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H3K27me2 Antibody – Rabbit

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS126234
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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core… More details

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Product Overview

Online Datasheet in PDF
Product Name
H3K27me2, Antibody
Full Product Name
Histone H3K27me2 Polyclonal Antibody
Product Gene Name
anti-H3K27me2 antibody
Product Synonym Names
H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; DiMethyl-Histone H3-K27
Isotype
IgG
Host
Rabbit
Form/Format
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Purity/Purification
Affinity Purification
Preparation and Storage
Store at -20°C. Avoid repeated freeze/thaw cycles
Molecular Weight
Observed MW: 17kDa
Calculated MW: 16kDa
OMIM
602820
3D Structure
ModBase 3D Structure for Q16695
Cross-Reactivity
Human, Mouse, Rat (Wide Range Predicted)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human histone H3 (NP_003520.1).

Product Categories/Family for anti-H3K27me2 antibody

Methylation
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-H3K27me2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Dot Blot (DB), ELISA (E), ChIP, ChIPseq
Application Notes for anti-H3K27me2 antibody
WB 1:500 - 1:1000
DB 1:500 - 1:1000
IHC-P 1:50 - 1:200
IF/ICC 1:50 - 1:200
ELISA: Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
ChIP: 5µg antibody for 5µg-10µg of Chromatin
ChIP-seq 1:20 - 1:100

Western Blot (WB) of anti-H3K27me2 antibody

Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K27 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Rabbit Anti-H3K27me2 Antibody – Cat# MBS126234

Dot Blot (DB) of anti-H3K27me2 antibody

Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K27 antibody at 1:1000 dilution.
Rabbit Anti-H3K27me2 Antibody, Dot Blot (DB) – Cat# MBS126234

Immunohistochemistry (IHC) of anti-H3K27me2 antibody

Immunohistochemistry of paraffin-embedded rat lung using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-H3K27me2 Antibody, Immunohistochemistry (IHC) – Cat# MBS126234

Immunohistochemistry (IHC) of anti-H3K27me2 antibody

Immunohistochemistry of paraffin-embedded human kidney using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-H3K27me2 Antibody, Immunohistochemistry (IHC) – Cat# MBS126234

Immunohistochemistry (IHC) of anti-H3K27me2 antibody

Immunohistochemistry of paraffin-embedded human lung cancer using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-H3K27me2 Antibody, Immunohistochemistry (IHC) – Cat# MBS126234

Immunohistochemistry (IHC) of anti-H3K27me2 antibody

Immunohistochemistry of paraffin-embedded human liver cancer using DiMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens).
Rabbit Anti-H3K27me2 Antibody, Immunohistochemistry (IHC) – Cat# MBS126234

Immunohistochemistry (IHC) of anti-H3K27me2 antibody

Immunohistochemistry of paraffin-embedded Mouse heart using DiMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-H3K27me2 Antibody, Immunohistochemistry (IHC) – Cat# MBS126234

Immunohistochemistry (IHC) of anti-H3K27me2 antibody

Immunohistochemistry of paraffin-embedded Mouse kidney using DiMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-H3K27me2 Antibody, Immunohistochemistry (IHC) – Cat# MBS126234

Immunohistochemistry (IHC) of anti-H3K27me2 antibody

Immunohistochemistry of paraffin-embedded Mouse lung using DiMethyl-Histone H3-K27 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-H3K27me2 Antibody, Immunohistochemistry (IHC) – Cat# MBS126234

Immunohistochemistry (IHC) of anti-H3K27me2 antibody

Immunohistochemistry of paraffin-embedded Rat brain using DiMethyl-Histone H3-K27 antibody(MBS126234) atdilution of 1:100 (40x lens).
Rabbit Anti-H3K27me2 Antibody, Immunohistochemistry (IHC) – Cat# MBS126234

Immunohistochemistry (IHC) of anti-H3K27me2 antibody

Immunohistochemistry of paraffin-embedded Rat kidney using DiMethyl-Histone H3-K27 antibody (MBS126234) at dilution of 1:100 (40x lens).
Rabbit Anti-H3K27me2 Antibody, Immunohistochemistry (IHC) – Cat# MBS126234

Immunohistochemistry (IHC) of anti-H3K27me2 antibody

Immunohistochemistry of paraffin-embedded Rat liver using DiMethyl-Histone H3-K27 antibody (MBS126234) atdilution of 1:100 (40x lens).
Rabbit Anti-H3K27me2 Antibody, Immunohistochemistry (IHC) – Cat# MBS126234

