Human NCK2 Recombinant Protein – Cat# MBS1244706

Cytoplasmic Protein NCK2 (NCK2) Human Recombinant Protein

Name: NCK2 Recombinant Protein; Cytoplasmic Protein NCK2; Human; MyBioSource
Brand: MyBioSource
SKU: MBS1244706
Price: 365 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1244706

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional… More details

$365 / 0.02 mg (Yeast) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Cytoplasmic protein NCK2, Recombinant Protein

Full Product Name

Recombinant Human Cytoplasmic protein NCK2

Product Gene Name

NCK2 recombinant protein

Product Synonym Names

Growth factor receptor-bound protein 4NCK adaptor protein 2; Nck-2SH2/SH3 adaptor protein NCK-beta

Sequence

MTEEVIVIAK WDYTAQQDQE LDIKKNERLW LLDDSKTWWR VRNAANRTGY VPSNYVERKN SLKKGSLVKN LKDTLGLGKT RRKTSARDAS PTPSTDAEYP ANGSGADRIY DLNIPAFVKF AYVAEREDEL SLVKGSRVTV MEKCSDGWWR GSYNGQIGWF PSNYVLEEVD EAAAESPSFL SLRKGASLSN GQGSRVLHVV QTLYPFSSVT EEELNFEKGE TMEVIEKPEN DPEWWKCKNA RGQVGLVPKN YVVVLSDGPA LHPAHAPQIS YTGPSSSGRF AGREWYYGNV TRHQAECALN ERGVEGDFLI RDSESSPSDF SVSLKASGKN KHFKVQLVDN VYCIGQRRFH TMDELVEHYK KAPIFTSEHG EKLYLVRALQ

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

Molecular Weight

58.9 kDa

Sequence Positions

1-380aa; Full Length

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

OMIM

604930

3D Structure

ModBase 3D Structure for O43639

Product Categories/Family for NCK2 recombinant protein

Transcription

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Product References and Citations for NCK2 recombinant protein

Identification of Nck family genes, chromosomal localization, expression, and signaling specificity.Chen M., She H., Davis E.M., Spicer C.M., Kim L., Ren R., LeBeau M.M., Li W.J. Biol. Chem. 273:25171-25178(1998) Nck-2, a novel Src homology2/3-containing adaptor protein that interacts with the LIM-only protein PINCH and components of growth factor receptor kinase-signaling pathways.Tu Y., Li F., Wu C.Mol. Biol. Cell 9:3367-3382(1998)

Other Notes

Small volumes of NCK2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NCK2 purchase

MBS1244706 is a recombinant protein targeting NCK2. Also known as Recombinant Human Cytoplasmic protein NCK2. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for NCK2. It may not necessarily be applicable to this product.

NCBI GI #

52630425

NCBI GeneID

8440

NCBI Accession #

NP_001004720.1

UniProt Primary Accession #

O43639

UniProt Secondary Accession #

Q9BWN9; Q9UIC3; D3DVK1

UniProt Related Accession #

O43639

GenBank Nucleotide #

NM_001004720.2

NCBI Official Full Name

cytoplasmic protein NCK2 isoform A

NCBI Official Synonym Full Names

NCK adaptor protein 2

NCBI Official Symbol

NCK2

NCBI Official Synonym Symbols

GRB4; NCKbeta

UniProt Protein Name

Cytoplasmic protein NCK2

UniProt Synonym Protein Names

Growth factor receptor-bound protein 4; NCK adaptor protein 2; Nck-2; SH2/SH3 adaptor protein NCK-beta

Protein Family

Cytoplasmic protein

UniProt Gene Name

NCK2

UniProt Synonym Gene Names

GRB4; Nck-2

UniProt Entry Name

NCK2_HUMAN

NCBI Protein Information

cytoplasmic protein NCK2

NCBI Summary for NCK2

This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

UniProt Comments for NCK2

NCK2: Adapter protein which associates with tyrosine- phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1- dependent transcriptional activation in response to activated Ras signaling.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; trans-Golgi network; vesicle membrane

Molecular Function: ARF guanyl-nucleotide exchange factor activity; cytoskeletal adaptor activity; protein binding; protein complex binding; receptor signaling complex scaffold activity; SH3/SH2 adaptor activity

Biological Process: actin filament organization; axon guidance; cell migration; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; formation of immunological synapse; lamellipodium biogenesis; negative regulation of cell proliferation; negative regulation of peptidyl-serine phosphorylation; positive regulation of actin filament polymerization; positive regulation of GTPase activity; positive regulation of T cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of epidermal growth factor receptor activity; signal complex assembly; signal transduction; T cell activation; vascular endothelial growth factor receptor signaling pathway; vesicle-mediated transport

Research Articles on NCK2

1. Tir-Intimin interaction recruits the Nck adaptor to a Tir tyrosine phosphorylated residue where it activates neural Wiskott-Aldrich syndrome protein (N-WASP).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NCK2 recombinant protein

Reference Product	PubMed Publications
ILK recombinant protein	>5 publications with NCK2 and ILK
LIMS1 recombinant protein	>5 publications with NCK2 and LIMS1
PAK1 recombinant protein	>4 publications with NCK2 and PAK1
PDGFRB recombinant protein	>3 publications with NCK2 and PDGFRB
EGFR recombinant protein	>2 publications with NCK2 and EGFR
PAK3 recombinant protein	>1 publications with NCK2 and PAK3

Pathways associated with NCK2 recombinant protein

Products by Pathway	Pathway Diagram
Activation Of Rac Pathway antibodies	Activation Of Rac Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
BDNF Signaling Pathway antibodies	BDNF Signaling Pathway Diagram
Cell-Cell Communication Pathway antibodies	Cell-Cell Communication Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Downstream Signal Transduction Pathway antibodies	Downstream Signal Transduction Pathway Diagram
EGFR1 Signaling Pathway antibodies	EGFR1 Signaling Pathway Diagram
EPH-Ephrin Signaling Pathway antibodies	EPH-Ephrin Signaling Pathway Diagram

Diseases associated with NCK2 recombinant protein

Disease Name	Pubmed Publications
Heart Diseases Antibodies	>1 publications with NCK2 and Heart Diseases
Vascular Diseases Antibodies	>1 publications with NCK2 and Vascular Diseases
Hypertrophy Antibodies	>1 publications with NCK2 and Hypertrophy
Kidney Diseases Antibodies	>1 publications with NCK2 and Kidney Diseases
Cell Transformation, Neoplastic Antibodies	>1 publications with NCK2 and Cell Transformation, Neoplastic
Necrosis Antibodies	>1 publications with NCK2 and Necrosis
Proteinuria Antibodies	>1 publications with NCK2 and Proteinuria

Organs/Tissues associated with NCK2 recombinant protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>6 publications with NCK2 and Brain
Kidney Antibodies	>4 publications with NCK2 and Kidney
Skin Antibodies	>4 publications with NCK2 and Skin
Intestine Antibodies	>3 publications with NCK2 and Intestine
Heart Antibodies	>3 publications with NCK2 and Heart
Embryonic Tissue Antibodies	>2 publications with NCK2 and Embryonic Tissue
Bone Antibodies	>2 publications with NCK2 and Bone
Mammary Gland Antibodies	>1 publications with NCK2 and Mammary Gland
Lung Antibodies	>1 publications with NCK2 and Lung
Uterus Antibodies	>1 publications with NCK2 and Uterus