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Short-Chain-Fatty-Acid--Coa Ligase (fadK) Escherichia Coli Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1210689

MBS1210689 is a recombinant protein targeting fadK. Also known as Recombinant Escherichia coli Short-chain-fatty-acid--CoA ligase (fadK). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$1340 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Short-chain-fatty-acid--CoA ligase (fadK), Recombinant Protein
Full Product Name
Recombinant Escherichia coli Short-chain-fatty-acid--CoA ligase (fadK)
Product Gene Name
Sequence
MKVTLTFNEQ RRAAYRQQGL WGDASLADYW QQTARAMPDK IAVVDNHGAS YTYSALDHAA SCLANWMLAK GIESGDRIAF QLPGWCEFTV IYLACLKIGA VSVPLLPSWR EAELVWVLNK CQAKMFFAPT LFKQTRPVDL ILPLQNQLPQ LQQIVGVDKL APATSSLSLS QIIADNTSLT TAITTHGDEL AAVLFTSGTE GLPKGVMLTH NNILASERAY CARLNLTWQD VFMMPAPLGH ATGFLHGVTA PFLIGARSVL LDIFTPDACL ALLEQQRCTC MLGATPFVYD LLNVLEKQPA DLSALRFFLC GGTTIPKKVA RECQQRGIKL LSVYGSTESS PHAVVNLDDP LSRFMHTDGY AAAGVEIKVV DDARKTLPPG CEGEEASRGP NVFMGYFDEP ELTARALDEE GWYYSGDLCR MDEAGYIKIT GRKKDIIVRG GENISSREVE DILLQHPKIH DACVVAMSDE RLGERSCAYV VLKAPHHSLS LEEVVAFFSR KRVAKYKYPE HIVVIEKLPR TTSGKIQKFL LRKDIMRRLT QDVCEEIE
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
62,759 Da
Sequence Positions
1-548, full length protein
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for P38135
Species
Escherichia coli (strain K12)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of fadK recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for fadK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
short chain acyl-CoA synthetase, anaerobic
NCBI Official Symbol
fadK
NCBI Official Synonym Symbols
ECK1699; JW5910; ydiD
UniProt Protein Name
Medium-chain fatty-acid--CoA ligase
UniProt Synonym Protein Names
Acyl-CoA synthetase
UniProt Gene Name
fadK
UniProt Synonym Gene Names
ACS
NCBI Protein Information
short chain acyl-CoA synthetase, anaerobic

NCBI Summary for fadK

AFMB Structural Genomics target Number 54 (http://afmb.cnrs-mrs.fr/article171.html). [More information is available at EcoGene: EG12357]. FadK is an acyl-CoA synthetase that is primarily active on short chain fatty acids and acts in anaerobic -oxidation of fatty acids . [More information is available at EcoCyc: EG12357].
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UniProt Comments for fadK

Catalyzes the esterification, concomitant with transport, of exogenous fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. Is maximally active on C6:0, C8:0 and C12:0 fatty acids, while has a low activity on C14-C18 chain length fatty acids (PubMed:15213221, PubMed:19477415). Is involved in the anaerobic beta-oxidative degradation of fatty acids, which allows anaerobic growth of E.coli on fatty acids as a sole carbon and energy source in the presence of nitrate or fumarate as a terminal electron acceptor (PubMed:12535077). Can functionally replace FadD under anaerobic conditions (PubMed:12535077).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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