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TCERG1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS120042

This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing… More details

$575 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TCERG1, Monoclonal Antibody
Full Product Name
Mouse monoclonal antibody Anti-Human TCERG1
Product Gene Name
anti-TCERG1 antibody
Product Synonym Names
transcription elongation regulator 1 (TCERG1); CA150; TAF2S; MGC133200
Clonality
Monoclonal
Isotype
IgG1
Clone Number
TCEAD79A
Host
Mouse
Species Reactivity
Human
Form/Format
Mouse monoclonal anti-human TCERG1 antibody in PBS (3.0 mM KCl, 1.5 mM KH PO , 140 mM NaCl, 8.0 mM Na HPO (pH 7.4)) containing 1% bovine serum albumin (BSA)and 0.05% sodium azide (NaN ).
Concentration
100 ug/ml (1.0 ml) (lot specific)
Preparation and Storage
Store at 2-8 C for up to one year. We recommend storing at –20 C for long-term storage. Avoid repeat freezing and thawing cycles.
Molecular Weight
121,690 Da
OMIM
605409
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Disposal
This antibody solution contains sodium azide (NaN3) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water.
Safety warnings and precautions
Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off.
Applications Tested/Suitable for anti-TCERG1 antibody
ELISA (EIA), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-TCERG1 antibody
WB: 0.2-2 ug/ml
FC: 0.5-2 ug/sample
ICC: 2-100 ug/ml

Quality Control of anti-TCERG1 antibody

Western blot analysis of immunized recombinant protein, using anti-TCERG1 monoclonal antibody.
Mouse Anti-TCERG1 Monoclonal Antibody – Cat# MBS120042

Quality Control #2 of anti-TCERG1 antibody

Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Mouse Anti-TCERG1 Monoclonal Antibody, Quality Control #2 – Cat# MBS120042

Western Blot (WB) of anti-TCERG1 antibody

Detection of TCERG1 by Western blot.

Samples: Whole cell lysate from human A2058 (H, 50 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. TCEAD79A-2]

Predicted molecular weight: 123 kDa
Mouse Anti-TCERG1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS120042

Flow Cytometry (FC/FACS) of anti-TCERG1 antibody

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TCERG1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. TCEAD79A-2]
Mouse Anti-TCERG1 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS120042

Immunocytochemistry (ICC) of anti-TCERG1 antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TCERG1 mAb. [Lot No. TCEAD79A-1]
Mouse Anti-TCERG1 Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS120042

Other Notes

Small volumes of anti-TCERG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TCERG1 purchase

MBS120042 is an antibody targeting TCERG1. Also known as Mouse monoclonal antibody Anti-Human TCERG1. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TCERG1. It may not necessarily be applicable to this product.

NCBI GI #
91208416
NCBI GeneID
10915
NCBI Accession #
NP_006697.2
UniProt Secondary Accession #
Q2NKN2; Q59EA1
UniProt Related Accession #
O14776
GenBank Nucleotide #
NM_006706
NCBI Official Full Name
Homo sapiens transcription elongation regulator 1 (TCERG1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
transcription elongation regulator 1
NCBI Official Symbol
TCERG1
NCBI Official Synonym Symbols
Urn1; CA150; TAF2S
UniProt Protein Name
Transcription elongation regulator 1
UniProt Synonym Protein Names
TATA box-binding protein-associated factor 2S; Transcription factor CA150
Protein Family
Transcription elongation regulator
UniProt Gene Name
TCERG1
UniProt Entry Name
TCRG1_HUMAN
NCBI Protein Information
transcription elongation regulator 1; TATA box binding protein (TBP)-associated factor, RNA polymerase II, S, 150kD; TATA box-binding protein-associated factor 2S; co-activator of 150 kDa; transcription factor CA150

UniProt Comments for TCERG1

TCERG1: Transcription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner. Necessary for TAT-dependent activation of the human immunodeficiency virus type 1 (HIV-1) promoter. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Spliceosome; Transcription factor

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleolus; nucleus; actin cytoskeleton

Molecular Function: protein binding; transcription coactivator activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter
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Research Articles on TCERG1

1. This study reveals that TCERG1 regulates HIV-1 transcriptional elongation by increasing the elongation rate of RNAPII and phosphorylation of Ser 2 within the carboxyl-terminal domain.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TCERG1 antibody

 Reference Product  PubMed Publications
 SF1 antibody  >6 publications with TCERG1 and SF1
 HTT antibody  >4 publications with TCERG1 and HTT

Pathways associated with anti-TCERG1 antibody

 Products by Pathway  Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram

Diseases associated with anti-TCERG1 antibody

 Disease Name  Pubmed Publications
 Cognition Disorders Antibodies  >4 publications with TCERG1 and Cognition Disorders
 Liver Diseases Antibodies  >2 publications with TCERG1 and Liver Diseases
 Adenocarcinoma Antibodies  >1 publications with TCERG1 and Adenocarcinoma
 Ataxia Antibodies  >1 publications with TCERG1 and Ataxia
 Inflammation Antibodies  >1 publications with TCERG1 and Inflammation
 Liver Neoplasms Antibodies  >1 publications with TCERG1 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >1 publications with TCERG1 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-TCERG1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4 publications with TCERG1 and Blood
 Liver Antibodies  >3 publications with TCERG1 and Liver
 Brain Antibodies  >2 publications with TCERG1 and Brain
 Bone Marrow Antibodies  >1 publications with TCERG1 and Bone Marrow
 Bone Antibodies  >1 publications with TCERG1 and Bone
 Salivary Gland Antibodies  >1 publications with TCERG1 and Salivary Gland
 Spleen Antibodies  >1 publications with TCERG1 and Spleen
 Mouth Antibodies  >1 publications with TCERG1 and Mouth
 Intestine Antibodies  >1 publications with TCERG1 and Intestine
 Eye Antibodies  >1 publications with TCERG1 and Eye
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