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POLR2G Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS120241

The homeodomain-containing transcription factor NKX3A is a putative prostate tumor suppressor that is expressed in a largely prostate-specific and androgen-regulated manner. Loss of NKX3A protein expression is a common finding in human prostate carcinomas and… More details

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Product Overview

Online Datasheet in PDF
Product Name
POLR2G, Monoclonal Antibody
Full Product Name
Mouse monoclonal antibody Anti-Human POLR2G
Product Gene Name
anti-POLR2G antibody
Product Synonym Names
Homo sapiens polymerase (RNA) II (DNA directed) polypeptide G (POLR2G); RPB7; hRPB19; hsRPB7; MGC138367; MGC138369
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2781C2a
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Molecular Weight
19,294 Da
OMIM
602013
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Applications Tested/Suitable for anti-POLR2G antibody
Dot Blot (DB), Western Blot (WB), Flow Cytometry (FC/FACS)

Quality Control of anti-POLR2G antibody

Western blot analysis of immunized recombinant protein, using anti-POLR2G monoclonal antibody.
Mouse Anti-POLR2G Monoclonal Antibody – Cat# MBS120241

Quality Control #2 of anti-POLR2G antibody

Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Mouse Anti-POLR2G Monoclonal Antibody, Quality Control #2 – Cat# MBS120241

Western Blot (WB) of anti-POLR2G antibody

Detection of POLR2G by Western blot.

Samples: Whole cell lysate from human HT-1080 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 2781C2a-1]

Predicted molecular weight: 19 kDa
Mouse Anti-POLR2G Monoclonal Antibody, Western Blot (WB) – Cat# MBS120241

Flow Cytometry (FC/FACS) of anti-POLR2G antibody

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-POLR2G mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2781C2a-1]
Mouse Anti-POLR2G Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS120241

Other Notes

Small volumes of anti-POLR2G antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLR2G purchase

MBS120241 is an antibody targeting POLR2G. Also known as Mouse monoclonal antibody Anti-Human POLR2G. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for POLR2G. It may not necessarily be applicable to this product.

NCBI GI #
219879812
NCBI GeneID
5436
NCBI Accession #
NP_002687.1
UniProt Secondary Accession #
P52433; Q2M1Z4; B2R5C0
UniProt Related Accession #
P62487
GenBank Nucleotide #
NM_002696
NCBI Official Full Name
Homo sapiens polymerase (RNA) II (DNA directed) polypeptide G (POLR2G), mRNA
NCBI Official Synonym Full Names
polymerase (RNA) II (DNA directed) polypeptide G
NCBI Official Symbol
POLR2G
NCBI Official Synonym Symbols
RPB7; RPB19; hRPB19; hsRPB7
UniProt Gene Name
POLR2G
UniProt Entry Name
RPB7_HUMAN
NCBI Protein Information
DNA-directed RNA polymerase II subunit RPB7; DNA directed RNA polymerase II 19 kda polypeptide; DNA-directed RNA polymerase II 19 kDa polypeptide; DNA-directed RNA polymerase II subunit G; RNA polymerase II 19 kDa subunit; RNA polymerase II seventh subunit; RNA polymerase II subunit B7

NCBI Summary for POLR2G

This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The protein functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation. [provided by RefSeq, Jan 2009]
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UniProt Comments for POLR2G

POLR2G: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. Binds RNA. Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.

Protein type: Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: nucleoplasm; nucleolus; DNA-directed RNA polymerase II, core complex; nucleus

Molecular Function: single-stranded RNA binding; DNA-directed RNA polymerase activity; translation initiation factor binding; single-stranded DNA binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; somatic stem cell maintenance; apoptosis; mRNA catabolic process, exonucleolytic; RNA splicing; DNA repair; positive regulation of translational initiation; nuclear mRNA splicing, via spliceosome; mRNA capping; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; gene expression
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Research Articles on POLR2G

1. The function of Rpb7 interaction with rpb4 in human cells indicate that Rpb7 have gene-specific effects but are also more generally required for human cell survival.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-POLR2G antibody

 Reference Product  PubMed Publications
 POLR2D antibody  >2 publications with POLR2G and POLR2D

Pathways associated with anti-POLR2G antibody

 Products by Pathway  Pathway Diagram
 Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway antibodies  Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Dual Incision Reaction In TC-NER Pathway antibodies  Dual Incision Reaction In TC-NER Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Eukaryotic Transcription Initiation Pathway antibodies  Eukaryotic Transcription Initiation Pathway Diagram
 Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway antibodies  Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway Diagram
 Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway antibodies  Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway Diagram

Diseases associated with anti-POLR2G antibody

 Disease Name  Pubmed Publications
 Death Antibodies  >2 publications with POLR2G and Death
 Cardiovascular Diseases Antibodies  >1 publications with POLR2G and Cardiovascular Diseases
 Kidney Diseases Antibodies  >1 publications with POLR2G and Kidney Diseases

Organs/Tissues associated with anti-POLR2G antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >2 publications with POLR2G and Kidney
 Blood Antibodies  >1 publications with POLR2G and Blood
 Vascular Antibodies  >1 publications with POLR2G and Vascular
 Intestine Antibodies  >1 publications with POLR2G and Intestine
 Eye Antibodies  >1 publications with POLR2G and Eye
 Liver Antibodies  >1 publications with POLR2G and Liver
 Stomach Antibodies  >1 publications with POLR2G and Stomach
 Lung Antibodies  >1 publications with POLR2G and Lung
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