LEUM_0227 Recombinant Protein – Cat# MBS1236388

Energy-Coupling Factor Transporter Transmembrane Protein Ecft (Ecft) (LEUM_0227) Recombinant Protein

Name: LEUM_0227 Recombinant Protein; MyBioSource
Brand: MyBioSource
SKU: MBS1236388
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1236388

MBS1236388 is a recombinant protein targeting LEUM_0227. Also known as Recombinant Leuconostoc mesenteroides subsp. mesenteroides Energy-coupling factor transporter transmembrane protein EcfT (ecfT). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

Please Inquire

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Energy-coupling factor transporter transmembrane protein EcfT (ecfT) (LEUM_0227), Recombinant Protein

Full Product Name

Recombinant Leuconostoc mesenteroides subsp. mesenteroides Energy-coupling factor transporter transmembrane protein EcfT (ecfT)

Product Gene Name

LEUM_0227 recombinant protein

Product Synonym Gene Name

ecfT

Product Synonym Names

Recombinant Energy-coupling factor transporter transmembrane protein EcfT (ecfT); Energy-coupling factor transporter transmembrane protein EcfT; ECF transporter T component EcfT

Sequence

MNNIMIGRFV PGDSWIHRLD PRTKMIGTFI FIFVMLWSTS WATYAWSAAF VVLAIRLTKQ PFRLYWDGLK PIFWLILFTV VLQLFFTPGT PVLLHAGPLK VTIPGIINAI YVMIRFVLII LMSTILTLTT PPTSIANALE SLLKPLKKIH VPVAELSLML SIALRFVPLL MDETQKIMNA QKSRGMSFST GGPIKRAKAI VPLLIPLFVG ALQRALDLAN AMEVRGFQDA TQRTKYRVLS YGSNDRSAFI GLIGFTIIFI GINFFIK

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

Molecular Weight

30,003 Da

Sequence Positions

1-267

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

Chromosome Location

NC_008531.1 (191004..191807)

3D Structure

ModBase 3D Structure for Q03ZL4

Species

Leuconostoc mesenteroides subsp. mesenteroides (strain 8293 / NCDO 523)

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of LEUM_0227 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LEUM_0227. It may not necessarily be applicable to this product.

NCBI GI #

116617360

NCBI GeneID

4422128

NCBI Accession #

YP_817731.1

UniProt Primary Accession #

Q03ZL4

UniProt Related Accession #

ZP_00063513

GenBank Nucleotide #

NC_008531.1

NCBI Official Full Name

cobalt ABC transporter permease CbiQ

NCBI Official Symbol

LEUM_0227

UniProt Protein Name

Energy-coupling factor transporter transmembrane protein EcfT

UniProt Gene Name

ecfT

UniProt Synonym Gene Names

ECF transporter T component EcfT

UniProt Entry Name

ECFT_LEUMM

NCBI Protein Information

cobalt ABC transporter permease CbiQ

UniProt Comments for LEUM_0227

Function: Part of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates including 5-formyltetrahydrofolate, pantothenate and riboflavin. Upon expression of the complex plus FolT in E.coli allows 5-formyltetrahydrofolate uptake; 5-formyltetrahydrofolate is not taken up in the absence of FolT or the EcfA1A2T complex. Ref.2 Ref.3

Subunit structure: Forms a stable energy-coupling factor (ECF) transporter complex probably composed of a membrane-embedded substrate-binding protein (S component), two ATP-binding proteins (A components) and a transmembrane protein (T component). This complex interacts with a number of substrate-specific components, including FolT, PanT and RibU for 5-formyltetrahydrofolate, pantothenate and riboflavin respectively. Ref.2

Subcellular location: Cell membrane; Multi-pass membrane protein

Probable Ref.2 Ref.3.

Sequence similarities: Belongs to the energy-coupling factor EcfT family.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with LEUM_0227 recombinant protein

Products by Pathway	Pathway Diagram
ABC Transporters Pathway antibodies	ABC Transporters Pathway Diagram
ABC Transporters Pathway antibodies	ABC Transporters Pathway Diagram
Energy-coupling Factor Transport System Pathway antibodies	Energy-coupling Factor Transport System Pathway Diagram
Energy-coupling Factor Transport System Pathway antibodies	Energy-coupling Factor Transport System Pathway Diagram