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H4K20me3 Antibody – Rabbit

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Popular Catalog #: MBS126181
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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four… More details

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Product Overview

Online Datasheet in PDF
Product Name
H4K20me3, Antibody
Full Product Name
Histone H4K20me3 Polyclonal Antibody
Product Gene Name
anti-H4K20me3 antibody
Product Synonym Names
TriMethyl-Histone H4-K20: H4; H4/n; H4F2; H4FN; FO108; HIST2H4
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Molecular Weight
Calculated MW: 11kDa
Observed MW: 11kDa
OMIM
gene 615069
3D Structure
ModBase 3D Structure for P62805
Immunogen
A synthetic methylated peptide corresponding to residues surrounding K20 of human histone H4

Product Categories/Family for anti-H4K20me3 antibody

Methylation
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-H4K20me3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), CHIP, CHIPseq
Application Notes for anti-H4K20me3 antibody
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
IP: 1:50 - 1:200
CHIP: 1:50 - 1:100
IHC: 1:50-1:200
CHiPseq: 1:20-1:100

Western Blot (WB) of anti-H4K20me3 antibody

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H4-K20 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Rabbit Anti-H4K20me3 Antibody – Cat# MBS126181

Dot Blot (DB) of anti-H4K20me3 antibody

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H4-K20 antibody antibody.
Rabbit Anti-H4K20me3 Antibody, Dot Blot (DB) – Cat# MBS126181

Immunohistochemistry (IHC) of anti-H4K20me3 antibody

Immunohistochemistry of paraffin-embedded rat testis using TriMethyl-Histone H4-K20 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-H4K20me3 Antibody, Immunohistochemistry (IHC) – Cat# MBS126181

Immunohistochemistry (IHC) of anti-H4K20me3 antibody

Immunohistochemistry of paraffin-embedded human kidney cancer using TriMethyl-Histone H4-K20 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-H4K20me3 Antibody, Immunohistochemistry (IHC) – Cat# MBS126181

Immunohistochemistry (IHC) of anti-H4K20me3 antibody

Immunohistochemistry of paraffin-embedded mouse heart using TriMethyl-Histone H4-K20 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-H4K20me3 Antibody, Immunohistochemistry (IHC) – Cat# MBS126181

Immunofluorescence (IF) of anti-H4K20me3 antibody

Immunofluorescence analysis of 293T cells using TriMethyl-Histone H4-K20 antibody. Blue: DAPI for nuclear staining.
Rabbit Anti-H4K20me3 Antibody, Immunofluorescence (IF) – Cat# MBS126181

Histogram of anti-H4K20me3 antibody

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H4-K20 antibody (MBS126181) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Rabbit Anti-H4K20me3 Antibody, Histogram – Cat# MBS126181

Other Notes

Small volumes of anti-H4K20me3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for H4K20me3 purchase

MBS126181 is an antibody targeting H4K20me3. Also known as Histone H4K20me3 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for H4K20me3. It may not necessarily be applicable to this product.

NCBI GI #
51317339
NCBI GeneID
8370
NCBI Accession #
P62805.2
UniProt Primary Accession #
P62805
UniProt Secondary Accession #
P02304; P02305; Q6DRA9; Q6FGB8; Q6NWP7; A2VCL0
UniProt Related Accession #
P62805
NCBI Official Full Name
Histone H4
NCBI Official Synonym Full Names
histone cluster 2, H4a
NCBI Official Symbol
HIST2H4A
NCBI Official Synonym Symbols
H4; H4/n; H4F2; H4FN; FO108; HIST2H4
UniProt Protein Name
Histone H4
UniProt Gene Name
HIST1H4A
UniProt Synonym Gene Names
H4/A; H4FA
UniProt Entry Name
H4_HUMAN
NCBI Protein Information
histone H4; histone 2, H4a; H4 histone, family 2; histone IV, family 2; H4 histone family, member N

UniProt Comments for H4K20me3

H4: histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The gene encoding H4 is intronless.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p22.2

Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus

Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: chromatin silencing at rDNA; establishment and/or maintenance of chromatin architecture; DNA replication-dependent nucleosome assembly; regulation of gene expression, epigenetic; negative regulation of megakaryocyte differentiation; nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; DNA replication-independent nucleosome assembly; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; mitotic cell cycle; DNA methylation on cytosine; telomere maintenance
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Research Articles on H4K20me3

1. NASP balances the activity of the heat shock proteins Hsc70 and Hsp90 to direct H3-H4 for degradation by chaperone-mediated autophagy

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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