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TAF9L Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS120263

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly,… More details

$575 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
TAF9L, Monoclonal Antibody
Full Product Name
Mouse monoclonal antibody Anti-Human TAF9L
Product Gene Name
anti-TAF9L antibody
Product Synonym Names
Homo sapiens TAF9B RNA polymerase II, TATA box binding protein(TBP)-associated factor, 31kDa (TAF9B); TAF9B;DN7; DN-7;TAFII31L; TFIID-31
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3365C4a
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Molecular Weight
27,622 Da
OMIM
300754
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Applications Tested/Suitable for anti-TAF9L antibody
Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB)

Quality Control of anti-TAF9L antibody

Western blot analysis of immunized recombinant protein, using anti-TAF9L monoclonal antibody.
Mouse Anti-TAF9L Monoclonal Antibody – Cat# MBS120263

Quality Control #2 of anti-TAF9L antibody

Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Mouse Anti-TAF9L Monoclonal Antibody, Quality Control #2 – Cat# MBS120263

Western Blot (WB) of anti-TAF9L antibody

Detection of TAF9L by Western blot.

Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 3365C4a-1]

Predicted molecular weight: 27 kDa
Mouse Anti-TAF9L Monoclonal Antibody, Western Blot (WB) – Cat# MBS120263

Immunocytochemistry (ICC) of anti-TAF9L antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TAF9L mAb. [Lot No. 3365C4a-1]
Mouse Anti-TAF9L Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS120263

Other Notes

Small volumes of anti-TAF9L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TAF9L purchase

MBS120263 is an antibody targeting TAF9L. Also known as Mouse monoclonal antibody Anti-Human TAF9L. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TAF9L. It may not necessarily be applicable to this product.

NCBI GI #
85062626
NCBI GeneID
51616
NCBI Accession #
NP_057059.2
UniProt Secondary Accession #
Q9Y2S3; B2RUZ9
UniProt Related Accession #
Q9HBM6
GenBank Nucleotide #
NM_015975
NCBI Official Full Name
Homo sapiens TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa (TAF9B), mRNA
NCBI Official Synonym Full Names
TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa
NCBI Official Symbol
TAF9B
NCBI Official Synonym Symbols
DN7; DN-7; TAF9L; TAFII31L; TFIID-31
UniProt Protein Name
Transcription initiation factor TFIID subunit 9B
UniProt Synonym Protein Names
Neuronal cell death-related protein 7; DN-7; Transcription initiation factor TFIID subunit 9-like; Transcription-associated factor TAFII31L
UniProt Gene Name
TAF9B
UniProt Entry Name
TAF9B_HUMAN
NCBI Protein Information
transcription initiation factor TFIID subunit 9B; TAF9-like RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa; TBP-associated factor 9L; neuronal cell death-related protein 7; transcription associated factor TAFII31L; transcription initiation factor IID, 31kD subunit; transcription-associated factor TAFII31L

UniProt Comments for TAF9L

TAF9B: Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription. Belongs to the TAF9 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: Xq13.1-q21.1

Cellular Component: nucleoplasm; transcription factor TFIID complex

Molecular Function: protein binding; protein heterodimerization activity; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; protein stabilization; viral reproduction; RNA elongation from RNA polymerase II promoter; gene expression; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell growth; negative regulation of apoptosis
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Research Articles on TAF9L

1. TAF9b (TAF9L) was identified as a subunit of TFIID.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TAF9L antibody

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