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ATF3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS120339

Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for… More details

$575 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ATF3, Monoclonal Antibody
Full Product Name
Mouse monoclonal antibody Anti-Human ATF3
Product Gene Name
anti-ATF3 antibody
Product Synonym Names
Homo sapiens activating transcription factor 3
Clonality
Monoclonal
Isotype
IgG1
Clone Number
44C3a
Host
Mouse
Species Reactivity
Human
Form/Format
Mouse Monoclonal anti human ATF3 antibody in PBS (3.0 mM KCl, 1.5mM KH2PO4, 140 mM NaCl, 8.0 mM Na2HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3
Preparation and Storage
Store at 2-8°C for up to one year.
We recommend storing at -20°C for long term storage.
Avoid repeat freezing and thawing cycles.
OMIM
603148
Preparation
This antibody was purified using protein A column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Disposal
This antibody solution contains sodium azide (NaN3)as a preservative. There is a potential hazard that NaN3 reacts with copper or lead to produc an explosive compound. For safe disposal, the vial has to be washed thorughly with water.
Safety warning and precautions
Caution must be taken to avoid contact with skin or eyes. In such case, rinse thoroughly at once with water. Do not ingest, inhale or swallow. Seek medical attention inmediately. Wear appropiate protective clothing such as laboratory overalls, safety glasess and gloves. It is strongly advised that this product should be handled by people who have been well traied in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off.
Applications Tested/Suitable for anti-ATF3 antibody
Immunocytochemistry (ICC)
Application Notes for anti-ATF3 antibody
ICC: 2-100 ug/ml

Quality Control of anti-ATF3 antibody

Western blot analysis of immunized recombinant protein, using anti-ATF3 monoclonal antibody.
Mouse Anti-ATF3 Monoclonal Antibody – Cat# MBS120339

Quality Control #2 of anti-ATF3 antibody

Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Mouse Anti-ATF3 Monoclonal Antibody, Quality Control #2 – Cat# MBS120339

Immunocytochemistry (ICC) of anti-ATF3 antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-ATF3 mAb.
Mouse Anti-ATF3 Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS120339

Other Notes

Small volumes of anti-ATF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATF3 purchase

MBS120339 is an antibody targeting ATF3. Also known as Mouse monoclonal antibody Anti-Human ATF3. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ATF3. It may not necessarily be applicable to this product.

NCBI GI #
95102480
NCBI GeneID
467
NCBI Accession #
NP_004015.4
UniProt Secondary Accession #
Q5VTZ2; Q6ICQ9; Q7Z566; Q8WYM6
UniProt Related Accession #
P18847
GenBank Nucleotide #
NM_004024
NCBI Official Synonym Full Names
activating transcription factor 3
NCBI Official Symbol
ATF3
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-3
UniProt Synonym Protein Names
Activating transcription factor 3
Protein Family
Activating transcription factor
UniProt Gene Name
ATF3
UniProt Entry Name
ATF3_HUMAN
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-3

NCBI Summary for ATF3

This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]
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UniProt Comments for ATF3

ATF-3: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Belongs to the bZIP family. ATF subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; transcription factor activity; transcription corepressor activity

Biological Process: cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; transcription, DNA-dependent; unfolded protein response; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; gluconeogenesis
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Research Articles on ATF3

1. Human CHAC1 Protein Degrades Glutathione, and mRNA Induction Is Regulated by the Transcription Factors ATF4 and ATF3 and a Bipartite ATF/CRE Regulatory Element.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ATF3 antibody

 Reference Product  PubMed Publications
 JUN antibody  >205 publications with ATF3 and JUN
 ATF4 antibody  >71 publications with ATF3 and ATF4
 DDIT3 antibody  >62 publications with ATF3 and DDIT3
 EGR1 antibody  >42 publications with ATF3 and EGR1
 TP53 antibody  >16 publications with ATF3 and TP53
 KLF6 antibody  >5 publications with ATF3 and KLF6
 SMAD4 antibody  >4 publications with ATF3 and SMAD4
 PDX1 antibody  >2 publications with ATF3 and PDX1
 IFNG antibody  >1 publications with ATF3 and IFNG
 SMARCA4 antibody  >1 publications with ATF3 and SMARCA4

Pathways associated with anti-ATF3 antibody

 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 ATF4 Activates Genes Pathway antibodies  ATF4 Activates Genes Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Hypertrophy Model Pathway antibodies  Hypertrophy Model Pathway Diagram
 Id Signaling Pathway antibodies  Id Signaling Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 PERK Regulates Gene Expression Pathway antibodies  PERK Regulates Gene Expression Pathway Diagram

Diseases associated with anti-ATF3 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >153 publications with ATF3 and Nervous System Diseases
 Disease Models, Animal Antibodies  >123 publications with ATF3 and Disease Models, Animal
 Pain Antibodies  >110 publications with ATF3 and Pain
 Inflammation Antibodies  >105 publications with ATF3 and Inflammation
 Peripheral Nervous System Diseases Antibodies  >74 publications with ATF3 and Peripheral Nervous System Diseases
 Necrosis Antibodies  >53 publications with ATF3 and Necrosis
 Cardiovascular Diseases Antibodies  >52 publications with ATF3 and Cardiovascular Diseases
 Lung Diseases Antibodies  >28 publications with ATF3 and Lung Diseases
 Liver Diseases Antibodies  >27 publications with ATF3 and Liver Diseases
 Nerve Degeneration Antibodies  >26 publications with ATF3 and Nerve Degeneration

Organs/Tissues associated with anti-ATF3 antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >170 publications with ATF3 and Nerve
 Ganglia Antibodies  >124 publications with ATF3 and Ganglia
 Brain Antibodies  >95 publications with ATF3 and Brain
 Blood Antibodies  >91 publications with ATF3 and Blood
 Liver Antibodies  >67 publications with ATF3 and Liver
 Muscle Antibodies  >60 publications with ATF3 and Muscle
 Bone Antibodies  >50 publications with ATF3 and Bone
 Lung Antibodies  >46 publications with ATF3 and Lung
 Vascular Antibodies  >44 publications with ATF3 and Vascular
 Skin Antibodies  >44 publications with ATF3 and Skin
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