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MMP9 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS125192
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Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MMP9, Polyclonal Antibody
Full Product Name
MMP9 Polyclonal Antibody
Product Gene Name
anti-MMP9 antibody
Product Synonym Names
GELB; CLG4B; MMP-9; MANDP2; MMP9
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Purity/Purification
Affinity purification
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.|Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Molecular Weight
Observed MW: 96kDa
Calculated MW: 78kDa
Chromosome Location
Chromosome: 20; NC_000020.10 (44637547..44645200). Location: 20q11.2-q13.1
OMIM
120361
3D Structure
ModBase 3D Structure for P14780
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 538-707 of human MMP9 (NP_004985.2).
Species Reactivity/Cross-Reactivity
Human, Mouse, Rat
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MMP9 antibody
Western Blot (WB), Immunohistochemistry-Parqaffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-MMP9 antibody
WB: 1:500-1:1000
IHC-P: 1:50-1:200
IF/ICC: 1:50-1:200

Western Blot (WB) of anti-MMP9 antibody

Western blot analysis of extracts of various cell lines, using MMP9 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ( MBS128200 ) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Rabbit Anti-MMP9 Polyclonal Antibody – Cat# MBS125192

Western Blot (WB) of anti-MMP9 antibody

Western blot analysis of extracts of Mouse brain, using MMP9 antibody (MBS125192) at 1:710 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 60s.
Rabbit Anti-MMP9 Polyclonal Antibody, Western Blot (WB) – Cat# MBS125192

Western Blot (WB) of anti-MMP9 antibody

Western blot analysis of lysates from K-562 cells, using MMP9 Rabbit pAb (MBS125192) at 1:600 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) ( MBS128200 ) at 1:10000 dilution.
Lysates/proteins: 25?g per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Rabbit Anti-MMP9 Polyclonal Antibody, Western Blot (WB) – Cat# MBS125192

Immunohistochemistry (IHC) of anti-MMP9 antibody

Immunohistochemistry of paraffin-embedded rat spleen using MMP9 antibody (MBS125192) at dilution of 1:100 (40xlens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol
Rabbit Anti-MMP9 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS125192

Immunohistochemistry (IHC) of anti-MMP9 antibody

Immunohistochemistry of paraffin-embedded mouse testis using MMP9 antibody (MBS125192) at dilution of 1:100 (40xlens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Rabbit Anti-MMP9 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS125192

Immunohistochemistry (IHC) of anti-MMP9 antibody

Immunohistochemistry of paraffin-embedded mouse spleen using MMP9 antibody (MBS125192) at dilution of 1:100 (40xlens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Rabbit Anti-MMP9 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS125192

Immunofluorescence (IF) of anti-MMP9 antibody

Immunofluorescence analysis of L929 cells using MMP9 Rabbit pAb (MBS125192) at dilution of 1:100.
Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) ( MBS128190 ) at 1:500 dilution. Blue: DAPI for nuclear staining.
Rabbit Anti-MMP9 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS125192

Product References and Citations for anti-MMP9 antibody

1. McCawley, L.J. and Matrisian, L.M. (2001) Curr. Opin. Cell Biol. 13, 534-540. 2. Coussens, L.M. et al. (2002) Science 295, 2387-2391. 3. Sternlicht, M.D. et al. (1999) Cell 98, 137-146. 4. Vu, T.H. et al. (1998) Cell 93, 411-422. 5. Nagase, H. et al. (1990) Biochemistry 29, 5783-5789.

Other Notes

Small volumes of anti-MMP9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MMP9 purchase

MBS125192 is an antibody targeting MMP9. Also known as MMP9 Polyclonal Antibody. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MMP9. It may not necessarily be applicable to this product.

NCBI GI #
74272287
NCBI GeneID
4318
NCBI Accession #
NP_004985.2
UniProt Primary Accession #
P14780
UniProt Secondary Accession #
Q3LR70; Q8N725; Q9H4Z1; Q9UCJ9; Q9UCL1; Q9UDK2; B2R7V9
UniProt Related Accession #
P14780
GenBank Nucleotide #
NM_004994.2
NCBI Official Full Name
matrix metalloproteinase-9 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
NCBI Official Symbol
MMP9
NCBI Official Synonym Symbols
GELB; CLG4B; MMP-9; MANDP2
UniProt Protein Name
Matrix metalloproteinase-9
UniProt Synonym Protein Names
92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B
Protein Family
Matrix metalloproteinase
UniProt Gene Name
MMP9
UniProt Synonym Gene Names
CLG4B; MMP-9; GELB
UniProt Entry Name
MMP9_HUMAN
NCBI Protein Information
matrix metalloproteinase-9; 92 kDa gelatinase; type V collagenase; macrophage gelatinase; 92 kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MMP9 antibody

 Reference Product  PubMed Publications
 JUN antibody  >251 publications with MMP9 and JUN
 TIMP1 antibody  >198 publications with MMP9 and TIMP1
 SRC antibody  >28 publications with MMP9 and SRC
 THBS1 antibody  >16 publications with MMP9 and THBS1
 PLAU antibody  >11 publications with MMP9 and PLAU
 LCN2 antibody  >10 publications with MMP9 and LCN2
 ELN antibody  >7 publications with MMP9 and ELN
 CDH2 antibody  >6 publications with MMP9 and CDH2
 PLG antibody  >5 publications with MMP9 and PLG
 FOSL1 antibody  >1 publications with MMP9 and FOSL1

Pathways associated with anti-MMP9 antibody

 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 Angiogenesis Pathway antibodies  Angiogenesis Pathway Diagram
 Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies  Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Collagen Formation Pathway antibodies  Collagen Formation Pathway Diagram
 Degradation Of Collagen Pathway antibodies  Degradation Of Collagen Pathway Diagram
 Degradation Of Collagen Pathway antibodies  Degradation Of Collagen Pathway Diagram

Diseases associated with anti-MMP9 antibody

 Disease Name  Pubmed Publications
 Neoplasm Invasiveness Antibodies  >237 publications with MMP9 and Neoplasm Invasiveness
 Neoplasm Metastasis Antibodies  >218 publications with MMP9 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >106 publications with MMP9 and Breast Neoplasms
 Liver Diseases Antibodies  >101 publications with MMP9 and Liver Diseases
 Aortic Diseases Antibodies  >74 publications with MMP9 and Aortic Diseases
 Aortic Aneurysm Antibodies  >66 publications with MMP9 and Aortic Aneurysm
 Carcinoma, Squamous Cell Antibodies  >57 publications with MMP9 and Carcinoma, Squamous Cell
 Aortic Aneurysm, Abdominal Antibodies  >55 publications with MMP9 and Aortic Aneurysm, Abdominal
 Brain Ischemia Antibodies  >52 publications with MMP9 and Brain Ischemia
 Myocardial Infarction Antibodies  >46 publications with MMP9 and Myocardial Infarction

Organs/Tissues associated with anti-MMP9 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >572 publications with MMP9 and Blood
 Lung Antibodies  >257 publications with MMP9 and Lung
 Bone Antibodies  >250 publications with MMP9 and Bone
 Brain Antibodies  >184 publications with MMP9 and Brain
 Liver Antibodies  >146 publications with MMP9 and Liver
 Bone Marrow Antibodies  >107 publications with MMP9 and Bone Marrow
 Connective Tissue Antibodies  >95 publications with MMP9 and Connective Tissue
 Kidney Antibodies  >91 publications with MMP9 and Kidney
 Embryonic Tissue Antibodies  >79 publications with MMP9 and Embryonic Tissue
 Lymph Node Antibodies  >72 publications with MMP9 and Lymph Node
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