Rabbit Anti-EIF4EBP1 Antibody – Cat# MBS125564

Western blot analysis of extracts of 293T and HeLa cells, using Phospho-EIF4EBP1-S65 antibody at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated by 10% FBS after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 10min.

EIF4EBP1 Antibody – Rabbit

Name: EIF4EBP1 Antibody; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS125564
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS125564

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent… More details

$245 / 0.02 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

EIF4EBP1-S65, Antibody

Full Product Name

Phospho-EIF4EBP1-S65 Polyclonal Antibody

Product Gene Name

anti-EIF4EBP1 antibody

Product Synonym Names

Phospho-EIF4EBP1-S65: 4E-BP1; 4EBP1; BP-1; MGC4316; PHAS-I

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Purity/Purification

Affinity Purification

Concentration

1mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

118

OMIM

602223

3D Structure

ModBase 3D Structure for Q13541

Species

Human

Route

Synthetic Peptide

Immunogen

A phospho specific peptide corresponding to residues surrounding S65 of human EIF4EBP1

Product Categories/Family for anti-EIF4EBP1 antibody

Phosphorylation

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-EIF4EBP1 antibody

Western Blot (WB)

Application Notes for anti-EIF4EBP1 antibody

WB: 1:500 - 1:2000

Other Notes

Small volumes of anti-EIF4EBP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EIF4EBP1 purchase

MBS125564 is an antibody targeting EIF4EBP1. Also known as Phospho-EIF4EBP1-S65 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF4EBP1. It may not necessarily be applicable to this product.

NCBI GI #

34921508

NCBI GeneID

1978

NCBI Accession #

Q13541.3

UniProt Primary Accession #

Q13541

UniProt Secondary Accession #

Q6IBN3; B2R502; D3DSW8

UniProt Related Accession #

Q13541

NCBI Official Full Name

Eukaryotic translation initiation factor 4E-binding protein 1

NCBI Official Synonym Full Names

eukaryotic translation initiation factor 4E binding protein 1

NCBI Official Symbol

EIF4EBP1

NCBI Official Synonym Symbols

BP-1; 4EBP1; 4E-BP1; PHAS-I

UniProt Protein Name

Eukaryotic translation initiation factor 4E-binding protein 1

UniProt Synonym Protein Names

Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I

Protein Family

Eukaryotic translation initiation factor 4E-binding protein

UniProt Gene Name

EIF4EBP1

UniProt Synonym Gene Names

4E-BP1; eIF4E-binding protein 1; PHAS-I

UniProt Entry Name

4EBP1_HUMAN

NCBI Protein Information

eukaryotic translation initiation factor 4E-binding protein 1; eIF4E-binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1

NCBI Summary for EIF4EBP1

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]

UniProt Comments for EIF4EBP1

4E-BP1: binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol

Molecular Function: protein binding; translation repressor activity; eukaryotic initiation factor 4E binding

Biological Process: negative regulation of translational initiation; cellular protein metabolic process; TOR signaling pathway; response to ethanol; translation; insulin receptor signaling pathway; translational initiation; gene expression; positive regulation of mitotic cell cycle; negative regulation of protein complex assembly; lung development; G1/S transition of mitotic cell cycle

Research Articles on EIF4EBP1

1. Overexpression of phosphorylated 4E-binding protein 1 is associated with lymph node metastasis in hilar cholangiocarcinoma

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EIF4EBP1 antibody

Reference Product	PubMed Publications
MTOR antibody	>530 publications with EIF4EBP1 and MTOR
EIF4E antibody	>516 publications with EIF4EBP1 and EIF4E
AKT1 antibody	>58 publications with EIF4EBP1 and AKT1
RPTOR antibody	>29 publications with EIF4EBP1 and RPTOR
RHEB antibody	>24 publications with EIF4EBP1 and RHEB
ATM antibody	>12 publications with EIF4EBP1 and ATM
EIF4G1 antibody	>10 publications with EIF4EBP1 and EIF4G1
MLST8 antibody	>5 publications with EIF4EBP1 and MLST8
RPS6KB1 antibody	>5 publications with EIF4EBP1 and RPS6KB1

Pathways associated with anti-EIF4EBP1 antibody

Products by Pathway	Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway antibodies	Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway Diagram
Acute Myeloid Leukemia Pathway antibodies	Acute Myeloid Leukemia Pathway Diagram
Acute Myeloid Leukemia Pathway antibodies	Acute Myeloid Leukemia Pathway Diagram
Alpha6-Beta4 Integrin Signaling Pathway antibodies	Alpha6-Beta4 Integrin Signaling Pathway Diagram
BDNF Signaling Pathway antibodies	BDNF Signaling Pathway Diagram
Cap-dependent Translation Initiation Pathway antibodies	Cap-dependent Translation Initiation Pathway Diagram
Choline Metabolism In Cancer Pathway antibodies	Choline Metabolism In Cancer Pathway Diagram

Diseases associated with anti-EIF4EBP1 antibody

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>48 publications with EIF4EBP1 and Breast Neoplasms
Hypertrophy Antibodies	>43 publications with EIF4EBP1 and Hypertrophy
Cardiovascular Diseases Antibodies	>39 publications with EIF4EBP1 and Cardiovascular Diseases
Disease Models, Animal Antibodies	>37 publications with EIF4EBP1 and Disease Models, Animal
Neoplasm Metastasis Antibodies	>26 publications with EIF4EBP1 and Neoplasm Metastasis
Cell Transformation, Neoplastic Antibodies	>24 publications with EIF4EBP1 and Cell Transformation, Neoplastic
Necrosis Antibodies	>22 publications with EIF4EBP1 and Necrosis
Lung Neoplasms Antibodies	>22 publications with EIF4EBP1 and Lung Neoplasms
Neoplasms, Experimental Antibodies	>22 publications with EIF4EBP1 and Neoplasms, Experimental
Kidney Diseases Antibodies	>21 publications with EIF4EBP1 and Kidney Diseases

Organs/Tissues associated with anti-EIF4EBP1 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>175 publications with EIF4EBP1 and Muscle
Blood Antibodies	>128 publications with EIF4EBP1 and Blood
Vascular Antibodies	>56 publications with EIF4EBP1 and Vascular
Liver Antibodies	>53 publications with EIF4EBP1 and Liver
Brain Antibodies	>47 publications with EIF4EBP1 and Brain
Heart Antibodies	>42 publications with EIF4EBP1 and Heart
Kidney Antibodies	>39 publications with EIF4EBP1 and Kidney
Lung Antibodies	>37 publications with EIF4EBP1 and Lung
Pancreas Antibodies	>24 publications with EIF4EBP1 and Pancreas
Skin Antibodies	>18 publications with EIF4EBP1 and Skin