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Pre-Glycoprotein Polyprotein Gp Complex (GPC) Lassa Virus Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1255586

MBS1255586 is a recombinant protein targeting GPC. Also known as Recombinant Lassa virus Pre-glycoprotein polyprotein GP complex (GPC), partial. Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$405 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Pre-glycoprotein polyprotein GP complex (GPC), Recombinant Protein
Full Product Name
Recombinant Lassa virus Pre-glycoprotein polyprotein GP complex (GPC), partial
Product Gene Name
GPC recombinant protein
Product Synonym Gene Name
GPC; GP-C
Product Synonym Names
Recombinant Pre-glycoprotein polyprotein GP complex (GPC); Pre-glycoprotein polyprotein GP complex; Pre-GP-C Cleaved into the following 3 chains: 1. Stable signal peptide; 2. SSP 3. Glycoprotein G1; 4. GP1 5. Glycoprotein G2; 6. GP2
Sequence
LIYKGTYELQ TLELNMETLN MTMPLSCTKN NSHHYIRVGN ETGLELTLTN TSILNHKFCN LSDAHKRNLY DHSLMSIIST FHLSIPNFNQ YEAMSCDFNG GKITVQYNLS HSFAVDAAGH CGTLANGVLQ TFMRMAWGGS YIALDSGRGN WDCIMTSYQY LIIQNTTWDD HCQFSRPSPI GYLGLLSQRT RDIYISRRLL
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C. For extended storage, store at -20 or -80 degree C.
Molecular Weight
26.6
Sequence Positions
59-258aa; Partial
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for P17332
Species
Lassa virus (strain GA391) (LASV)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of GPC recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GPC. It may not necessarily be applicable to this product.

NCBI GI #
138354
NCBI Accession #
P17332.1
UniProt Primary Accession #
P17332
NCBI Official Full Name
Pre-glycoprotein polyprotein GP complex
UniProt Protein Name
Pre-glycoprotein polyprotein GP complex
Protein Family
Pre-glycoprotein polyprotein
UniProt Gene Name
GPC
UniProt Synonym Gene Names
GP-C; Pre-GP-C; SSP; GP1; GP2
UniProt Entry Name
GLYC_LASSG

UniProt Comments for GPC

Function: The stable signal peptide (SSP) is cleaved and functions as a signal peptide. In addition, it is apparently retained as the third component of the GP complex. The SSP is required for efficient glycoprotein expression, post-translational maturation cleavage of GP1 and GP2, glycoprotein transport to the cell surface plasma membrane, formation of infectious virus particles, and acid pH-dependent glycoprotein-mediated cell fusion

By similarity.Glycoprotein G1 mediates virus attachment to host receptor alpha-dystroglycan DAG1. This attachment induces virion internalization predominantly through clathrin- and caveolin-independent endocytosis

By similarity.Glycoprotein G2 is a viral fusion protein. Membrane fusion is mediated by conformational changes induced upon acidification in the endosome

Potential.

Subunit structure: Glycoprotein G1 is a homotetramer; disulfide-linked

Potential. Glycoprotein G2 is a homotetramer

Potential. SSP is a homodimer; disulfide-linked. GP2 homotetramers bind through ionic interactions with GP1 homotetramers to form, with the stable signal peptide, the GP complex. The GP-C polyprotein interacts with host protease MBTPS1/SKI-1; this results in polyprotein processing

By similarity.

Subcellular location: Stable signal peptide: Virion membrane; Multi-pass membrane protein

Potential. Host endoplasmic reticulum membrane; Multi-pass membrane protein

Potential. Host Golgi apparatus membrane; Multi-pass membrane protein

Potential. Host cell membrane; Multi-pass membrane protein

Potential. Glycoprotein G1: Virion membrane; Peripheral membrane protein

Potential. Host endoplasmic reticulum membrane; Peripheral membrane protein

Potential. Host Golgi apparatus membrane; Peripheral membrane protein

Potential. Host cell membrane; Peripheral membrane protein

Potential. Glycoprotein G2: Virion membrane; Single-pass membrane protein

Potential. Host endoplasmic reticulum membrane; Single-pass membrane protein

Potential. Host Golgi apparatus membrane; Single-pass membrane protein

Potential. Host cell membrane; Single-pass membrane protein

Potential. Note: Binding to the stable signal peptide masks endogenous ER localization signals in the cytoplasmic domain of G2 to ensure that only the fully assembled, tripartite GP complex is transported for virion assembly

By similarity.

Domain: The cytoplasmic domain of GP2 plays a role in ER location and binds to the SSP.

Post-translational modification: The SSP remains stably associated with the GP complex following cleavage by signal peptidase and plays crucial roles in the trafficking of GP through the secretory pathway

By similarity.Specific enzymatic cleavages in vivo yield mature proteins. GP-C polyprotein is cleaved in the endoplasmic reticulum by the host protease MBTPS1. Only cleaved glycoprotein is incorporated into virions.

Sequence similarities: Belongs to the arenaviridae GPC protein family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GPC recombinant protein

 Reference Product  PubMed Publications
 FLNA recombinant protein  >12 publications with GPC and FLNA

Pathways associated with GPC recombinant protein

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