NME1 Antibody – Rabbit Polyclonal

The NDK/NME/NM23 kinase family (encoded by the NME gene family) consists of at least eight distinct proteins that exhibit different cellular localization (1). Members of this group inhibit metastasis in a variety of tumor cell types (2). All NDK/NME/NM23 proteins possess nucleoside diphosphatase kinase (NDK) activity and catalyze the phosphorylation of nucleoside diphosphate to the corresponding nucleoside triphosphate to regulate a diverse array of cellular events (3). At least four classes of NDK biochemical activities have been described, including proteinprotein interactions (46), regulation of GTPbinding protein function (79), DNAassociated activities (10,11), and histidinedependent protein phosphotransferase activity (12). NDK/NME proteins participate in the regulation of a broad spectrum of cellular responses, including development, differentiation, proliferation, endocytosis, and apoptosis (13). Because of its role in metastasis suppression and oncogenesis, NDKA (NME1/NM23H1) has been widely studied (14). NDKA (NM23H1) and NDKB (NM23H2) are encoded by adjacent NME1 and NME2 genes and share 90% sequence identity. Two serine residues (Ser122 and Ser144) on NDKA/NM23H1 can be phosphorylated by AMPKalpha1, but only phosphorylation at Ser122 determines whether NDKA channels ATP to AMPKalpha1. This regulates AMPKalpha1 activity towards ACC1, an important regulator of fatty acid metabolism (15). Mutation of NDKB/NM23H2 at Ser122 (S122P) in melanoma cells results in altered phosphoryl transfer activity (16).

1. Lacombe, M.L. et al. (2000) J Bioenerg Biomembr 32, 247-58. 2. Tee, Y.T. et al. (2006) Taiwan J Obstet Gynecol 45, 107-13. 3. Ishikawa, N. et al. (2003) J Bioenerg Biomembr 35, 7-18. 4. Paravicini, G. et al. (1996) Biochem Biophys Res Commun 227, 82-7. 5. Reymond, A. et al. (1999) Oncogene 18, 7244-52. 6. Subramanian, C. et al. (2001) Nat Med 7, 350-5. 7. Zhu, J. et al. (1999) Proc Natl Acad Sci USA 96, 14911-8. 8. Otsuki, Y. et al. (2001) Proc Natl Acad Sci USA 98, 4385-90. 9. Palacios, F. et al. (2002) Nat Cell Biol 4, 929-36. 10. Fan, Z. et al. (2003) Cell 112, 659-72. 11. Postel, E.H. (2003) J Bioenerg Biomembr 35, 31-40. 12. Wagner, P.D. and Vu, N.D. (2000) Biochem J 346 Pt 3, 623-30. 13. Kimura, N. et al. (2000) J Bioenerg Biomembr 32, 309-15. 14. Steeg, P.S. (2004) J Natl Cancer Inst 96, E4. 15. Crawford, R.M. et al. (2006) Mol Cell Biol 26, 5921-31. 16. Schaertl, S. et al. (1999) J Biol Chem 274, 20159-64.

Small volumes of anti-NME1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS125747 is an antibody targeting NME1. Also known as NME1 Polyclonal Antibody. Reactive in Human, mouse, rat. Host: Rabbit.