Rabbit Anti-NCK1 Polyclonal Antibody – Cat# MBS125034

Western blot analysis of extracts of various cell lines, using NCK1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

NCK1 Antibody – Rabbit Polyclonal

Name: NCK1 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS125034
Price: 360 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS125034

NCK family adaptor proteins function to couple tyrosine phosphorylation signals to regulate actin cytoskeletal reorganization that leads to cell motility. Cytoplasmic protein NCK1 (or NCK alpha) is an adapter protein which associates with… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

NCK1, Polyclonal Antibody

Full Product Name

NCK1 Polyclonal Antibody

Product Gene Name

anti-NCK1 antibody

Product Synonym Names

NCK; nck-1; NCKalpha

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Purity/Purification

Affinity Purification

Concentration

1mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

377

OMIM

600508

3D Structure

ModBase 3D Structure for P16333

Species

Human

Immunogen

Recombinant protein of human NCK1

Calculated Molecular Weight

43kDa

Product Categories/Family for anti-NCK1 antibody

Polyclonal

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-NCK1 antibody

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Application Notes for anti-NCK1 antibody

WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:20 - 1:50

Other Notes

Small volumes of anti-NCK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NCK1 purchase

MBS125034 is an antibody targeting NCK1. Also known as NCK1 Polyclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NCK1. It may not necessarily be applicable to this product.

NCBI GI #

127962

NCBI GeneID

4690

NCBI Accession #

P16333.1

UniProt Primary Accession #

P16333

UniProt Secondary Accession #

B7Z751; D3DNE3

UniProt Related Accession #

P16333

NCBI Official Full Name

Cytoplasmic protein NCK1

NCBI Official Synonym Full Names

NCK adaptor protein 1

NCBI Official Symbol

NCK1

NCBI Official Synonym Symbols

NCK; nck-1; NCKalpha

UniProt Protein Name

Cytoplasmic protein NCK1

UniProt Synonym Protein Names

NCK adaptor protein 1; Nck-1; SH2/SH3 adaptor protein NCK-alpha

Protein Family

Cytoplasmic protein

UniProt Gene Name

NCK1

UniProt Synonym Gene Names

NCK; Nck-1

UniProt Entry Name

NCK1_HUMAN

NCBI Protein Information

cytoplasmic protein NCK1; NCK tyrosine kinase; melanoma NCK protein; SH2/SH3 adaptor protein NCK-alpha; non-catalytic region of tyrosine kinase

NCBI Summary for NCK1

The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010]

UniProt Comments for NCK1

NCK1: adapter protein containing Src homology 2 and 3 (SH2 and SH3) domains. Transduces signals from tyrosine-phosphorylated growth factor receptors to their cellular substrates.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: endoplasmic reticulum; cytoplasm; plasma membrane; ribosome; protein phosphatase type 1 complex; intercellular junction; vesicle membrane; cytosol; nucleus

Molecular Function: protein binding, bridging; protein domain specific binding; protein binding; receptor signaling complex scaffold activity; protein kinase inhibitor activity; SH3/SH2 adaptor activity; cytoskeletal adaptor activity; receptor tyrosine kinase binding; receptor binding

Biological Process: lamellipodium biogenesis; axon guidance; positive regulation of translation in response to stress; T cell activation; negative regulation of peptidyl-serine phosphorylation; signal complex assembly; actin filament organization; response to other organism; T cell receptor signaling pathway; substrate-bound cell migration, cell extension; positive regulation of actin filament polymerization; negative regulation of protein kinase activity; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of T cell proliferation; vascular endothelial growth factor receptor signaling pathway

Research Articles on NCK1

1. the present study characterized a novel Nck-1-ELMO1 interaction and defined a new role for Nck-1 in regulating Rac1 activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NCK1 antibody

Reference Product	PubMed Publications
EGFR antibody	>2 publications with NCK1 and EGFR
PDGFRB antibody	>2 publications with NCK1 and PDGFRB
RASA1 antibody	>1 publications with NCK1 and RASA1
PAK1 antibody	>1 publications with NCK1 and PAK1
CBL antibody	>1 publications with NCK1 and CBL

Pathways associated with anti-NCK1 antibody

Products by Pathway	Pathway Diagram
Activation Of Rac Pathway antibodies	Activation Of Rac Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies	Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway antibodies	Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway Diagram
Arf6 Signaling Events Pathway antibodies	Arf6 Signaling Events Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
BDNF Signaling Pathway antibodies	BDNF Signaling Pathway Diagram

Diseases associated with anti-NCK1 antibody

Disease Name	Pubmed Publications
Carcinoma, Hepatocellular Antibodies	>5 publications with NCK1 and Carcinoma, Hepatocellular
Liver Diseases Antibodies	>4 publications with NCK1 and Liver Diseases
Breast Neoplasms Antibodies	>3 publications with NCK1 and Breast Neoplasms
Kidney Diseases Antibodies	>2 publications with NCK1 and Kidney Diseases
Hematologic Diseases Antibodies	>2 publications with NCK1 and Hematologic Diseases
Nervous System Diseases Antibodies	>2 publications with NCK1 and Nervous System Diseases
Neoplasm Metastasis Antibodies	>2 publications with NCK1 and Neoplasm Metastasis
Proteinuria Antibodies	>2 publications with NCK1 and Proteinuria
Nerve Degeneration Antibodies	>1 publications with NCK1 and Nerve Degeneration
Carcinoma, Squamous Cell Antibodies	>1 publications with NCK1 and Carcinoma, Squamous Cell

Organs/Tissues associated with anti-NCK1 antibody

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>1 publications with NCK1 and Eye
Pancreas Antibodies	>1 publications with NCK1 and Pancreas
Lung Antibodies	>1 publications with NCK1 and Lung