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Vacuolar-Sorting Protein Dot2 (dot2) Schizosaccharomyces Pombe Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1260988

MBS1260988 is a recombinant protein targeting dot2. Also known as Recombinant Schizosaccharomyces pombe Vacuolar-sorting protein dot2 (dot2). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$920 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Vacuolar-sorting protein dot2 (dot2), Recombinant Protein
Full Product Name
Recombinant Schizosaccharomyces pombe Vacuolar-sorting protein dot2 (dot2)
Product Gene Name
Sequence
MRKRIGIGAL NDDEYLKQYE EVGNELIEQQ SDEIASQLST FQEALKTFAR EHATEIKQNS QFRNTFVKLA LKIGLDPFVS GSDESAWAAV GMNEFYYQVA VRVIEVCYAT QMENGGLLSV SQVCRFLNEE NEAFGHEWLR ETDVVRAVDS LAPLGPGFVL EKIAGKQYIR SLPLELNTDQ NVVLEAVEIL GYVTISILRD NYAWERSRCI QVLNDLVSKS LLWIDSQGVE MAYWGASNLI DDQAQRFLYE NF
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
28,653 Da
Sequence Positions
1-252, Full length
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for O94663
Species
Schizosaccharomyces pombe (strain 972 / 24843) (Fission yeast)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of dot2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for dot2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
GenBank Nucleotide #
NCBI Official Full Name
ESCRT II complex subunit Dot2
NCBI Official Symbol
dot2
UniProt Protein Name
Vacuolar-sorting protein dot2
UniProt Synonym Protein Names
Defective organization of telomere protein 2; ELL-associated protein of 30 kDa homolog dot2
Protein Family
UniProt Gene Name
dot2
NCBI Protein Information
ESCRT II complex subunit Dot2

UniProt Comments for dot2

Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Negatively regulates meiotic spindle pole body maturation via indirect regulation of the pcp1 gene. Required for efficient entry into pre-meiotic S phase.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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