Outer Capsid Glycoprotein VP7 Rotavirus A Recombinant Protein

Function: Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. In integrin-dependent strains, VP7 seems to essentially target the integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 at a postbinding stage, once the initial attachment by VP4 has been achieved

By similarity.

Subunit structure: Homotrimer; in the presence of calcium

By similarity. Acquisition of the capsid outer layer requires a high calcium concentration inside the endoplasmic reticulum. Following cell entry, the low calcium concentration in the cytoplasm is probably responsible for the solubilization of the outer layer. Interacts with host integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3

By similarity.

Subcellular location: Virion

By similarity. Host endoplasmic reticulum lumen

Potential. Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles

By similarity.

Post-translational modification: The N-terminus is blocked possibly by pyroglutamic acid

By similarity.N-glycosylated

By similarity.Intramolecular disulfide bonds

By similarity.

Miscellaneous: In group A rotaviruses, VP7 defines the G serotype.

Sequence similarities: Belongs to the rotavirus VP7 family.