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H3R17me2a Antibody – Rabbit

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS126208
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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is… More details

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Product Overview

Online Datasheet in PDF
Product Name
H3R17me2a, Antibody
Full Product Name
Histone H3R17me2a Polyclonal Antibody
Product Gene Name
anti-H3R17me2a antibody
Product Synonym Names
Asymmetric DiMethyl-Histone H3-R17: HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Other (Wide Range)
Purity/Purification
Affinity Purification
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
OMIM
gene 602819
3D Structure
ModBase 3D Structure for P68431
Calculated MW
15kDa
Observed MW
17kDa
Immunogen
A synthetic methylated peptide corresponding to residues surrounding Arg17 of human histone H3
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Product Categories/Family for anti-H3R17me2a antibody

Methylation
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-H3R17me2a antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-H3R17me2a antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200

Western Blot (WB) of anti-H3R17me2a antibody

Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R17 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Rabbit Anti-H3R17me2a Antibody – Cat# MBS126208

Dot Blot (DB) of anti-H3R17me2a antibody

Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R17 antibody.
Rabbit Anti-H3R17me2a Antibody, Dot Blot (DB) – Cat# MBS126208

Immunohistochemistry (IHC) of anti-H3R17me2a antibody

Immunohistochemistry of paraffin-embedded rat lung using Asymmetric DiMethyl-Histone H3-R17 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-H3R17me2a Antibody, Immunohistochemistry (IHC) – Cat# MBS126208

Immunohistochemistry (IHC) of anti-H3R17me2a antibody

Immunohistochemistry of paraffin-embedded human lung cancer using Asymmetric DiMethyl-Histone H3-R17 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-H3R17me2a Antibody, Immunohistochemistry (IHC) – Cat# MBS126208

Immunohistochemistry (IHC) of anti-H3R17me2a antibody

Immunohistochemistry of paraffin-embedded human kidney cancer using Asymmetric DiMethyl-Histone H3-R17 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-H3R17me2a Antibody, Immunohistochemistry (IHC) – Cat# MBS126208

Immunohistochemistry (IHC) of anti-H3R17me2a antibody

Immunohistochemistry of paraffin-embedded mouse lung using Asymmetric DiMethyl-Histone H3-R17 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-H3R17me2a Antibody, Immunohistochemistry (IHC) – Cat# MBS126208

Immunofluorescence (IF) of anti-H3R17me2a antibody

Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R17 antibody. Blue: DAPI for nuclear staining.
Rabbit Anti-H3R17me2a Antibody, Immunofluorescence (IF) – Cat# MBS126208

Immunohistochemistry (IHC) of anti-H3R17me2a antibody

Immunohistochemistry of paraffin-embedded rat testis using H3R177me2a antibody (MBS126208) at dilution of 1:100 (40x lens).
Rabbit Anti-H3R17me2a Antibody, Immunohistochemistry (IHC) – Cat# MBS126208

Immunohistochemistry (IHC) of anti-H3R17me2a antibody

Immunohistochemistry of paraffin-emebedded human placenta using H3R17me2a antibody (MBS126208) at dilution of 1:100 (40x lens).
Rabbit Anti-H3R17me2a Antibody, Immunohistochemistry (IHC) – Cat# MBS126208

Other Notes

Small volumes of anti-H3R17me2a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for H3R17me2a purchase

MBS126208 is an antibody targeting H3R17me2a. Also known as Histone H3R17me2a Polyclonal Antibody. Reactive in Human, Mouse, Rat, Other (Wide Range). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for H3R17me2a. It may not necessarily be applicable to this product.

NCBI GI #
55977055
NCBI GeneID
8356
NCBI Accession #
P68431.2
UniProt Primary Accession #
P68431
UniProt Secondary Accession #
P02295; P02296; P16106; Q6ISV8; Q6NWP8; Q6NWP9; Q6NXU4; Q71DJ3; Q93081; A0PJT7; A5PLR1
UniProt Related Accession #
P68431
NCBI Official Full Name
Histone H3.1
NCBI Official Synonym Full Names
histone cluster 1, H3j
NCBI Official Symbol
HIST1H3J
NCBI Official Synonym Symbols
H3/j; H3FJ
UniProt Protein Name
Histone H3.1
UniProt Synonym Protein Names
Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
UniProt Gene Name
HIST1H3A
UniProt Synonym Gene Names
H3FA
UniProt Entry Name
H31_HUMAN
NCBI Protein Information
histone H3.1; histone H3/j; histone 1, H3j; H3 histone family, member J

NCBI Summary for H3R17me2a

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
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UniProt Comments for H3R17me2a

H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p22.2

Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus

Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; blood coagulation; DNA methylation on cytosine; DNA replication-dependent nucleosome assembly; regulation of gene expression, epigenetic
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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