Human EEF2 Recombinant Protein – Cat# MBS1213669

Elongation Factor 2 (EEF2) Human Recombinant Protein

Name: EEF2 Recombinant Protein; Elongation Factor 2; Human; MyBioSource
Brand: MyBioSource
SKU: MBS1213669
Price: 315 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1213669

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and… More details

$315 / 0.02 mg (E-Coli) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Elongation factor 2 (EEF2), Recombinant Protein

Full Product Name

Recombinant Human Elongation factor 2

Product Gene Name

EEF2 recombinant protein

Product Synonym Gene Name

EF2

Sequence

VNFTVDQIRA IMDKKANIRN MSVIAHVDHG KSTLTDSLVC KAGIIASARA GETRFTDTRK DEQERCITIK STAISLFYEL SENDLNFIKQ SKDGAGFLIN LIDSPGHVDF SSEVTAALRV TDGALVVVDC VSGVCVQTET VLRQAIAERI KPVLMMNKMD RALLELQLEP EELYQTFQRI VENVNVIIST YGEGESGPMG NIMIDPVLGT VGFGSGLHGW AFTLKQFAEM YVAKFAAKGE GQLGPAERAK KVEDMMKKLW GDRYFDPANG KFSKSATSPE GKKLPRTFCQ LILDPIFKVF DAIMNFKKEE TAKLIEKLDI KLDSEDKDKE GKPLLKAVMR RWLPAGDALL QMITIHLPSP VTAQKYRCEL LYEGPPDDEA AMGIKSCDPK GPLMMYISKM VPTSDKGRFY AFGRVFSGLV STGLKVRIMG PNYTPGKKED LYLKPIQRTI LMMGRYVEPI EDVPCGNIVG LVGVDQFLVK TGTITTFEHA HNMRVMKFSV SPVVRVAVEA KNPADLPKLV EGLKRLAKSD PMVQCIIEES GEHIIAGAGE LHLEICLKDL EEDHACIPIK KSDPVVSYRE TVSEESNVLC LSKSPNKHNR LYMKARPFPD GLAEDIDKGE VSARQELKQR ARYLAEKYEW DVAEARKIWC FGPDGTGPNI LTDITKGVQY LNEIKDSVVA GFQWATKEGA LCEENMRGVR FDVHDVTLHA DAIHRGGGQI IPTARRCLYA SVLTAQPRLM EPIYLVEIQC PEQVVGGIYG VLNRKRGHVF EESQVAGTPM FVVKAYLPVN ESFGFTADLR SNTGGQAFPQ CVFDHWQILP GDPFDNSSRP SQVVAETRKR KGLKEGIPAL DNFLDKL

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

Molecular Weight

99.2 kDa

Sequence Positions

2-858aa; Full Length of Mature Protein

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

OMIM

130610

3D Structure

ModBase 3D Structure for P13639

Product Categories/Family for EEF2 recombinant protein

Epigenetics and Nuclear Signaling

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Product References and Citations for EEF2 recombinant protein

Complete sequence of the coding region of human elongation factor 2 (EF-2) by enzymatic amplification of cDNA from human ovarian granulosa cells.Rapp G., Klaudiny J., Hagendorff G., Luck M.R., Heinz K.Biol. Chem. Hoppe-Seyler 370:1071-1075(1989) Construction of a plasmid containing the complete coding region of human elongation factor 2.Hanes J., Freudenstein J., Rapp G., Scheit K.H.Biol. Chem. Hoppe-Seyler 373:201-204(1992) Ustek D., Bektas M., Cakiris A., Oku B., Bermek E.

Other Notes

Small volumes of EEF2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EEF2 purchase

MBS1213669 is a recombinant protein targeting EEF2. Also known as Recombinant Human Elongation factor 2. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for EEF2. It may not necessarily be applicable to this product.

