BrdU Antibody – Mouse Monoclonal

The halogenated pyrimidine thymidine analog bromodeoxyuridine (BrdU) is incorporated into newly synthesized DNA strands of S phase cells and is useful for estimating the fraction of cells in S phase. Additionally, the analysis of the uptake of BrdU is a reliable method to quantitate the degree of DNA synthesis. BrdU is also useful for studying sister chromatid exchange and to isolate nascent DNA. UV-induced excision-repair synthesis is one method for incorporating BrdU into cellular DNA. Anti-BrdU antibodies bind to the exposed BrdU in single-stranded DNA after a hydrochloric acid denaturation step or nuclease digestion. Protease antigen recovery is necessary for most tissues or cells fixed with cross-linking agents such as formalin but may decrease the specificity of BrdU immunodetection. The monoclonal antibody Bu20a against BrdU stains BrdU incorporated into the nulcei of a wide range of proliferating cell types including human tumors growing in nude mice and tonsil lymphoid.

Small volumes of anti-BrdU antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS126158 is an antibody targeting BrdU. Also known as BrdU Monoclonal Antibody. Host: Mouse.