Immunoflouorescence (IF) of anti-H3K27me2 antibody

Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K27 antibody (MBS126234).Blue: DAPI for nuclear staining.
Rabbit Anti-H3K27me2 Antibody, Immunoflouorescence (IF) – Cat# MBS126234

Testing Data (TD) of anti-H3K27me2 antibody

Chromatin immunoprecipitation analysis extracts of 293 cell line, using DiMethylHistone H3-K27 antibody (MBS126234) and rabbit igG . The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Rabbit Anti-H3K27me2 Antibody, Testing Data (TD) – Cat# MBS126234

Western Blot (WB) of anti-H3K27me2 antibody

Western blot analysis of various lysates, using DiMethyl-Histone H3-K27 Rabbit pAb (MBS126234) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.
Rabbit Anti-H3K27me2 Antibody, Western Blot (WB) – Cat# MBS126234

Dot Blot (DB) of anti-H3K27me2 antibody

Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K27 antibody (MBS126234) at 1:1000 dilution.
Rabbit Anti-H3K27me2 Antibody, Dot Blot (DB) – Cat# MBS126234

Immunohistochemistry (IHC) of anti-H3K27me2 antibody

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using DiMethyl-Histone H3-K27 Rabbit pAb (MBS126234) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Rabbit Anti-H3K27me2 Antibody, Immunohistochemistry (IHC) – Cat# MBS126234

Immunohistochemistry (IHC) of anti-H3K27me2 antibody

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using DiMethyl-Histone H3-K27 Rabbit pAb (MBS126234) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining
Rabbit Anti-H3K27me2 Antibody, Immunohistochemistry (IHC) – Cat# MBS126234

Immunohistochemistry (IHC) of anti-H3K27me2 antibody

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using DiMethyl-Histone H3-K27 Rabbit pAb (MBS126234) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Rabbit Anti-H3K27me2 Antibody, Immunohistochemistry (IHC) – Cat# MBS126234

Immunoflouorescence (IF) of anti-H3K27me2 antibody

Immunofluorescence analysis of HeLa cells using DiMethyl-Histone H3-K27 Rabbit pAb (MBS126234) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (MBS128190) at 1:500 dilution. Blue: DAPI for nuclear staining.
Rabbit Anti-H3K27me2 Antibody, Immunoflouorescence (IF) – Cat# MBS126234

Other Notes

Small volumes of anti-H3K27me2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for H3K27me2 purchase

MBS126234 is an antibody targeting H3K27me2. Also known as Histone H3K27me2 Polyclonal Antibody. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for H3K27me2. It may not necessarily be applicable to this product.

NCBI GI #
18202512
NCBI GeneID
8290
NCBI Accession #
Q16695.3
UniProt Primary Accession #
Q16695
UniProt Secondary Accession #
Q6FGU4; B2R5K3
UniProt Related Accession #
Q16695
NCBI Official Full Name
Histone H3.1t
NCBI Official Synonym Full Names
histone cluster 3, H3
NCBI Official Symbol
HIST3H3
NCBI Official Synonym Symbols
H3t; H3.4; H3/g; H3FT
UniProt Protein Name
Histone H3.1t
UniProt Synonym Protein Names
H3/g
UniProt Gene Name
HIST3H3
UniProt Synonym Gene Names
H3FT; H3/t; H3t
UniProt Entry Name
H31T_HUMAN
NCBI Protein Information
histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T

NCBI Summary for H3K27me2

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
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UniProt Comments for H3K27me2

HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance
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Research Articles on H3K27me2

1. Deregulation of histone H3 trimethylation at lysine 27 in diffuse large B-cell lymphoma may be another high-risk phenotype

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-H3K27me2 antibody

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 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 Condensation Of Prophase Chromosomes Pathway antibodies  Condensation Of Prophase Chromosomes Pathway Diagram
 DNA Damage/Telomere Stress Induced Senescence Pathway antibodies  DNA Damage/Telomere Stress Induced Senescence Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram

Diseases associated with anti-H3K27me2 antibody

 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >1 publications with H3K27me2 and Fibrosis
 Cell Transformation, Neoplastic Antibodies  >1 publications with H3K27me2 and Cell Transformation, Neoplastic
 Necrosis Antibodies  >1 publications with H3K27me2 and Necrosis
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