NCBI GI #

4503483

NCBI GeneID

1938

NCBI Accession #

NP_001952.1

UniProt Primary Accession #

P13639

UniProt Secondary Accession #

Q58J86; B2RMP5; D6W618

UniProt Related Accession #

P13639

GenBank Nucleotide #

NM_001961.3

NCBI Official Full Name

elongation factor 2

NCBI Official Synonym Full Names

eukaryotic translation elongation factor 2

NCBI Official Symbol

EEF2

NCBI Official Synonym Symbols

EF2; EF-2; EEF-2; SCA26

UniProt Protein Name

Elongation factor 2

Protein Family

Elongation factor

UniProt Gene Name

EEF2

UniProt Synonym Gene Names

EF2; EF-2

UniProt Entry Name

EF2_HUMAN

NCBI Protein Information

elongation factor 2

NCBI Summary for EEF2

This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008]

UniProt Comments for EEF2

EEF2: a member of the GTP-binding translation elongation factor family. An essential factor for protein synthesis. Promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by eEF2 kinase phosphorylation. eEF2 kinase is normally dependent on Ca2+ ions and calmodulin. eEF2 kinase can also be activated by PKA in response to elevated cAMP levels, which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation.

Protein type: Translation; Translation elongation

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytoplasm; cytosol; lipid raft; membrane; nucleus; plasma membrane; polysomal ribosome; ribonucleoprotein complex

Molecular Function: 5S rRNA binding; actin filament binding; GTP binding; GTPase activity; p53 binding; protein binding; protein kinase binding; ribosome binding; translation elongation factor activity

Biological Process: aging; cellular protein metabolic process; gene expression; glial cell proliferation; hemopoietic progenitor cell differentiation; peptidyl-diphthamide biosynthetic process from peptidyl-histidine; positive regulation of translation; post-translational protein modification; response to estradiol stimulus; response to ethanol; response to fluoxetine; response to folic acid; response to hydrogen peroxide; skeletal muscle contraction; translation; translational elongation

Disease: Spinocerebellar Ataxia 26

Research Articles on EEF2

1. nuclear localization of active eEF-2 depends upon its interaction with p53, as cells lacking p53 contain less active eEF-2 in the nuclear compartment

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with EEF2 recombinant protein

Reference Product	PubMed Publications
RPS6 recombinant protein	>13 publications with EEF2 and RPS6
RPS23 recombinant protein	>1 publications with EEF2 and RPS23

Pathways associated with EEF2 recombinant protein

Products by Pathway	Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
BDNF Signaling Pathway antibodies	BDNF Signaling Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Eukaryotic Translation Elongation Pathway antibodies	Eukaryotic Translation Elongation Pathway Diagram
Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway antibodies	Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Infectious Disease Pathway antibodies	Infectious Disease Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram

Diseases associated with EEF2 recombinant protein

Disease Name	Pubmed Publications
Hypertrophy Antibodies	>23 publications with EEF2 and Hypertrophy
Carcinoma Antibodies	>17 publications with EEF2 and Carcinoma
Cardiovascular Diseases Antibodies	>13 publications with EEF2 and Cardiovascular Diseases
Adenocarcinoma Antibodies	>11 publications with EEF2 and Adenocarcinoma
Disease Models, Animal Antibodies	>10 publications with EEF2 and Disease Models, Animal
Necrosis Antibodies	>9 publications with EEF2 and Necrosis
Breast Neoplasms Antibodies	>9 publications with EEF2 and Breast Neoplasms
Heart Diseases Antibodies	>8 publications with EEF2 and Heart Diseases
Inflammation Antibodies	>8 publications with EEF2 and Inflammation
Kidney Diseases Antibodies	>5 publications with EEF2 and Kidney Diseases

Organs/Tissues associated with EEF2 recombinant protein

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>106 publications with EEF2 and Muscle
Brain Antibodies	>49 publications with EEF2 and Brain
Blood Antibodies	>40 publications with EEF2 and Blood
Heart Antibodies	>28 publications with EEF2 and Heart
Liver Antibodies	>20 publications with EEF2 and Liver
Kidney Antibodies	>12 publications with EEF2 and Kidney
Vascular Antibodies	>11 publications with EEF2 and Vascular
Lung Antibodies	>9 publications with EEF2 and Lung
Pancreas Antibodies	>7 publications with EEF2 and Pancreas
Bone Antibodies	>5 publications with EEF2 and Bone