S2448 (MTOR) Antibody – Rabbit

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]

mTOR: an atypical kinase belonging to the PIKK family of kinases. Is the catalytic subunit of two protein complexes, mTORC1 and mTORC2. mTORC1 activates S6K and inactivates 4E-BP1, up-regulating protein synthesis. mTORC1 contains Raptor, a positive regulatory subunit and scaffold for recruiting substrates, two negative regulators, PRAS40 and DEPTOR, and mLST8; it is a target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. mTORC2, a downstream effector of PI3K, is insensitive to rapamycin and activates Akt by phosphorylating a key activation site. mTORC2 contains regulatory subunits Rictor and mSIN1, PROTOR, mLST8, and the negative regulator DEPTOR. mTORC1 suppresses PI3K activity via a strong negative feedback loop that involves S6K1. Inhibiting mTORC1 ablates this negative feedback loop and potentiates PI3K signaling. Known inhibitors of mTOR include rapamycin, temsirolimus (CCI-779).

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Autophagy; Motility/polarity/chemotaxis; Protein kinase, atypical; ATYPICAL group; PIKK family; FRAP subfamily

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: PML body; endoplasmic reticulum membrane; lysosomal membrane; lysosome; dendrite; endomembrane system; cytosol; Golgi membrane; nucleoplasm; mitochondrial outer membrane; cell soma; membrane; cytoplasm; TORC2 complex; phosphoinositide 3-kinase complex; nucleus

Molecular Function: protein dimerization activity; protein domain specific binding; protein serine/threonine kinase activity; protein binding; phosphoprotein binding; ribosome binding; drug binding; kinase activity; ATP binding

Biological Process: negative regulation of autophagy; regulation of myelination; TOR signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of translation; protein amino acid autophosphorylation; regulation of glycogen biosynthetic process; negative regulation of cell size; signal transduction; protein amino acid phosphorylation; germ cell development; negative regulation of macroautophagy; cellular response to nutrient levels; positive regulation of stress fiber formation; regulation of carbohydrate utilization; response to stress; protein catabolic process; cell growth; response to nutrient; regulation of response to food; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; peptidyl-threonine phosphorylation; positive regulation of lipid biosynthetic process; response to amino acid stimulus; negative regulation of NFAT protein import into nucleus; double-strand break repair via homologous recombination; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; T cell costimulation; ruffle organization and biogenesis; insulin receptor signaling pathway; innate immune response; regulation of fatty acid beta-oxidation; positive regulation of transcription from RNA polymerase III promoter; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; regulation of protein kinase activity; vascular endothelial growth factor receptor signaling pathway; phosphorylation; growth