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BRD3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS120113

This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is… More details

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Product Overview

Online Datasheet in PDF
Product Name
BRD3, Monoclonal Antibody
Full Product Name
Mouse monoclonal antibody Anti-Human BRD3
Product Gene Name
anti-BRD3 antibody
Product Synonym Names
Homo sapiens bromodomain containing 3 (BRD3), mRNA; ORFX; RING3L; KIAA0043
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2088C3a
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Molecular Weight
60,942 Da
OMIM
601541
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Applications Tested/Suitable for anti-BRD3 antibody
Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS)

Quality Control of anti-BRD3 antibody

Western blot analysis of immunized recombinant protein, using anti-BRD3 monoclonal antibody.
Mouse Anti-BRD3 Monoclonal Antibody – Cat# MBS120113

Quality Control #2 of anti-BRD3 antibody

Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Mouse Anti-BRD3 Monoclonal Antibody, Quality Control #2 – Cat# MBS120113

Western Blot (WB) of anti-BRD3 antibody

Detection of BRD3 by Western blot.

Samples: Whole cell lysate from human HeLa (H, 50 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. 2088C3a-1]

Predicted molecular weight: 79 kDa
Mouse Anti-BRD3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS120113

Immunoprecipitation (IP) of anti-BRD3 antibody

Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-BRD3 mAb. [Lot No. 2088C3a-1]

Predicted molecular weight: 79 kDa
Mouse Anti-BRD3 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS120113

Flow Cytometry (FC/FACS) of anti-BRD3 antibody

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-BRD3 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2088C3a-1]
Mouse Anti-BRD3 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS120113

Immunocytochemistry (ICC) of anti-BRD3 antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BRD3 mAb. [Lot No. 2088C3a-1]
Mouse Anti-BRD3 Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS120113

Other Notes

Small volumes of anti-BRD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BRD3 purchase

MBS120113 is an antibody targeting BRD3. Also known as Mouse monoclonal antibody Anti-Human BRD3. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for BRD3. It may not necessarily be applicable to this product.

NCBI GI #
207028804
NCBI GeneID
8019
NCBI Accession #
NP_031397.1
UniProt Secondary Accession #
Q4G5Y3; Q5T1R7; Q8N5M3; Q92645; B1APD9
UniProt Related Accession #
Q15059
GenBank Nucleotide #
NM_007371
NCBI Official Full Name
Homo sapiens bromodomain containing 3 (BRD3), mRNA
NCBI Official Synonym Full Names
bromodomain containing 3
NCBI Official Symbol
BRD3
NCBI Official Synonym Symbols
ORFX; RING3L
UniProt Protein Name
Bromodomain-containing protein 3
UniProt Synonym Protein Names
RING3-like protein
Protein Family
Bromodomain-containing protein
UniProt Gene Name
BRD3
UniProt Entry Name
BRD3_HUMAN
NCBI Protein Information
bromodomain-containing protein 3; RING3-like protein; bromodomain-containing 3

UniProt Comments for BRD3

BRD3: Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets. Regulates transcription of the CCND1 gene. A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, atypical; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; BRD family

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: nucleus

Molecular Function: protein binding; chromatin binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; chromatin modification
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Research Articles on BRD3

1. Here, we identify NCT, a complex comprising the Nrc1 BET-family tandem bromodomain protein (SPAC631.02), casein kinase II (CKII), and several TAFs, as a regulator of condensin function.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BRD3 antibody

 Reference Product  PubMed Publications
 BRD4 antibody  >48 publications with BRD3 and BRD4
 BRD7 antibody  >3 publications with BRD3 and BRD7

Diseases associated with anti-BRD3 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >6 publications with BRD3 and Inflammation
 Infertility, Male Antibodies  >2 publications with BRD3 and Infertility, Male
 Disease Models, Animal Antibodies  >2 publications with BRD3 and Disease Models, Animal
 Arthritis, Experimental Antibodies  >1 publications with BRD3 and Arthritis, Experimental
 Brain Diseases Antibodies  >1 publications with BRD3 and Brain Diseases
 Congenital Abnormalities Antibodies  >1 publications with BRD3 and Congenital Abnormalities
 Liver Diseases Antibodies  >1 publications with BRD3 and Liver Diseases
 Hypertrophy Antibodies  >1 publications with BRD3 and Hypertrophy
 Immune System Diseases Antibodies  >1 publications with BRD3 and Immune System Diseases
 Urinary Bladder Neoplasms Antibodies  >1 publications with BRD3 and Urinary Bladder Neoplasms

Organs/Tissues associated with anti-BRD3 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >13 publications with BRD3 and Testis
 Blood Antibodies  >4 publications with BRD3 and Blood
 Lung Antibodies  >4 publications with BRD3 and Lung
 Muscle Antibodies  >4 publications with BRD3 and Muscle
 Brain Antibodies  >4 publications with BRD3 and Brain
 Bone Antibodies  >3 publications with BRD3 and Bone
 Embryonic Tissue Antibodies  >2 publications with BRD3 and Embryonic Tissue
 Kidney Antibodies  >2 publications with BRD3 and Kidney
 Pancreas Antibodies  >2 publications with BRD3 and Pancreas
 Ovary Antibodies  >2 publications with BRD3 and Ovary
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