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RAD50 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS125532
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The DNA repair protein Rad50 is a member of the structural maintenance of chromosomes family (SMC) and plays an important role in cell cycle checkpoint signaling and doublestrand break repair in response to DNA damage (1-4). Rad50 forms a complex with Mre11… More details

$225 / 0.02 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Rad50, Polyclonal Antibody
Full Product Name
Rad50 Polyclonal Antibody
Product Gene Name
Product Synonym Names
RAD50; RAD50; NBSLD; RAD502; hRad50; DNA repair protein RAD50;
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
Affinity purification
Preparation and Storage
Store at -20 degree C or -80 degree C. Avoid freeze / thaw cycles
Molecular Weight
150kDa
Chromosome Location
Chromosome: 5; NC_000005.9 (131892616..131980313). Location: 5q31
OMIM
604040
3D Structure
ModBase 3D Structure for Q92878
Immunogen
A synthetic -peptide of human Rad50
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-RAD50 antibody
Western Blot (WB)
Application Notes for anti-RAD50 antibody
Western Blot (WB): 1:1000-1: 2000

Western Blot (WB) of anti-RAD50 antibody

Western blot analysis of extracts of various cell lines, using RAD50 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.
Rabbit Anti-RAD50 Polyclonal Antibody – Cat# MBS125532

Immunohistochemistry (IHC) of anti-RAD50 antibody

Immunohistochemistry of paraffin-embedded human uterine cancer using RAD50 Antibody at dilution of 1:200 (40x lens).
Rabbit Anti-RAD50 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS125532

Immunohistochemistry (IHC) of anti-RAD50 antibody

Immunohistochemistry of paraffin-embedded human gastric cancer using RAD50 Antibody at dilution of 1:200 (40x lens).
Rabbit Anti-RAD50 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS125532

Immunohistochemistry (IHC) of anti-RAD50 antibody

Immunohistochemistry of paraffin-embedded human breast cancer using RAD50 Antibody at dilution of 1:100 (40x lens).
Rabbit Anti-RAD50 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS125532

Immunohistochemistry (IHC) of anti-RAD50 antibody

Immunohistochemistry of paraffin-embedded human stomach using RAD50 Antibody at dilution of 1:100 (40x lens).
Rabbit Anti-RAD50 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS125532

Immunohistochemistry (IHC) of anti-RAD50 antibody

Immunohistochemistry of paraffin-embedded human gastric cancer using RAD50 Antibody at dilution of 1:100 (40x lens).
Rabbit Anti-RAD50 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS125532

Product References and Citations for anti-RAD50 antibody

1. de Jager, M. et al. (2001) Mol. Cell 8, 1129-1135. 2. Elledge, S.J. (1996) Science 274, 1664-1672. 3. Lee, J.H. and Lim, D.S. (2006) FEBS J. 273, 1630-1636. 4. Teng, S.C. et al. (2006) J Mol Histol 37, 29-39.

Other Notes

Small volumes of anti-RAD50 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RAD50 purchase

MBS125532 is an antibody targeting RAD50. Also known as Rad50 Polyclonal Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RAD50. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA repair protein RAD50
NCBI Official Synonym Full Names
RAD50 homolog (S. cerevisiae)
NCBI Official Symbol
RAD50
NCBI Official Synonym Symbols
NBSLD; RAD502; hRad50
UniProt Protein Name
DNA repair protein RAD50
UniProt Gene Name
RAD50
UniProt Synonym Gene Names
hRAD50
UniProt Entry Name
RAD50_HUMAN
NCBI Protein Information
DNA repair protein RAD50

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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