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ATP2A2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS125014
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This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into… More details

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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ATP2A2/SERCA2, Polyclonal Antibody
Full Product Name
ATP2A2(SERCA2) Rabbit pab Antibody
Product Gene Name
Product Synonym Names
ATP2B;DAR;DD;SERCA2;ATP2A2/SERCA2;ATP2A2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Liquid; PBS with 0.1% Sodium Azide, 50% Glycerol,
Purity/Purification
Affinity purification
Preparation and Storage
Upon receipt store at -20 degree C. Avoid freeze/thaw cycles.
Molecular Weight
Observed MW: 114kDa
Calculated MW: 109kDa/111kDa/114kDa
Chromosome Location
Chromosome: 12; NC_000012.11 (110719032..110788898). Location: 12q24.11
OMIM
101900
3D Structure
ModBase 3D Structure for P16615
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 111-253 of human SERCA2/ATP2A2.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ATP2A2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ATP2A2 antibody
Western Blot (WB): 1/500-1/2000
IHC: 1/50-1/100

Western Blot (WB) of anti-ATP2A2 antibody

Western blot analysis of extracts of various cell lines, using ATP2A2 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Rabbit Anti-ATP2A2 Polyclonal Antibody – Cat# MBS125014

Western Blot (WB) of anti-ATP2A2 antibody

Western blot analysis of extracts of Mouse heart, using SERCA2/ATP2A2 antibody (A0098) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Rabbit Anti-ATP2A2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS125014

Immunohistochemistry (IHC) of anti-ATP2A2 antibody

Immunohistochemistry of paraffin-embedded rat heart using SERCA2/ATP2A2 antibody (MBS125014) at dilution of 1:100 (40xlens).
Rabbit Anti-ATP2A2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS125014

Immunohistochemistry (IHC) of anti-ATP2A2 antibody

mmunohistochemistry of paraffin-embedded mouse lung using SERCA2/ATP2A2 antibody (MBS125014) at dilution of 1:100 (40x lens).
Rabbit Anti-ATP2A2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS125014

Immunohistochemistry (IHC) of anti-ATP2A2 antibody

mmunohistochemistry of paraffin-embedded mouse heart using SERCA2/ATP2A2 antibody (MBS125014) at dilution of 1:100 (40x lens).
Rabbit Anti-ATP2A2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS125014

Product References and Citations for anti-ATP2A2 antibody

1. Vangheluwe, P. et al. (2005) Cell Calcium 38, 291-302. 2. de Smedt, H. et al. (1991) J Biol Chem 266, 7092-5. 3. Gélébart, P. et al. (2003) Biochem Biophys Res Commun 303, 676-84. 4. Hawkins, C. et al. (1995) Mol Cell Biochem 142, 131-8. 5. Viner, R.I. et al. (1999) Biochem J 340 (Pt 3), 657-69. 6. Sakuntabhai, A. et al. (1999) Hum Mol Genet 8, 1611-9.

Other Notes

Small volumes of anti-ATP2A2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATP2A2 purchase

MBS125014 is an antibody targeting ATP2A2. Also known as ATP2A2(SERCA2) Rabbit pab Antibody. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATP2A2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform a
NCBI Official Synonym Full Names
ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
NCBI Official Symbol
ATP2A2
NCBI Official Synonym Symbols
DD; DAR; ATP2B; SERCA2
UniProt Protein Name
Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
UniProt Synonym Protein Names
Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase
UniProt Gene Name
ATP2A2
UniProt Synonym Gene Names
ATP2B; SERCA2
UniProt Entry Name
AT2A2_HUMAN
NCBI Protein Information
sarcoplasmic/endoplasmic reticulum calcium ATPase 2; calcium pump 2; SR Ca(2+)-ATPase 2; cardiac Ca2+ ATPase; endoplasmic reticulum class 1/2 Ca(2+) ATPase; ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2; calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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