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KNTC2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS120269

HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar… More details

$575 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
KNTC2, Monoclonal Antibody
Full Product Name
Mouse monoclonal antibody Anti-Human KNTC2
Product Gene Name
anti-KNTC2 antibody
Product Synonym Names
Homo sapiens NDC80 homolog, kinetochore complex component (S.cerevisiae) (NDC80); NDC80; HEC; HEC1; TID3; hsNDC80
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2497C3a
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Molecular Weight
73,913 Da
OMIM
607272
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Applications Tested/Suitable for anti-KNTC2 antibody
Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB)

Quality Control of anti-KNTC2 antibody

Western blot analysis of immunized recombinant protein, using anti-KNTC2 monoclonal antibody.
Mouse Anti-KNTC2 Monoclonal Antibody – Cat# MBS120269

Quality Control #2 of anti-KNTC2 antibody

Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Mouse Anti-KNTC2 Monoclonal Antibody, Quality Control #2 – Cat# MBS120269

Western Blot (WB) of anti-KNTC2 antibody

Detection of human KNTC2 by Western blot.

Samples: Whole cell lysate (25 ug) from HeLa cells. [Lot No. 2497C3a-1]

Predicted molecular weight: 73 kDa
Mouse Anti-KNTC2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS120269

Immunocytochemistry (ICC) of anti-KNTC2 antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-KNTC2 mAb. Nuclear was stained with Hoechst (blue fluorescence). [Lot No. 2497C3a-1]
Mouse Anti-KNTC2 Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS120269

Other Notes

Small volumes of anti-KNTC2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KNTC2 purchase

MBS120269 is an antibody targeting KNTC2. Also known as Mouse monoclonal antibody Anti-Human KNTC2. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KNTC2. It may not necessarily be applicable to this product.

NCBI GI #
215820615
NCBI GeneID
10403
NCBI Accession #
NP_006092.1
UniProt Secondary Accession #
Q6PJX2
UniProt Related Accession #
O14777
GenBank Nucleotide #
NM_006101
NCBI Official Full Name
Homo sapiens NDC80 kinetochore complex component (NDC80), mRNA
NCBI Official Synonym Full Names
NDC80 kinetochore complex component
NCBI Official Symbol
NDC80
NCBI Official Synonym Symbols
HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
UniProt Protein Name
Kinetochore protein NDC80 homolog
UniProt Synonym Protein Names
Highly expressed in cancer protein; Kinetochore protein Hec1; HsHec1; Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC
UniProt Gene Name
NDC80
UniProt Entry Name
NDC80_HUMAN
NCBI Protein Information
kinetochore protein NDC80 homolog; NDC80 homolog, kinetochore complex component; NDC80 kinetochore complex component homolog; highly expressed in cancer protein; highly expressed in cancer, rich in leucine heptad repeats; kinetochore associated 2; kinetochore protein Hec1; kinetochore-associated protein 2; retinoblastoma-associated protein HEC

NCBI Summary for KNTC2

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]
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UniProt Comments for KNTC2

HEC1: plays an essential role in chromosome segregation by interacting through its coiled-coil domains with several proteins that modulate the G2/M phase. Phosphorylation by Nek2 is essential for faithful chromosome segregation. Required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: kinetochore; membrane; cytosol; nucleus; chromosome, pericentric region

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: mitosis; mitotic spindle organization and biogenesis; attachment of spindle microtubules to kinetochore during mitosis; mitotic sister chromatid segregation; establishment of mitotic spindle orientation; cell division; mitotic cell cycle; attachment of spindle microtubules to kinetochore; chromosome segregation
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Research Articles on KNTC2

1. Mechanisms of mitosis-specific assembly of the checkpoint platform Knl1/MIS12/NDC80 at human kinetochores.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KNTC2 antibody

 Reference Product  PubMed Publications
 NUF2 antibody  >50 publications with KNTC2 and NUF2
 MIS12 antibody  >43 publications with KNTC2 and MIS12
 SPC24 antibody  >32 publications with KNTC2 and SPC24
 SPC25 antibody  >26 publications with KNTC2 and SPC25
 AURKB antibody  >24 publications with KNTC2 and AURKB
 BUB1 antibody  >16 publications with KNTC2 and BUB1
 ZWINT antibody  >9 publications with KNTC2 and ZWINT
 SKA1 antibody  >5 publications with KNTC2 and SKA1

Pathways associated with anti-KNTC2 antibody

 Products by Pathway  Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic Anaphase Pathway antibodies  Mitotic Anaphase Pathway Diagram
 Mitotic Metaphase And Anaphase Pathway antibodies  Mitotic Metaphase And Anaphase Pathway Diagram
 Mitotic Prometaphase Pathway antibodies  Mitotic Prometaphase Pathway Diagram
 PLK1 Signaling Events Pathway antibodies  PLK1 Signaling Events Pathway Diagram
 Resolution Of Sister Chromatid Cohesion Pathway antibodies  Resolution Of Sister Chromatid Cohesion Pathway Diagram
 Separation Of Sister Chromatids Pathway antibodies  Separation Of Sister Chromatids Pathway Diagram

Diseases associated with anti-KNTC2 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >8 publications with KNTC2 and Carcinoma
 Breast Neoplasms Antibodies  >6 publications with KNTC2 and Breast Neoplasms
 Ovarian Neoplasms Antibodies  >5 publications with KNTC2 and Ovarian Neoplasms
 Lung Neoplasms Antibodies  >5 publications with KNTC2 and Lung Neoplasms
 Cell Transformation, Neoplastic Antibodies  >4 publications with KNTC2 and Cell Transformation, Neoplastic
 Adenocarcinoma Antibodies  >4 publications with KNTC2 and Adenocarcinoma
 Lung Diseases Antibodies  >3 publications with KNTC2 and Lung Diseases
 Neoplasms, Experimental Antibodies  >3 publications with KNTC2 and Neoplasms, Experimental
 Liver Neoplasms Antibodies  >2 publications with KNTC2 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >2 publications with KNTC2 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-KNTC2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with KNTC2 and Blood
 Lung Antibodies  >5 publications with KNTC2 and Lung
 Brain Antibodies  >4 publications with KNTC2 and Brain
 Heart Antibodies  >3 publications with KNTC2 and Heart
 Stomach Antibodies  >3 publications with KNTC2 and Stomach
 Liver Antibodies  >2 publications with KNTC2 and Liver
 Prostate Antibodies  >1 publications with KNTC2 and Prostate
 Cervix Antibodies  >1 publications with KNTC2 and Cervix
 Ovary Antibodies  >1 publications with KNTC2 and Ovary
 Skin Antibodies  >1 publications with KNTC2 and Skin
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