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GTF2A1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS120230

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. Interacts with TBP (the TATA-binding protein). [UniProtKB Function] More details

$575 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GTF2A1, Monoclonal Antibody
Full Product Name
Mouse monoclonal antibody Anti-Human GTF2A1
Product Gene Name
anti-GTF2A1 antibody
Product Synonym Names
Homo sapiens general transcription factor IIA, 1, 19/37kDa (GTF2A1), transcript variant 1; TF2A1; TFIIA; MGC129969; MGC129970
Clonality
Monoclonal
Isotype
IgG1
Clone Number
708C4a
Host
Mouse
Species Reactivity
Human
Form/Format
Mouse monoclonal anti-human GTF2A1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2PO4, 140 mM NaCl, 8.0 mM Na2HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3
Concentration
100 ug/ml (1.0 ml) (lot specific)
Preparation and Storage
Store at 2-8°C for up to one year.
We recommend storing at -20°C for long term storage.
Avoid repeat freezing and thawing cycles.
Molecular Weight
37,152 Da
OMIM
600520
Immunogen
Recombinant
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Disposal
This antibody solution contains sodium azide (NaN3) as a preservative. There is a potential hazard that NaN3 reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water.
Safety warning and precautions
Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off.
Applications Tested/Suitable for anti-GTF2A1 antibody
Dot Blot (DB)

Quality Control of anti-GTF2A1 antibody

Western blot analysis of immunized recombinant protein, using anti-GTF2A1 monoclonal antibody.
Mouse Anti-GTF2A1 Monoclonal Antibody – Cat# MBS120230

Quality Control #2 of anti-GTF2A1 antibody

Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Mouse Anti-GTF2A1 Monoclonal Antibody, Quality Control #2 – Cat# MBS120230

Other Notes

Small volumes of anti-GTF2A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GTF2A1 purchase

MBS120230 is an antibody targeting GTF2A1. Also known as Mouse monoclonal antibody Anti-Human GTF2A1. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GTF2A1. It may not necessarily be applicable to this product.

NCBI GI #
387763630
NCBI GeneID
2957
NCBI Accession #
NP_056943.1
UniProt Secondary Accession #
Q3KNQ9
UniProt Related Accession #
P52655
GenBank Nucleotide #
NM_015859
NCBI Official Full Name
Homo sapiens general transcription factor IIA, 1, 19/37kDa (GTF2A1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
general transcription factor IIA, 1, 19/37kDa
NCBI Official Symbol
GTF2A1
NCBI Official Synonym Symbols
TF2A1; TFIIA; TFIIAL; TFIIA-42
UniProt Protein Name
Transcription initiation factor IIA subunit 1
UniProt Synonym Protein Names
General transcription factor IIA subunit 1; TFIIAL; Transcription initiation factor TFIIA 42 kDa subunit; TFIIA-42
Protein Family
Transcription initiation factor
UniProt Gene Name
GTF2A1
UniProt Entry Name
TF2AA_HUMAN
NCBI Protein Information
transcription initiation factor IIA subunit 1; TFIIA alpha, p55, isoform 1; general transcription factor IIA subunit 1; glucose regulated protein, 58kD pseudogene; transcription initiation factor TFIIA 42 kDa subunit

NCBI Summary for GTF2A1

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010]
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UniProt Comments for GTF2A1

GTF2A1: a general transcription factor that plays an important role in transcriptional activation. A component of the transcription machinery of RNA polymerase II. Phosphorylated by Taf(II) 250 on serines that regulate TBP binding. Interacts with TBP (the TATA-binding protein). Two isoforms, 42 kDa and 37 kDa, are produced by alternative initiation.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 14q31.1

Cellular Component: nucleoplasm; cytoplasm; transcription factor TFIIA complex

Molecular Function: protein binding; DNA binding; TATA-binding protein binding; protein heterodimerization activity; transcription coactivator activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; regulation of transcription, DNA-dependent; RNA elongation from RNA polymerase II promoter; gene expression
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Research Articles on GTF2A1

1. GTF2A1 alone, or GTF2A1 plus HAAO are excellent candidate biomarkers for detecting Ovarian cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GTF2A1 antibody

 Reference Product  PubMed Publications
 GTF2A2 antibody  >1 publications with GTF2A1 and GTF2A2
 TBP antibody  >1 publications with GTF2A1 and TBP

Pathways associated with anti-GTF2A1 antibody

 Products by Pathway  Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 HIV Life Cycle Pathway antibodies  HIV Life Cycle Pathway Diagram
 HIV Transcription Initiation Pathway antibodies  HIV Transcription Initiation Pathway Diagram
 Late Phase Of HIV Life Cycle Pathway antibodies  Late Phase Of HIV Life Cycle Pathway Diagram
 RNA Polymerase II HIV Promoter Escape Pathway antibodies  RNA Polymerase II HIV Promoter Escape Pathway Diagram
 RNA Polymerase II Pre-transcription Events Pathway antibodies  RNA Polymerase II Pre-transcription Events Pathway Diagram

Diseases associated with anti-GTF2A1 antibody

 Disease Name  Pubmed Publications
 Ovarian Neoplasms Antibodies  >1 publications with GTF2A1 and Ovarian Neoplasms

Organs/Tissues associated with anti-GTF2A1 antibody

 Organ/Tissue Name  Pubmed Publications
 Stomach Antibodies  >1 publications with GTF2A1 and Stomach
 Testis Antibodies  >1 publications with GTF2A1 and Testis
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