Rabbit Anti-BCAR1 Polyclonal Antibody – Cat# MBS125673

Western blot analysis of extracts of A-431 cells, using BCAR1 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

BCAR1 Antibody – Rabbit Polyclonal

Name: BCAR1 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS125673
Price: 360 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS125673

BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009] More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

BCAR1, Polyclonal Antibody

Full Product Name

BCAR1 Polyclonal Antibody

Product Gene Name

anti-BCAR1 antibody

Product Synonym Names

p130 Cas: CAS; CAS1; CASS1; CRKAS; P130Cas

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Purity/Purification

Affinity Purification

Concentration

1mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

870

OMIM

602941

3D Structure

ModBase 3D Structure for P56945

Species

Human

Immunogen

Recombinant protein of human BCAR1

Calculated Molecular Weight

93kDa

Product Categories/Family for anti-BCAR1 antibody

Polyclonal

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-BCAR1 antibody

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Application Notes for anti-BCAR1 antibody

WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
IF: 1:20 - 1:50

Other Notes

Small volumes of anti-BCAR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BCAR1 purchase

MBS125673 is an antibody targeting BCAR1. Also known as BCAR1 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BCAR1. It may not necessarily be applicable to this product.

NCBI GI #

288558806

NCBI GeneID

9564

NCBI Accession #

P56945.2

UniProt Primary Accession #

P56945

UniProt Secondary Accession #

B3KWD7; B4DEV4; B4DGB5; B4DIW5; B7Z7X7; E9PCL5; E9PCV2; F5GXA2; F5GXV6; F5H7Z0; F8WA69

UniProt Related Accession #

P56945

NCBI Official Full Name

Breast cancer anti-estrogen resistance protein 1

NCBI Official Synonym Full Names

breast cancer anti-estrogen resistance 1

NCBI Official Symbol

BCAR1

NCBI Official Synonym Symbols

CAS; CAS1; CASS1; CRKAS; P130Cas

UniProt Protein Name

Breast cancer anti-estrogen resistance protein 1

UniProt Synonym Protein Names

CRK-associated substrate; Cas scaffolding protein family member 1; p130cas

Protein Family

Breast cancer anti-estrogen resistance protein

UniProt Gene Name

BCAR1

UniProt Synonym Gene Names

CAS; CASS1; CRKAS

UniProt Entry Name

BCAR1_HUMAN

NCBI Protein Information

breast cancer anti-estrogen resistance protein 1; Crk-associated substrate p130Cas; Cas scaffolding protein family member 1

UniProt Comments for BCAR1

P130Cas: a docking protein containing multiple protein-protein interaction domains. Plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. The amino-terminal SH3 domain regulates its interaction with focal adhesion kinase (FAK) and the FAK-related kinase PYK2 and also with tyrosine phosphatases PTP-1B and PTP-PEST. Overexpression confers antiestrogen resistance on breast cancer cells.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: ruffle; focal adhesion; lamellipodium; cytoplasm; nucleolus; plasma membrane; cytosol; actin cytoskeleton

Molecular Function: signal transducer activity; protein binding; SH3 domain binding; protein kinase binding

Biological Process: epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; antigen receptor-mediated signaling pathway; platelet activation; cell migration; nerve growth factor receptor signaling pathway; actin filament organization; hepatocyte growth factor receptor signaling pathway; platelet-derived growth factor receptor signaling pathway; T cell receptor signaling pathway; regulation of apoptosis; G-protein coupled receptor protein signaling pathway; cell proliferation; B cell receptor signaling pathway; cell division; insulin receptor signaling pathway; regulation of cell growth; cell adhesion; vascular endothelial growth factor receptor signaling pathway; blood coagulation; positive regulation of cell migration

Research Articles on BCAR1

1. Our results show that endogenous Cul5 suppresses epithelial cell transformation by several pathways, including inhibition of Src-Cas-induced ruffling through SOCS6

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BCAR1 antibody

Reference Product	PubMed Publications
CRK antibody	>392 publications with BCAR1 and CRK
SRC antibody	>166 publications with BCAR1 and SRC
PTK2 antibody	>105 publications with BCAR1 and PTK2
PXN antibody	>69 publications with BCAR1 and PXN
FYN antibody	>13 publications with BCAR1 and FYN
PTK2B antibody	>12 publications with BCAR1 and PTK2B
CRKL antibody	>9 publications with BCAR1 and CRKL
PTPN12 antibody	>9 publications with BCAR1 and PTPN12
PTPN1 antibody	>4 publications with BCAR1 and PTPN1

Pathways associated with anti-BCAR1 antibody

Products by Pathway	Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
CDC42 Signaling Events Pathway antibodies	CDC42 Signaling Events Pathway Diagram
CXCR4-mediated Signaling Events Pathway antibodies	CXCR4-mediated Signaling Events Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Downstream Signal Transduction Pathway antibodies	Downstream Signal Transduction Pathway Diagram
EGFR1 Signaling Pathway antibodies	EGFR1 Signaling Pathway Diagram
EPHA2 Forward Signaling Pathway antibodies	EPHA2 Forward Signaling Pathway Diagram

Diseases associated with anti-BCAR1 antibody

Disease Name	Pubmed Publications
Cell Transformation, Neoplastic Antibodies	>34 publications with BCAR1 and Cell Transformation, Neoplastic
Neoplasm Invasiveness Antibodies	>27 publications with BCAR1 and Neoplasm Invasiveness
Neoplasm Metastasis Antibodies	>18 publications with BCAR1 and Neoplasm Metastasis
Disease Models, Animal Antibodies	>10 publications with BCAR1 and Disease Models, Animal
Inflammation Antibodies	>6 publications with BCAR1 and Inflammation
Cardiovascular Diseases Antibodies	>6 publications with BCAR1 and Cardiovascular Diseases
Hypertrophy Antibodies	>4 publications with BCAR1 and Hypertrophy
Hypertension Antibodies	>4 publications with BCAR1 and Hypertension
Kidney Diseases Antibodies	>3 publications with BCAR1 and Kidney Diseases
Liver Neoplasms Antibodies	>3 publications with BCAR1 and Liver Neoplasms

Organs/Tissues associated with anti-BCAR1 antibody

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>40 publications with BCAR1 and Vascular
Muscle Antibodies	>23 publications with BCAR1 and Muscle
Lung Antibodies	>22 publications with BCAR1 and Lung
Bone Antibodies	>18 publications with BCAR1 and Bone
Heart Antibodies	>13 publications with BCAR1 and Heart
Kidney Antibodies	>12 publications with BCAR1 and Kidney
Brain Antibodies	>12 publications with BCAR1 and Brain
Prostate Antibodies	>11 publications with BCAR1 and Prostate
Pancreas Antibodies	>8 publications with BCAR1 and Pancreas
Liver Antibodies	>6 publications with BCAR1 and Liver