Red Clover Mottle Virus RCMVs1gp1 Recombinant Protein – Cat# MBS1230568

RNA1 Polyprotein (RCMVs1gp1) Red Clover Mottle Virus Recombinant Protein

Name: RCMVs1gp1 Recombinant Protein; Red Clover Mottle Virus; MyBioSource
Brand: MyBioSource
SKU: MBS1230568
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1230568

MBS1230568 is a recombinant protein targeting RCMVs1gp1. Also known as Recombinant Red clover mottle virus RNA1 polyprotein. Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

RNA1 polyprotein (RCMVs1gp1), Recombinant Protein

Full Product Name

Recombinant Red clover mottle virus RNA1 polyprotein

Product Gene Name

RCMVs1gp1 recombinant protein

Product Synonym Names

Recombinant RNA1 polyprotein; RNA1 polyprotein; Genome polyprotein B P1 Cleaved into the following 5 chains: 1. Protease cofactor; 32 kDa protein Putative helicase EC= 3.6.4.-; 58 kDa protein Membrane-binding protein NTP-binding protein; NTB Viral genome-

Sequence

GAEKYFNFYP IEYDAAEGIA RVGELKPKLY IPLPKKTSLV KTPEEWHLGT PCDKVPSILV KGDPRLADTV HADYDPCLSG LTKYSTPMSP LDSVLLGETC QEILDEWFDC LPEGFELGEV TINEALNGVD GVDYMDRIPL ATSEGFPHVM SREQGEKGKQ RFVQGDGHIV SLIPGTSVHE AYETLSRTIA TEVPTLVGIE CPKDEKLPFR KVFTKPKTRN FTILPMEYNI LVRQYFLNFV RFIMKKRDVL PCQVGINPYS MEWSIVASRL KSQGNDILCC DYSSFDGLLS KQIMEMMADM INRFCGGGTL ICAKRKNLLM ACCSRLAISR DSVWRIECGI PSGFPLTVIC NSIFNEILVR YHYKLLLQEH NAPNMYVQSF KNLISMVTYG DDNLISVNAV VKPYFDGTKL KQAMARNGII ITDGKDKTSA TLEFRRLEDC DFLKRGFLKR SSVLWDAPEE KASLWAQLHY VNVNNCEMQV AYMTNLVNVL RELYMHDPTE MVEFRRLALK SIPWLNTTDL PTLYQVKEFY AEQRLRNIPD HNDSLDMLTS VDLLGPAILG EGVPQEALVL SELLEVRDLR YHTVPDNDNG KEVWILFNTM YPQKLLPSNC HSFTWNCGQG RGGLPTQHWL ATNVTRTDSK LNKLIRTAVA ANKKIVLATK DNILPINVIA VLLAARNKVM PSLATNALLT YVIGAAKKLN FLTSECQFAF FNV

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

Molecular Weight

210,256 Da

Sequence Positions

1152-1864

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

Chromosome Location

NC_003741.1 (270..5864). Location: cell line: RNA isolated from virions, segment: RNA 1

3D Structure

ModBase 3D Structure for P35930

Species

Red clover mottle virus (RCMV)

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of RCMVs1gp1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RCMVs1gp1. It may not necessarily be applicable to this product.

NCBI GI #

20387442

NCBI GeneID

1502336

NCBI Accession #

NP_620468.1

UniProt Primary Accession #

P35930

UniProt Secondary Accession #

Q66182

UniProt Related Accession #

JQ1657

GenBank Nucleotide #

NC_003741.1

NCBI Official Full Name

polyprotein

NCBI Official Symbol

RCMVs1gp1

UniProt Protein Name

RNA1 polyprotein

UniProt Synonym Protein Names

Genome polyprotein B; P1Cleaved into the following 5 chains:Protease cofactorAlternative name(s):32 kDa protein

Protein Family

RNA1 polyprotein

UniProt Gene Name

NTB

UniProt Synonym Gene Names

3C-like protease

UniProt Entry Name

POL1_RCMV

NCBI Protein Information

polyprotein; protease cofactor; NTP-binding domain; VPg; RNA-dependent RNA polymerase

UniProt Comments for RCMVs1gp1

Function: Picornain 3C-like protease is a thiol protease that probably cleaves the B and M polyproteins.VPg plays a role in RNA replication

By similarity.

Catalytic activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subcellular location: Putative helicase: Host membrane; Single-pass membrane protein

Potential.

Post-translational modification: Specific enzymatic cleavages by picornain 3C-like protease in vivo yield mature proteins. Picornain 3C-like protease is autocatalytically processed

By similarity.VPg is uridylylated by the polymerase and is covalently linked to the 5'-end of genomic RNA. This uridylylated form acts as a nucleotide-peptide primer for the polymerase

By similarity.

Sequence similarities: Belongs to the comoviridae genome polyprotein B family.Contains 1 peptidase C3 domain.Contains 1 RdRp catalytic domain.Contains 1 SF3 helicase domain.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.