Bacillus Subtilis glpG Recombinant Protein – Cat# MBS1250736

Rhomboid Protease Glup (Glup) (glpG) Bacillus Subtilis Recombinant Protein

Name: glpG Recombinant Protein; Bacillus Subtilis; MyBioSource
Brand: MyBioSource
SKU: MBS1250736
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1250736

MBS1250736 is a recombinant protein targeting glpG. Also known as Recombinant Bacillus subtilis Rhomboid protease gluP (gluP). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Rhomboid protease gluP (gluP) (glpG), Recombinant Protein

Full Product Name

Recombinant Bacillus subtilis Rhomboid protease gluP (gluP)

Product Gene Name

glpG recombinant protein

Product Synonym Gene Name

gluP; yqgP

Product Synonym Names

Recombinant Rhomboid protease gluP (gluP); Rhomboid protease gluP EC= 3.4.21.105; Intramembrane serine protease

Sequence

MFLLEYTYWK IAAHLVNSGY GVIQAGESDE IWLEAPDKSS HDLVRLYKHD LDFRQEMVRD IEEQAERVER VRHQLGRRRM KLLNVFFSTE APVDDWEEIA KKTFEKGTVS VEPAIVRGTM LRDDLQAVFP SFRTEDCSEE HASFENAQMA RERFLSLVLK QEEQRKTEAA VFQNGKPTFT YLFIALQILM FFLLEINGGS TNTETLVAFG AKENSLIAQG EWWRLLTPIV LHIGIAHLAF NTLALWSVGT AVERMYGSGR FLLIYLAAGI TGSIASFVFS PYPSAGASGA IFGCLGALLY VALSNRKMFL RTIGTNIIVI IIINLGFGFA VSNIDNSGHI GGLIGGFFAA AALGLPKAGA FGKRLLSAVL LIALAVGFLY YGLHSPSHQE SALIQQASEL YQEGKYEEVT ELLNGEAAQK DASADLLKIL AVSDIQIGEY DQAVSLLERA VKKEPKDHAS YYNLALLYAE KNELAQAEKA IQTAVKLKPK EQRYKELQRQ IENNKES

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

Molecular Weight

56,462 Da

Sequence Positions

1-507

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

Chromosome Location

NC_000964.3 (2571907..2573430, complement)

3D Structure

ModBase 3D Structure for P54493

Species

Bacillus subtilis (strain 168)

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of glpG recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for glpG. It may not necessarily be applicable to this product.

NCBI GI #

255767560

NCBI GeneID

938211

NCBI Accession #

NP_390367.2

UniProt Primary Accession #

P54493

UniProt Related Accession #

P54493

GenBank Nucleotide #

NC_000964.3

NCBI Official Full Name

membrane endopeptidase

NCBI Official Symbol

glpG

UniProt Protein Name

Rhomboid protease GluP

UniProt Synonym Protein Names

Intramembrane serine protease

Protein Family

Rhomboid protease

UniProt Gene Name

gluP

UniProt Synonym Gene Names

yqgP

UniProt Entry Name

GLUP_BACSU

NCBI Protein Information

membrane endopeptidase

UniProt Comments for glpG

Function: Rhomboid-type serine protease that catalyzes intramembrane proteolysis. Important for normal cell division and sporulation. May act as a glucose exporter. Ref.4 Ref.5 Ref.6 Ref.7

Catalytic activity: Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.

Enzyme regulation: Inhibited by dichloroisocoumarin (DCI) and N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), but not by other serine protease inhibitors such as sulfonyl fluoride PMSF and 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF). Ref.6

Subcellular location: Cell membrane; Multi-pass membrane protein Ref.5.

Sequence similarities: Belongs to the peptidase S54 family.Contains 2 TPR repeats.

Biophysicochemical propertiesTemperature dependence:Is more active at more active at 25 degrees Celsius than at 37 degrees Celsius.

Research Articles on glpG

1. A paper described the role of YqgP as a glucose exporter. YqgP is renamed to GluP that stands for glucose transporter protein.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with glpG recombinant protein

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