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GATAD1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS120070

MBS120070 is an antibody targeting GATAD1. Also known as Mouse monoclonal antibody Anti-Human GATAD1. Host: Mouse. More details

$575 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GATAD1, Monoclonal Antibody
Full Product Name
Mouse monoclonal antibody Anti-Human GATAD1
Product Gene Name
anti-GATAD1 antibody
Product Synonym Names
GATA zinc finger domain containing 1 (GATAD1); ODAG; FLJ22489; RG083M05.2
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
GATA9A1D3
Host
Mouse
Form/Format
Mouse monoclonal anti-human GATADI antibody in PBS (3.0 mM KCI ,1.5 mM KH2PO4 140 mM NaCI , 8.0 mM Na2HPO4(pH7.4)) containing 1% bovine serum albumin (BSA)and 0.05% sodium azide (NaN3)
Concentration
100 ug/ml (1.0 ml) (lot specific)
Preparation and Storage
Store at 2°C to 8°C for up to one year.
We recommend storing at -20°C for long term storage.
Avoid repeat freezing and thawing cycles.
OMIM
614518
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Disposal
This antibody solution contains sodium azide NaN3 as a preservative. There is a potential hazard that NaN3 reacts with copper or lead to produce an explosive compound. For safe disposal , the vial has to be washed thoroughly with water.
Immunogen
Recombinant
Applications Tested/Suitable for anti-GATAD1 antibody
Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC)
Application Notes for anti-GATAD1 antibody
WB: 0.2-2ug/mL
FC:0.5-2ug/mL
ICC: 2-100ug/mL

Quality Control of anti-GATAD1 antibody

Western blot analysis of immunized recombinant protein, using anti-GATAD1 monoclonal antibody.
Mouse Anti-GATAD1 Monoclonal Antibody – Cat# MBS120070

Quality Control #2 of anti-GATAD1 antibody

Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Mouse Anti-GATAD1 Monoclonal Antibody, Quality Control #2 – Cat# MBS120070

Western Blot (WB) of anti-GATAD1 antibody

Detection of GATAD1 by Western blot.

Samples: Whole cell lysate from human A2058 (H, 50 ug) and mouse NIH3T3 (M, 50 ug) cells. [Lot No. GATA9A1D3-1]

Predicted molecular weight: 28 kDa
Mouse Anti-GATAD1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS120070

Flow Cytometry (FC/FACS) of anti-GATAD1 antibody

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-GATAD1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. GATA9A1D3-1]
Mouse Anti-GATAD1 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS120070

Immunocytochemistry (ICC) of anti-GATAD1 antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-GATAD1 mAb. [Lot No. GATA9A1D3-1]
Mouse Anti-GATAD1 Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS120070

Other Notes

Small volumes of anti-GATAD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GATAD1. It may not necessarily be applicable to this product.

NCBI GI #
392307002
NCBI GeneID
57798
NCBI Accession #
NP_066990.3
UniProt Secondary Accession #
Q8N5Y5; Q99995; Q9H689; B2RE37; D6W5Q5
UniProt Related Accession #
Q8WUU5
GenBank Nucleotide #
NM_021167
NCBI Official Full Name
Homo sapiens GATA zinc finger domain containing 1 (GATAD1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
GATA zinc finger domain containing 1
NCBI Official Symbol
GATAD1
NCBI Official Synonym Symbols
ODAG; CMD2B; RG083M05.2
UniProt Protein Name
GATA zinc finger domain-containing protein 1
UniProt Synonym Protein Names
Ocular development-associated gene protein
Protein Family
GATA zinc finger domain-containing protein
UniProt Gene Name
GATAD1
UniProt Entry Name
GATD1_HUMAN
NCBI Protein Information
GATA zinc finger domain-containing protein 1; ocular development-associated gene protein

NCBI Summary for GATAD1

The protein encoded by this gene contains a zinc finger at the N-terminus, and is thought to bind to a histone modification site that regulates gene expression. Mutations in this gene have been associated with autosomal recessive dilated cardiomyopathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2012]
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UniProt Comments for GATAD1

GATAD1: Component of some chromatin complex recruited to chromatin sites methylated 'Lys-4' of histone H3 (H3K4me). Defects in GATAD1 are the cause of cardiomyopathy, dilated type 2B (CMD2B). CMD2B is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 7q21-q22

Cellular Component: nucleoplasm; nucleus

Molecular Function: zinc ion binding; sequence-specific DNA binding; transcription factor activity

Biological Process: regulation of transcription, DNA-dependent

Disease: Cardiomyopathy, Dilated, 2b
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Research Articles on GATAD1

1. GATAD1 expression was significantly decreased in the third-trimester placentas associated with preeclampsia than those associated with normal pregnancy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GATAD1 antibody

 Reference Product  PubMed Publications
 PEX1 antibody  >1 publications with GATAD1 and PEX1

Diseases associated with anti-GATAD1 antibody

 Disease Name  Pubmed Publications
 Heart Failure Antibodies  >2 publications with GATAD1 and Heart Failure

Organs/Tissues associated with anti-GATAD1 antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >4 publications with GATAD1 and Eye
 Heart Antibodies  >2 publications with GATAD1 and Heart
 Placenta Antibodies  >1 publications with GATAD1 and Placenta
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