginyl-tRNA synthetase; ASNRS
Clonality
Monoclonal
Isotype
IgG1
Clone Number
NARS2G5
Host
Mouse
Species Reactivity
Human
Form/Format
Mouse monoclonal anti-human NARS antibody in PBS(3.0 mM KCI, 1.5 mM KH2HPO4140 mM NaCI,8.0 mM Na2HPO4(pH 7.4) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
Preparation and Storage
Store at 2-8°C for up to one year.
We recommend storing at -20°C for long term storage.
Avoid repeat freezing and thawing cycles.
OMIM
108410
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Immunogen
Recombinant
Sterility
Filtered through a 0.22 um membrane.
Disposal
This antibody solution contains sodium azide (NaN3) as a preservatve. There is a poteintial hazard that NaN3 reacts with coppler or lead to produce and explosive compound. For safe disposal, the vial has to be washed thoroughly with water.
Safety Warnings and precautions
Caution must be taken to avoid contact with skin or eyes . In such a case, rinse thoughly at once with water. Do not ingest, inhale , or swallow. Seek medical attention inmediately. Wear appropiate protective clothing such as laboratory overalls, safety glass and gloves. It is strongly advised that this product should be handled by people who have bee well trained in laboratory tecniques and that it is handled with care pursuant to the principles of good laboratory practice . All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off.
Application Notes for anti-NARS antibody
WB: 0.2-2 ug/mL
IP: 100-500 ug/ sample
ICC: 2-100 ug/mL

Quality Control of anti-NARS antibody

Western blot analysis of immunized recombinant protein, using anti-NARS monoclonal antibody.
Mouse Anti-NARS Monoclonal Antibody – Cat# MBS120033

Quality Control #2 of anti-NARS antibody

Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Mouse Anti-NARS Monoclonal Antibody, Quality Control #2 – Cat# MBS120033

Western Blot (WB) of anti-NARS antibody

Detection of NARS by Western blot.

Samples: Whole cell lysate from human HT1080 (H, 25 ug) and mouse NIH3T3 (M, 25 ug) cells. [Lot No. NARS2G5-3]

Predicted molecular weight: 62 kDa
Mouse Anti-NARS Monoclonal Antibody, Western Blot (WB) – Cat# MBS120033

Immunoprecipitation (IP) of anti-NARS antibody

Immunoprecipitation: RIPA lysate of HT1080 cells was incubated with anti-NARS mAb. [Lot No. NARS2G5-1]

Predicted molecular weight: 62 kDa
Mouse Anti-NARS Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS120033

Immunocytochemistry (ICC) of anti-NARS antibody

Immunostaining analysis in HT1080 cells. HT1080 cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-NARS mAb. [Lot No. NARS2G5-1]
Mouse Anti-NARS Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS120033

Other Notes

Small volumes of anti-NARS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NARS purchase

MBS120033 is an antibody targeting NARS. Also known as Mouse monoclonal antibody Anti-Human NARS. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NARS. It may not necessarily be applicable to this product.

NCBI GI #
12804548
NCBI GeneID
4677
NCBI Accession #
BC001687
UniProt Secondary Accession #
Q53GU6; B4DG16
UniProt Related Accession #
O43776
NCBI Official Full Name
Homo sapiens asparaginyl-tRNA synthetase, mRNA
NCBI Official Synonym Full Names
asparaginyl-tRNA synthetase
NCBI Official Symbol
NARS
NCBI Official Synonym Symbols
ASNRS; NARS1
UniProt Protein Name
Asparagine--tRNA ligase, cytoplasmic
UniProt Synonym Protein Names
Asparaginyl-tRNA synthetase; AsnRS
Protein Family
Probable sensor histidine kinase
UniProt Gene Name
NARS
UniProt Entry Name
SYNC_HUMAN
NCBI Protein Information
asparagine--tRNA ligase, cytoplasmic; asparagine tRNA ligase 1, cytoplasmic; asparaginyl-tRNA synthetase, cytoplasmic

UniProt Comments for NARS

NARS: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases. [provided by RefSeq, Jul 2008]

Protein type: Ligase; Translation; EC 6.1.1.22

Chromosomal Location of Human Ortholog: 18q21.31

Cellular Component: mitochondrion; cytoplasm; cytosol

Molecular Function: nucleic acid binding; asparagine-tRNA ligase activity; ATP binding

Biological Process: asparaginyl-tRNA aminoacylation; tRNA aminoacylation for protein translation; gene expression
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Research Articles on NARS

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NARS antibody

 Reference Product  PubMed Publications
 QARS antibody  >1 publications with NARS and QARS
 AARS antibody  >1 publications with NARS and AARS

Pathways associated with anti-NARS antibody

 Products by Pathway  Pathway Diagram
 Aminoacyl-tRNA Biosynthesis Pathway antibodies  Aminoacyl-tRNA Biosynthesis Pathway Diagram
 Aminoacyl-tRNA Biosynthesis Pathway antibodies  Aminoacyl-tRNA Biosynthesis Pathway Diagram
 Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway antibodies  Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway Diagram
 Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway antibodies  Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway Diagram
 Cytosolic TRNA Aminoacylation Pathway antibodies  Cytosolic TRNA Aminoacylation Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 TRNA Aminoacylation Pathway antibodies  TRNA Aminoacylation Pathway Diagram

Diseases associated with anti-NARS antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >6 publications with NARS and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >5 publications with NARS and Cardiovascular Diseases
 Brain Diseases Antibodies  >5 publications with NARS and Brain Diseases
 Heart Diseases Antibodies  >4 publications with NARS and Heart Diseases
 Chromosome Aberrations Antibodies  >3 publications with NARS and Chromosome Aberrations
 Inflammation Antibodies  >3 publications with NARS and Inflammation
 Hemorrhage Antibodies  >3 publications with NARS and Hemorrhage
 Neoplasms, Experimental Antibodies  >2 publications with NARS and Neoplasms, Experimental
 Proteinuria Antibodies  >2 publications with NARS and Proteinuria
 Fatty Liver Antibodies  >2 publications with NARS and Fatty Liver

Organs/Tissues associated with anti-NARS antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >33 publications with NARS and Blood
 Liver Antibodies  >18 publications with NARS and Liver
 Brain Antibodies  >8 publications with NARS and Brain
 Bone Antibodies  >7 publications with NARS and Bone
 Connective Tissue Antibodies  >5 publications with NARS and Connective Tissue
 Vascular Antibodies  >5 publications with NARS and Vascular
 Intestine Antibodies  >4 publications with NARS and Intestine
 Lung Antibodies  >3 publications with NARS and Lung
 Skin Antibodies  >3 publications with NARS and Skin
 Umbilical Cord Antibodies  >3 publications with NARS and Umbilical Cord
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