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Delta (14)-Sterol Reductase (ERG24) Saccharomyces Cerevisiae Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1246969

MBS1246969 is a recombinant protein targeting ERG24. Also known as Recombinant Saccharomyces cerevisiae Delta (14)-sterol reductase. Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Delta (14)-sterol reductase (ERG24), Recombinant Protein
Full Product Name
Recombinant Saccharomyces cerevisiae Delta (14)-sterol reductase
Product Gene Name
Product Synonym Gene Name
ERG24
Product Synonym Names
Recombinant Delta (14)-sterol reductase; Delta(14)-sterol reductase EC= 1.3.1.70; C-14 sterol reductase Sterol C14-reductase
Sequence
MVSALNPRTT EFEFGGLIGA LGISIGLPVF TIILNQMIRP DYFIKGFFQN FDIVELWNGI KPLRYYLGNR ELWTVYCLWY GILAVLDVIL PGRVMKGVQL RDGSKLSYKI NGIAMSTTLV LVLAIRWKLT DGQLPELQYL YENHVSLCII SILFSFFLAT YCYVASFIPL IFKKNGNGKR EKILALGGNS GNIIYDWFIG RELNPRLGPL DIKMFSELRP GMLLWLLINL SCLHHHYLKT GKINDALVLV NFLQGFYIFD GVLNEEGVLT MMDITTDGFG FMLAFGDLSL VPFTYSLQAR YLSVSPVELG WVKVVGILAI MFLGFHIFHS ANKQKSEFRQ GKLENLKSIQ TKRGTKLLCD GWWAKSQHIN YFGDWLISLS WCLATWFQTP LTYYYSLYFA TLLLHRQQRD EHKCRLKYGE NWEEYERKVP YKIIPYVY
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C. For extended storage, store at -20 or -80 degree C.
Molecular Weight
50,615 Da
Sequence Positions
1-438
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Chromosome Location
Chromosome: XIV; NC_001146.8 (109095..110411, complement). Location: chromosome: XIV
3D Structure
ModBase 3D Structure for P32462
Species
Saccharomyces cerevisiae (strain 204508 / S288c) (Baker's yeast)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ERG24 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ERG24. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Erg24p
NCBI Official Symbol
ERG24
UniProt Protein Name
Delta(14)-sterol reductase
UniProt Synonym Protein Names
C-14 sterol reductase; Sterol C14-reductase
UniProt Gene Name
ERG24
UniProt Entry Name
ERG24_YEAST
NCBI Protein Information
Erg24p

UniProt Comments for ERG24

Function: Reduces the C14=C15 double bond of 4,4-dimethyl-cholesta-8,14,24-trienol to produce 4,4-dimethyl-cholesta-8,24-dienol.

Catalytic activity: 4,4-dimethyl-5-alpha-cholesta-8,24-dien-3-beta-ol + NADP+ = 4,4-dimethyl-5-alpha-cholesta-8,14,24-trien-3-beta-ol + NADPH.

Enzyme regulation: Inhibited by the morpholine antifungal drug fenpropimorph.

Pathway: Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 2/6.

Subcellular location: Membrane; Multi-pass membrane protein.

Miscellaneous: Present with 1600 molecules/cell in log phase SD medium.

Sequence similarities: Belongs to the ERG4/ERG24 family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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