Rabbit Anti-PBX1 Polyclonal Antibody – Cat# MBS125391

Western blot analysis of extracts of various cell lines, using PBX1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

PBX1 Antibody – Rabbit Polyclonal

Name: PBX1 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS125391
Price: 225 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS125391

This gene encodes a nuclear protein that belongs to the PBX homeobox family of transcriptional factors. Studies in mice suggest that this gene may be involved in the regulation of osteogenesis, and required for skeletal patterning and programming. A… More details

$225 / 0.02 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PBX1, Polyclonal Antibody

Full Product Name

PBX1 Polyclonal Antibody

Product Gene Name

anti-PBX1 antibody

Product Synonym Names

Pbx1: PBX1

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Purity/Purification

Affinity Purification

Concentration

1mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

430

OMIM

176310

3D Structure

ModBase 3D Structure for P40424

Species

Human

Immunogen

Recombinant protein of human PBX1

Calculated Molecular Weight

47kDa

Product Categories/Family for anti-PBX1 antibody

Polyclonal

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-PBX1 antibody

Western Blot (WB)

Application Notes for anti-PBX1 antibody

WB: 1:500 - 1:2000

Other Notes

Small volumes of anti-PBX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PBX1 purchase

MBS125391 is an antibody targeting PBX1. Also known as PBX1 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PBX1. It may not necessarily be applicable to this product.

NCBI GI #

730279

NCBI GeneID

5087

NCBI Accession #

P40424.1

UniProt Primary Accession #

P40424

UniProt Secondary Accession #

Q5T488; B4DSC1; F5H4U9

UniProt Related Accession #

P40424

NCBI Official Full Name

Pre-B-cell leukemia transcription factor 1

NCBI Official Synonym Full Names

pre-B-cell leukemia homeobox 1

NCBI Official Symbol

PBX1

UniProt Protein Name

Pre-B-cell leukemia transcription factor 1

UniProt Synonym Protein Names

Homeobox protein PBX1; Homeobox protein PRL

Protein Family

Pre-B-cell leukemia transcription factor

UniProt Gene Name

PBX1

UniProt Synonym Gene Names

PRL

UniProt Entry Name

PBX1_HUMAN

NCBI Protein Information

pre-B-cell leukemia transcription factor 1; homeobox protein PRL; homeobox protein PBX1

UniProt Comments for PBX1

PBX1: Binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development and differentiation. Forms a heterodimer with MEIS1 which binds DNA including a cAMP-responsive sequence in CYP17. Also forms heterotrimers with MEIS1 and a number of HOX proteins including HOXA9, HOXD4, HOXD9 and HOXD10. Interacts with PBXIP1. Expressed in all tissues except in cells of the B and T lineage. Belongs to the TALE/PBX homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding; Oncoprotein; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; transcription factor binding; transcription factor activity

Biological Process: spleen development; transcription, DNA-dependent; thymus development; embryonic hemopoiesis; somatic stem cell maintenance; adrenal gland development; negative regulation of transcription factor activity; embryonic skeletal development; anterior/posterior pattern formation; negative regulation of neuron differentiation; ureteric bud branching; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of ossification; steroid biosynthetic process; sex differentiation; proximal/distal pattern formation; embryonic limb morphogenesis

Research Articles on PBX1

1. E2A-PBX1 fusion gene caused by t(1;19)(q23;p13) may be a common genetic change in AIS and a survival determinant for female AIS patients at early stage.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PBX1 antibody

Reference Product	PubMed Publications
MEIS1 antibody	>64 publications with PBX1 and MEIS1
HOXB1 antibody	>30 publications with PBX1 and HOXB1
HOXA9 antibody	>29 publications with PBX1 and HOXA9
MEIS2 antibody	>16 publications with PBX1 and MEIS2
HOXA10 antibody	>12 publications with PBX1 and HOXA10
HOXB7 antibody	>9 publications with PBX1 and HOXB7
PBXIP1 antibody	>4 publications with PBX1 and PBXIP1
ZBTB16 antibody	>3 publications with PBX1 and ZBTB16
PRL antibody	>1 publications with PBX1 and PRL

Pathways associated with anti-PBX1 antibody

Products by Pathway	Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Glucocorticoid Receptor Regulatory Network Pathway antibodies	Glucocorticoid Receptor Regulatory Network Pathway Diagram
SIDS Susceptibility Pathways antibodies	SIDS Susceptibility Pathways Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram
Transcriptional Regulation Of Pluripotent Stem Cells Pathway antibodies	Transcriptional Regulation Of Pluripotent Stem Cells Pathway Diagram

Diseases associated with anti-PBX1 antibody

Disease Name	Pubmed Publications
Cell Transformation, Neoplastic Antibodies	>37 publications with PBX1 and Cell Transformation, Neoplastic
Neoplasms, Experimental Antibodies	>34 publications with PBX1 and Neoplasms, Experimental
Burkitt Lymphoma Antibodies	>22 publications with PBX1 and Burkitt Lymphoma
Breast Neoplasms Antibodies	>8 publications with PBX1 and Breast Neoplasms
Adenocarcinoma Antibodies	>7 publications with PBX1 and Adenocarcinoma
Kidney Diseases Antibodies	>5 publications with PBX1 and Kidney Diseases
Lung Neoplasms Antibodies	>5 publications with PBX1 and Lung Neoplasms
Necrosis Antibodies	>5 publications with PBX1 and Necrosis
Inflammation Antibodies	>4 publications with PBX1 and Inflammation
Fibrosis Antibodies	>4 publications with PBX1 and Fibrosis

Organs/Tissues associated with anti-PBX1 antibody

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>96 publications with PBX1 and Bone
Brain Antibodies	>37 publications with PBX1 and Brain
Embryonic Tissue Antibodies	>35 publications with PBX1 and Embryonic Tissue
Pancreas Antibodies	>20 publications with PBX1 and Pancreas
Nerve Antibodies	>17 publications with PBX1 and Nerve
Liver Antibodies	>17 publications with PBX1 and Liver
Muscle Antibodies	>16 publications with PBX1 and Muscle
Lung Antibodies	>12 publications with PBX1 and Lung
Kidney Antibodies	>11 publications with PBX1 and Kidney
Adrenal Gland Antibodies	>9 publications with PBX1 and Adrenal Gland