Calpain-1 Catalytic Subunit (CAPN1) Mouse Recombinant Protein

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Poirier C., Poussard S., Faust D.M., Imaizumi-Sherrer T., Weiss M.C., Ducastaing A., Montarras D., Pinset C., Guenet J.-L. Cloning and characterization of the cDNA and gene encoding the mouse mu-calpain large subunit protein.Sahr K.E., Andrabi S., Peters L.L., Chishti A.H. Comprehensive identification of phosphorylation sites in postsynaptic density preparations.Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.Mol. Cell. Proteomics 5:914-922(2006)

Small volumes of Capn1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Recombinant Mouse Calpain-1 catalytic subunit (Capn1 or capn)

CAPN1 is the encodes the Calpain-1 catalytic subunit protein (capn)
The calpain family of calcium-activated neutral proteases have expression throughout the body. They are nonlysosomal, intracellular cysteine proteases. Also the calpains are ubiquitous enzymes that consist of heterodimers with distinct large, catalytic subunits. Although the physiological role of calpains is still poorly understood, they have been shown to be active in cell mobility and cell cycle progression, as well as cell-type specific functions such as long-term potentiation in neurons and cell fusion in myoblasts. Under these physiological conditions, a transient and localized influx of calcium into the cell activates a small local population of calpains (for example, those close to Ca2+ channels), which then advance the signal transduction pathway by catalyzing the controlled proteolysis of its target proteins. CAPN has been shown to interact with PSEN2. PSEN2 belongs to the presenilin family of multi-pass transmembrane proteins that function as a part of the gamma-secretase intramembrane protease complex. Associations of expression have been made to patients with Alzheimer’s disease.
Cross species reactivity has been demonstrated to mouse, rat and human sequences. Applications include both western blotting assay as well as ELISA kit development for target identification.