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TPX2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS120232

MBS120232 is an antibody targeting TPX2. Also known as Mouse monoclonal antibody Anti-Human TPX2. Reactive in Human. Host: Mouse. More details

$575 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TPX2, Monoclonal Antibody
Full Product Name
Mouse monoclonal antibody Anti-Human TPX2
Product Gene Name
Product Synonym Names
Homo sapiens TPX2, microtubule-associated, homolog (Xenopus laevis) (TPX2); DIL2; p100; DIL-2; HCTP4; FLS353;HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3164C6a
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Molecular Weight
89,393 Da
OMIM
605917
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Applications Tested/Suitable for anti-TPX2 antibody
Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS)

Quality Control of anti-TPX2 antibody

Western blot analysis of immunized recombinant protein, using anti-TPX2 monoclonal antibody.
Mouse Anti-TPX2 Monoclonal Antibody – Cat# MBS120232

Quality Control #2 of anti-TPX2 antibody

Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Mouse Anti-TPX2 Monoclonal Antibody, Quality Control #2 – Cat# MBS120232

Western Blot (WB) of anti-TPX2 antibody

Detection of human TPX2 by Western blot.

Samples: Whole cell lysate (25 ug) from HeLa cells. [Lot No. 3164C6a-1]

Predicted molecular weight: 85 kDa
Mouse Anti-TPX2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS120232

Immunoprecipitation (IP) of anti-TPX2 antibody

Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-TPX2 mAb. [Lot No. 3164C6a-1]

Predicted molecular weight: 85 kDa
Mouse Anti-TPX2 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS120232

Flow Cytometry (FC/FACS) of anti-TPX2 antibody

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TPX2 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 3164C6a-1]
Mouse Anti-TPX2 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS120232

Immunocytochemistry (ICC) of anti-TPX2 antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TPX2 mAb. Nuclear was stained with Hoechst (blue fluorescence). [Lot No. 3164C6a-1]
Mouse Anti-TPX2 Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS120232

Other Notes

Small volumes of anti-TPX2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TPX2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens TPX2, microtubule-associated (TPX2), mRNA
NCBI Official Synonym Full Names
TPX2, microtubule-associated
NCBI Official Symbol
TPX2
NCBI Official Synonym Symbols
DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
UniProt Protein Name
Targeting protein for Xklp2
UniProt Synonym Protein Names
Differentially expressed in cancerous and non-cancerous lung cells 2; DIL-2; Hepatocellular carcinoma-associated antigen 519; Hepatocellular carcinoma-associated antigen 90; Protein fls353; Restricted expression proliferation-associated protein 100; p100
Protein Family
UniProt Gene Name
TPX2
UniProt Entry Name
TPX2_HUMAN
NCBI Protein Information
targeting protein for Xklp2; TPX2, microtubule-associated protein homolog; TPX2, microtubule-associated, homolog; differentially expressed in cancerous and non-cancerous lung cells 2; differentially expressed in lung cells; hepatocellular carcinoma-associated antigen 519; hepatocellular carcinoma-associated antigen 90; preferentially expressed in colorectal cancer; protein fls353; restricted expression proliferation associated protein 100; restricted expression proliferation-associated protein 100

UniProt Comments for TPX2

TPX2: a microtubule-associated protein required for meiotic and mitotic spindle assembly. Targets a plus end-directed motor (XKLP2) to the minus ends of microtubules in the mitotic spindle. Required for microtubule nucleation around chromosomes in response to the local generation of RanGTP. Binds to and activates Aurora A kinase and targets it to the mitotic spindle.

Protein type: Microtubule-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: axon hillock; nucleoplasm; microtubule cytoskeleton; spindle pole; microtubule; cytoplasm; spindle; nucleus

Molecular Function: protein binding; GTP binding; protein kinase binding; ATP binding

Biological Process: cell proliferation; mitosis; apoptosis; cell division; activation of protein kinase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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