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BMP2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS125347
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This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
BMP2, Polyclonal Antibody
Full Product Name
BMP2 Polyclonal Antibody
Product Gene Name
Product Synonym Names
bmp2; BMP 2A; BMP 2; BMP2A; bone morphogenetic protein 2; Bone morphogenetic protein 2A
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.|Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
OMIM
112261
3D Structure
ModBase 3D Structure for P12643
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 283-396 of human BMP2 (NP_001191.1)
Calculated MW
44kDa
Observed MW
45 kDa

Product Categories/Family for anti-BMP2 antibody

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-BMP2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immuofluorescence (IF)
Application Notes for anti-BMP2 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF 1:50-1:200

Western Blot (WB) of anti-BMP2 antibody

Western blot analysis of extracts of various cell lines, using BMP2 Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Rabbit Anti-BMP2 Polyclonal Antibody – Cat# MBS125347

Immunohistochemistry (IHC) of anti-BMP2 antibody

Immunohistochemistry of paraffin-embedded human colon using BMP2 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-BMP2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS125347

Immunohistochemistry (IHC) of anti-BMP2 antibody

Immunohistochemistry of paraffin-embedded mouse lung using BMP2 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-BMP2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS125347

Immunofluorescence (IF) of anti-BMP2 antibody

Immunofluorescence analysis of U2OS cells using BMP2 antibody (MBS125347) at dilution of 1:100. Blue: DAPI for nuclear staining
Rabbit Anti-BMP2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS125347

Other Notes

Small volumes of anti-BMP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BMP2 purchase

MBS125347 is an antibody targeting BMP2. Also known as BMP2 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BMP2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
Bone morphogenetic protein 2
NCBI Official Synonym Full Names
bone morphogenetic protein 2
NCBI Official Symbol
BMP2
NCBI Official Synonym Symbols
BDA2; BMP2A
UniProt Protein Name
Bone morphogenetic protein 2
UniProt Synonym Protein Names
Bone morphogenetic protein 2A; BMP-2A
UniProt Gene Name
BMP2
UniProt Synonym Gene Names
BMP2A; BMP-2; BMP-2A
UniProt Entry Name
BMP2_HUMAN
NCBI Protein Information
bone morphogenetic protein 2; BMP-2A; bone morphogenetic protein 2A

NCBI Summary for BMP2

The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq, Jul 2008]

UniProt Comments for BMP2

Induces cartilage and bone formation (PubMed:3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed:24362451).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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